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VOLUME 13 , ISSUE 4 ( July-August, 2012 ) > List of Articles

RESEARCH ARTICLE

Grape Seed Extract as a Potential Remineralizing Agent: A Comparative in vitro Study

Shiny Benjamin, Roshni, Sabeena Susan Thomas, Mohan Thomas Nainan

Citation Information : Benjamin S, R, Thomas SS, Nainan MT. Grape Seed Extract as a Potential Remineralizing Agent: A Comparative in vitro Study. J Contemp Dent Pract 2012; 13 (4):425-430.

DOI: 10.5005/jp-journals-10024-1162

Published Online: 01-08-2012

Copyright Statement:  Copyright © 2012; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Objective

Remineralization is an effective treatment that may stop or reverse early tooth decay. Grape seed extract (GSE) is the potential remineralizing agent under investigation.

Materials and methods

Sound human tooth sections were obtained from the cervical portion of the root and stored in demineralizing solution at 37°C for 96 hours to induce artificial root caries lesions. The sections were divided into four treatment groups including 6.5% grape seed extract, sodium monofluorophosphate (220 ppm) with 0.05% calcium glycerophosphate, 0.5% calcium glycerophosphate and control (no treatment). An in vitro pH cycling model was used to cycle the demineralized specimens through treatment solutions, acidic buffer and neutral buffer for 8 days at 6 cycles per day. Subsequently, they were evaluated using confocal laser scanning microscope. Data were analyzed using analysis of variance (p < 0.05).

Results

GSE revealed less demineralization and more remineralization compared with other groups.

Conclusion

GSE promotes remineralization of artificial root caries lesions.

Clinical significance

The search for the perfect remineralizing agent continues to this day. GSE could be a welcome addition to the remineralization armamentarium.

Abbreviations and acronyms

GSE: Grape seed extract; ppm: Parts per million; CaGP: Calcium glycerophosphate; CLSM: Confocal laser scanning microscope; ANOVA: Analysis of variance; PA: Proanthocyanidin; CEJ: Cementoenamel junction; mM: Millimole; CaCl2.2H2O: Calcium chloride dihydrate; KH2PO4: Potassium dehydrate phosphate; K2HPO4: Dipotassium phosphate; dH2O: Deionized water; w/v: Weight by volume; ROD: Relative optical density; nm: Nanometer; SD: Standard deviation.

How to cite this article

Benjamin S, Roshni, Thomas SS, Nainan MT. Grape Seed Extract as a Potential Remineralizing Agent: A Comparative in vitro Study. J Contemp Dent Pract 2012;13(4):425-430.


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  1. Minimal intervention: A new concept for operative dentistry. Quintessence Int 2000;31:527-33.
  2. The fine structure of human cementum. Acta Odontol Scand 1965;23:423-41.
  3. The mineral phase of sound and carious human dental cementum studied by electron microscopy. Acta Odontol Scand 1970;28:305-22.
  4. Further observations on the fine structure of orally exposed and carious human cementum. Arch Oral Biol 1971;16:71-85.
  5. In vitro remineralisation of severely compromised bonded dentin. J Dent Res 2010;89:405-10.
  6. In vitro remineralisation effects of grape seed extract on artificial root caries. J Dent 2008;36:900-06.
  7. Plant polyphenols and their anti-cariogenic properties: A review. Molecules 2011;16:1486-507.
  8. Grape products and oral health. Grapes and Health J Nutr 2009;139(9) (Suppl) S1818-23.
  9. Measurement of enamel remineralisation using microradiography and confocal microscopy. A correlational study. Caries Res 1998;32:385-92.
  10. Effect of tea polyphenols on glucan synthesis by glucosyltransferases from Streptococcus mutans. Chem Pharm Bull 1990;38:717-20.
  11. In vitro antimicrobial activity of Caesalpinia ferra Martius fruits against oral pathogens. J Ethnopharmacol 2009;15:289-94.
  12. Chemical characterisation of red wine grape (Vitis vinifera and Vitis interspecific hybrids) and pomace phenolic extracts and their biological activity against Streptococcus mutans. J Agric Food Chem 2007;55,10200-207.
  13. Professional topical fluoride applications – clinical efficiency and mechanism of action. Adv Dent Res 1994;8:190-201.
  14. Remineralisation of deep enamel dentine caries lesions. Aust Dent J 2008;53:281-85.
  15. The cariostatic effect of calcium glycerophophate in monkeys. Caries Res 1972;6:43-51.
  16. Increased plaque calcium and phosphorous concentrations after using a calcium carbonate toothpaste containing calcium glycerophosphate and sodium monofluorophosphate. Caries Res 1979;13:57-59.
  17. Desorption of salivary proteins from hydroxyapatite by phytic acid and glycerophosphate and the plaque inhibiting effect of the two compounds in vivo. J Dent Res 1972;51:800-11.
  18. Use of high performance liquid chromatography techniques to study the protection of hydroxyapatite by fluoride and glycerophosphate against demineralization in vitro. Caries Res 1980;14:221-32.
  19. Microradiography and confocal laser scanning microscopy applied to enamel lesions formed in vivo with and without fluoride varnish treatment. Eur J oral Sci 1996;104:378-83.
  20. The application of in vitro models to research on demineralisation and remineralisation of the teeth. Adv Dent Res 1995;9:175-93.
  21. Single section model for enamel de-and remineralisation studies. I. The effects of different Ca/P ratios in remineralisation solutions. J Dent Res 1993;72:1599-603.
  22. Structural aspects of dental caries. J Dent Res 1974;53:165-78.
  23. PH cycling models for in vitro evaluation of the efficiency of fluoridated dentrifices for caries control: strengths and limitations. J Appl Oral Sci 2010;18:316-34.
  24. Effect of calcium glycerophosphate on demineralisation in an in vitro biofilm model. Caries Res 2006;40:142-47.
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