The Journal of Contemporary Dental Practice

Register      Login

SEARCH WITHIN CONTENT

FIND ARTICLE

Volume / Issue

Online First

Archive
Related articles

VOLUME 13 , ISSUE 3 ( May-June, 2012 ) > List of Articles

ORIGINAL RESEARCH

Antimicrobial Effect of Conventional Root Canal Medicaments vs Propolis against Enterococcus faecalis, Staphylococcus aureus and Candida albicans

Sudha Mattigatti, Deepak Jain, P Ratnakar, Shridhar Moturi, Sarath Varma, Surabhi Rairam

Citation Information : Mattigatti S, Jain D, Ratnakar P, Moturi S, Varma S, Rairam S. Antimicrobial Effect of Conventional Root Canal Medicaments vs Propolis against Enterococcus faecalis, Staphylococcus aureus and Candida albicans. J Contemp Dent Pract 2012; 13 (3):305-309.

DOI: 10.5005/jp-journals-10024-1142

Published Online: 01-06-2012

Copyright Statement:  Copyright © 2012; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Aim

To evaluate and compare antimicrobial effect of various root canal medicaments against Enterococcus faecalis, Staphylococcus aureus and Candida albicans.

Materials and methods

Six root canal medicaments: 2% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX) Calcium hydroxide (Ca(OH)2), EDTA, MTAD and propolis and three microorganisms: Staphylococcus aureus, Enterococcus faecalis and Candida albicans were used. These strains were inoculated in brain heart infusion (BHI) and incubated at 37 degrees C for 24 hours. For the agar diffusion test (ADT), petri plates with 20 ml of BHI agar were inoculated with 0.1 ml of the microbial suspensions, using sterile swabs that were spread on the medium, obtaining growth injunction. Paper disks were immersed in the experimental solutions for 1 minute. Subsequently, four papers disks containing one of the substances were placed on the BHI agar surface in each agar plate. The plates were incubated at 37°C for 48 hours. The diameter of microbial inhibition was measured around the papers disks containing the substances. One way ANOVA followed by Tukey's post-hoc test were used. p-value <0.05 was considered statistically significant.

Results

Propolis and other irrigants were found to be effective on C. albicans, S. aureus and E. faecalis. CHX and MTAD were found to be most effective amongst all the materials tested followed by propolis.

Conclusion

Propolis showed antimicrobial activity against E. faecalis, S. aureus, C. albicans. It appears that propolis is an effective intracanal irrigant in eradicating E. faecalis and C. albicans.

Clinical significance

Propolis is an effective intracanal irrigant in eradicating E. faecalis and C. albicans. It could be used as an alternative intracanal medicament.

How to cite this article

Mattigatti S, Jain D, Ratnakar P, Moturi S, Varma S, Rairam S. Antimicrobial Effect of Conventional Root Canal Medicaments vs Propolis Against Enterococcus faecalis, Staphylococcus aureus and Candida albicans. J Contemp Dent Pract 2012;13(3):305-309.


PDF Share
  1. Short-term antibacterial activity of root canal sealers towards Enterococcus faecalis. International Endodontic Journal 2005;38:483-88.
  2. Antibacterial efficacy of calcium hydroxide intracanal dressing: A systematic review and meta-analysis. International Endodontic Journal 2007;40:2-10.
  3. Evaluation of residual antibacterial activity of three concentrations of new root canal irrigation solution. NYSDJ 2008;31-33.
  4. Avaliação da atividade antibacteriana de medicação intracal–três bases fortes e pastas à base de hidróxido de cálcio e paramonoclorofenol canforado. RGO 1996;44(5):271-74.
  5. Isolamento de candida em canais radiculares e verificação da sua sensibilidade a medicamentos utilizados na prática endodôntica. Rev Odontol UNICID 1997;9(2):119-30.
  6. Intraradicular bacteria and fungi in root-filled, assymptomatic human teeth with therapyresistant periapical lesions: A long-term light and electron microscope follow-up study. J Endod 1990;16(12):580-88.
  7. Candida in root canal in accordance with oral ecology. Int Endod J 1992;25(1):40.
  8. Identificação e análise dos microorganismos presentes em canais radiculares com mortificação pulpar. Rev Paul Odontol 2006;29(1):38-41.
  9. Antimicrobial activity of chlorophyll-based solution on Candida albicans and Enterococcus faecalis. Revista Sul-Brasileira de Odontologia, 2007;4(2):36-40.
  10. Chemical compositions and antimicrobial activities of four different Anatolian propolis samples. Microbiol Res 2005;160:189-95.
  11. Histological analysis of rat dental pulp tissue capped with Propolis. J Oral Sci 2005;47:135-38.
  12. A quantitative analysis of propolis: A promising new storage media following avulsion. Dent Traumatol 2004;20:85-89.
  13. The influence of novel propolis on mutans Streptococci biofilms and caries development in rats. Arch Oral Biol 2006;51:15-22.
  14. The effect of propolis on dentinal hypersensitivity and level of satisfaction among patients from a university hospital Riyadh, Saudi Arabia. Indian J Dent Res 1999;10:130-37.
  15. Guideline on pulp therapy for primary and young permanent teeth. Pediatr Dent 2004;26(7 Suppl):115-19.
  16. The antibacterial effect of camphorated paramonochlorophenol, camphorated phenol and calcium hydroxide in the treatment of infected root canals. End Dent Traumatol, Oct 1985;l(5):170-75.
  17. Bacteriologic evaluation of the effect of 0.5 percent sodium hypochlorite in endodontic therapy. Oral Surg Oral Med Oral Pathol, Mar 1983;55(3):307-12.
  18. Evaluation of antimicrobial effect of root canal sealers. Pakistan Oral and Dental J 2011;31(2).
  19. Two methods to evaluate the antimicrobial action of calcium hydroxide paste. J Endod, Dec 2001;27(12):720-23.
  20. Antibacterial properties of propolis (bee glue). J Royal Soci Med 1990;83:159.
  21. Bioautographische erfassung der antimikrobiell wirksamen Inhaltstoffe von propolis. Pharmazie 1975;30:799.
  22. Inhibition of bacterial DNAdependent RNA polymerases and restriction endonucleases by UV-absorbing components from propolis. Pharmazie 1986;41:131-32.
  23. al. Effect of Mexican propolis extracts from Apis mellifera on Candida albicans in vitro growth. Rev Iberoam Micol, Mar 2008;25(1):22-26.
  24. Comparative evaluation of propolis and triantibiotic mixture as an intracanal medicament against Enterococcus faecalis. J Endod, Sep 2011;37(9):1287-89.
  25. Antibacterial activity of propolis versus conventional endodontic disinfectants against Enterococcus faecalis in infected dentinal tubules. J Endod, Mar 2011;37(3):376-81.
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.