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VOLUME 14 , ISSUE 2 ( March-April, 2013 ) > List of Articles


An in vitro Evaluation of Microtensile Bond Strength of Resin-based Sealer with Dentin Treated with Diode and Nd:YAG Laser

G Anil Kumar, Maneesha Das, Sindhu Ramesh, Surendranath Garapati

Citation Information : Kumar GA, Das M, Ramesh S, Garapati S. An in vitro Evaluation of Microtensile Bond Strength of Resin-based Sealer with Dentin Treated with Diode and Nd:YAG Laser. J Contemp Dent Pract 2013; 14 (2):183-187.

DOI: 10.5005/jp-journals-10024-1297

Published Online: 01-08-2013

Copyright Statement:  Copyright © 2013; The Author(s).



Smear layer is a negative factor which prevents adhesion of the filling material to the dentinal walls. Recent advances in dental research have incorporated lasers as a potential adjunct in root canal treatment by removing the smear layer before filling the root canal system, enhancing the adhesion of sealers to dentin and improving the sealing ability.


To evaluate the microtensile bond strength of AH-Plus resin-based sealer to dentin after treatment with 980 nm diode and 1,064 nm neodymium-doped:yttrium aluminum garnet (Nd:YAG) laser in vitro.

Materials and methods

Thirty specimens prepared for three groups namely group I (control), group II (980 nm diode–lased specimens) and group III (Nd:YAG–lased specimens). One tooth from each group was observed under scanning electron microscope for evaluation of intracanal root dentin morphology. Remaining specimens were used for making microsections by hard tissue microtome. Specimens for groups II and III were lased with 980 nm diode and 1,064 nm Nd:YAG laser. AH Plus sealer was applied onto specimens and mounted onto Instron universal testing machine for microtensile bond strength testing. Results were subjected to statistical analysis using one-way analysis of variance (ANOVA) and Tukey's test.


Group III Nd:YAG had maximum mean microtensile bond strength values (11.558 ± 0.869), followed by group II diode (9.073 ± 0.468) and group I control (6.05 ± 0.036). Statistically significant differences were seen among all the groups. SEM analysis shows removal of smear layer in both groups II and III.


Both Nd:YAG and diode laser were more effective than control group in improving the microtensile bond strength of AH Plus sealer to dentin.

Clinical significance

Lasers have the potential to increase the adhesiveness of root canal sealer to dentin surface, thereby improving the quality of root canal obturation.

How to cite this article

Maneesha D, Anil KG, Sindhu R, Surendranath G, Deepak S. An in vitro Evaluation of Microtensile Bond Strength of Resin-based Sealer with Dentin Treated with Diode and Nd:YAG Laser. J Contemp Dent Pract 2013;14(2): 183-187.

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  1. Evaluation of apical microleakage of teeth sealed with four different root canal sealers. J Appl Oral Sci 2006;14(5):341-45.
  2. Bond strength of AH Plus and Epiphany sealers on root dentin irradiated with 980 nm diode laser. Int End J 2008;41:733-40.
  3. Adhesion of Epiphany and AH Plus sealers to human root dentin treated with different solutions. Braz Dent J 2008;19(1):46-50.
  4. A histopathological study of the morphological changes at the apical seat and in the periapical region after irradiation with a pulsed Nd:YAG laser. Int End J 1998;31:415-20.
  5. Ex vivo study of the adhesion of an epoxy-based sealer to human dentin submitted to irradiation with Er:YAG and Nd:YAG lasers. Int End J 2005;38:866-70.
  6. Bactericidal effect of different laser systems in the deep layers of dentin. Lasers Surg Med 2004;35:111-16.
  7. Adhesion testing of dentin bonding agents: A review. Dent Mater 1995;11:117-25.
  8. Permeability, morphologic and temperature changes of canal dentin walls induced by Nd:YAG, CO2 and argon lasers. Int End J 1996;29:13-22.
  9. The sealing ability of an epoxy-resin root canal sealer after Nd:YAG laser irradiation of the root canal. Int End J 2005;38:302-09.
  10. Scanning electron microscopic study of dentin lased with Argon, CO2 and Nd:YAG Laser. J Endod 1998;24(2):77-81.
  11. Effects of pulsed Nd:YAG Laser irradiation on root canal wall dentin with different laser initiators. J Endod 1998;24(5):352-55.
  12. In vitro evaluation of antimicrobial activity of sealers and pastes used in endodontics. J Endod 2000;26(7):391-94.
  13. Effect of CO2 Nd:YAG and ArF excimer lasers on dentin morphology & pulp chamber temperature: An in vitro study. J Endod 2000;26(11):644-48.
  14. Comparative evaluation of antibacterial effects of Nd:YAG laser irradiation in root canals and dentinal tubules. J Endod 2000;26(5):268-70.
  15. A comparison of the morphological changes after Nd:YAG and CO2 Laser irradiation of dentin surfaces. J Endod 2000;26(8):450-53.
  16. Effects of pulsed Nd:YAG Laser irradiation on smear layer at the apical stop and apical leakage after obturation. Int End J 2000;33:266-71.
  17. Bacterial coronal leakage after obturation with three root canal sealers. J Endod 2001;27(1):36-39.
  18. Phase, compositional and morphological changes of human dentin after Nd:YAG Laser treatment. J Endod 2001;27(6):389-93.
  19. Effect of Nd:YAG laser irradiation on the apical leakage of obturated root canals: An electrochemical study. Int End J 2001;34(4):318-21.
  20. Effects of diode laser irradiation on smear layer removal from root canal walls and apical leakage after obturation. Photomed Laser Surgery 2005;23(6):575-81.
  21. Clinical implications of the smear layer in endodontics: A review. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2002;94:658-66.
  22. Ultrastructural changes of human dentin after irradiation by Nd:YAG laser. Lasers Surg Med 2002;30:246-52.
  23. Comparative in vivo analysis of the sealing ability of three endodontic sealers in post-prepared root canals. Int End J 2003;36:857-63.
  24. Morphological alterations of the surfaces of enamel and dentin of deciduous teeth irradiated with Nd:YAG, CO2 and diode lasers. Int J Morphol 2009;27(2):441-46.
  25. The influence of the smear layer on the dentinal tubule penetration depth by three different root canal sealers: An in vitro study. J Endod2004;30(2):100-02.
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