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VOLUME 14 , ISSUE 3 ( May-June, 2013 ) > List of Articles


In vitro Evaluation of the Minimum Bactericidal Concentrations of Different Root-End Filling Materials

Mustafa Murat Koçak, Elif Aybala Oktay, Abdullah Kiliç, Sis Darendeliler Yaman

Citation Information : Koçak MM, Oktay EA, Kiliç A, Yaman SD. In vitro Evaluation of the Minimum Bactericidal Concentrations of Different Root-End Filling Materials. J Contemp Dent Pract 2013; 14 (3):371-374.

DOI: 10.5005/jp-journals-10024-1330

Published Online: 01-08-2013

Copyright Statement:  Copyright © 2013; The Author(s).



The aim of this study was to evaluate the minimum bactericidal concentrations (MBC) of root-end filling materials ProRoot MTA, MTA Angelus and IRM.

Materials and methods

Macrodilution broth method was used. Microorganisms used were: Staphylococcus aureus (ATCC 29213), Enterococcus faecalis (ATCC 29212) and Streptococcus mutans. Serial two-fold dilutions of root-end filling samples were prepared in macrodilution tubes with concentrations ranging from 1/2 to 1/512. The samples dilutions were incubated for 24 hours. After incubation, 0.1 ml of diluted culture was inoculated onto the surface of supplemented sheep blood agar (Merck, Germany) and all plates were incubated at 37°C in aerobic condition for 24 hours. The MBC was defined as the lowest concentration of root-end filling samples where no growth was recorded.


MBC of both mineral trioxide aggregate (MTA) products against S. aureus were recorded as 15.62 mg/ml and for IRM 31.25 mg/ml MBC for both MTA groups against E. faecalis were recorded as 31.25 mg/ml and for IRM 62.5 mg/ml. MBC of all root-end filling samples against S. mutans were recorded as 62.5 mg/ml.


All tested root-end filling materials showed acceptable MBC against S. aureus and E. faecalis.

Clinical significance

All tested materials can be used safely for filling of a root-end cavity.

How to cite this article

Koçak MM, Koçak S, Oktay EA, Kiliç A, Yaman SD. In vitro Evaluation of the Minimum Bactericidal Concentrations of Different Root-End Filling Materials. J Contemp Dent Pract 2013;14(3):371-374.

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