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VOLUME 13 , ISSUE 4 ( July-August, 2012 ) > List of Articles


Grape Seed Extract as a Potential Remineralizing Agent: A Comparative in vitro Study

Shiny Benjamin, Roshni, Sabeena Susan Thomas, Mohan Thomas Nainan

Citation Information : Benjamin S, R, Thomas SS, Nainan MT. Grape Seed Extract as a Potential Remineralizing Agent: A Comparative in vitro Study. J Contemp Dent Pract 2012; 13 (4):425-430.

DOI: 10.5005/jp-journals-10024-1162

Published Online: 01-12-2012

Copyright Statement:  Copyright © 2012; The Author(s).



Remineralization is an effective treatment that may stop or reverse early tooth decay. Grape seed extract (GSE) is the potential remineralizing agent under investigation.

Materials and methods

Sound human tooth sections were obtained from the cervical portion of the root and stored in demineralizing solution at 37°C for 96 hours to induce artificial root caries lesions. The sections were divided into four treatment groups including 6.5% grape seed extract, sodium monofluorophosphate (220 ppm) with 0.05% calcium glycerophosphate, 0.5% calcium glycerophosphate and control (no treatment). An in vitro pH cycling model was used to cycle the demineralized specimens through treatment solutions, acidic buffer and neutral buffer for 8 days at 6 cycles per day. Subsequently, they were evaluated using confocal laser scanning microscope. Data were analyzed using analysis of variance (p < 0.05).


GSE revealed less demineralization and more remineralization compared with other groups.


GSE promotes remineralization of artificial root caries lesions.

Clinical significance

The search for the perfect remineralizing agent continues to this day. GSE could be a welcome addition to the remineralization armamentarium.

Abbreviations and acronyms

GSE: Grape seed extract; ppm: Parts per million; CaGP: Calcium glycerophosphate; CLSM: Confocal laser scanning microscope; ANOVA: Analysis of variance; PA: Proanthocyanidin; CEJ: Cementoenamel junction; mM: Millimole; CaCl2.2H2O: Calcium chloride dihydrate; KH2PO4: Potassium dehydrate phosphate; K2HPO4: Dipotassium phosphate; dH2O: Deionized water; w/v: Weight by volume; ROD: Relative optical density; nm: Nanometer; SD: Standard deviation.

How to cite this article

Benjamin S, Roshni, Thomas SS, Nainan MT. Grape Seed Extract as a Potential Remineralizing Agent: A Comparative in vitro Study. J Contemp Dent Pract 2012;13(4):425-430.

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