In vitro Evaluation of the Minimum Bactericidal Concentrations of Different Root-End Filling Materials
Mustafa Murat Koçak, Elif Aybala Oktay, Abdullah Kiliç, Sis Darendeliler Yaman
Citation Information :
Koçak MM, Oktay EA, Kiliç A, Yaman SD. In vitro Evaluation of the Minimum Bactericidal Concentrations of Different Root-End Filling Materials. J Contemp Dent Pract 2013; 14 (3):371-374.
The aim of this study was to evaluate the minimum bactericidal concentrations (MBC) of root-end filling materials ProRoot MTA, MTA Angelus and IRM.
Materials and methods
Macrodilution broth method was used. Microorganisms used were: Staphylococcus aureus (ATCC 29213), Enterococcus faecalis (ATCC 29212) and Streptococcus mutans. Serial two-fold dilutions of root-end filling samples were prepared in macrodilution tubes with concentrations ranging from 1/2 to 1/512. The samples dilutions were incubated for 24 hours. After incubation, 0.1 ml of diluted culture was inoculated onto the surface of supplemented sheep blood agar (Merck, Germany) and all plates were incubated at 37°C in aerobic condition for 24 hours. The MBC was defined as the lowest concentration of root-end filling samples where no growth was recorded.
Results
MBC of both mineral trioxide aggregate (MTA) products against S. aureus were recorded as 15.62 mg/ml and for IRM 31.25 mg/ml MBC for both MTA groups against E. faecalis were recorded as 31.25 mg/ml and for IRM 62.5 mg/ml. MBC of all root-end filling samples against S. mutans were recorded as 62.5 mg/ml.
Conclusion
All tested root-end filling materials showed acceptable MBC against S. aureus and E. faecalis.
Clinical significance
All tested materials can be used safely for filling of a root-end cavity.
How to cite this article
Koçak MM, Koçak S, Oktay EA, Kiliç A, Yaman SD. In vitro Evaluation of the Minimum Bactericidal Concentrations of Different Root-End Filling Materials. J Contemp Dent Pract 2013;14(3):371-374.
Microbiological findings and clinical procedures in endodontic cases selected for microbiological investigation. Int Endod J 1997;30:91-95.
Evaluation of the antibacterial activities of calcium hydroxide, chlorhexidine, and camphorated paramonochlorophenol as intracanal medicament. A clinical and laboratory study. J Endod 1997;23:297-300.
In vitro evaluation of the antimicrobial activity of a new resin-based endodontic sealer against endodontic pathogens. J Oral Sci 2008;50:309-313.
In vitro antimicrobial activity of endodontic sealers, MTA-based cements and Portland cement. J Oral Sci 2007;49:41-45.
Antimicrobial and chemical study of MTA, Portland cement, calcium hydroxide paste, Sealapex and Dycal. Braz Dent J 2000;11:3-9.
Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard. 7th ed. Document M7-A7. Wayne, PA: Clinical and Laboratory Standards Institute; 2006.
Comparison of disk diffusion method and broth microdilution method for antifungal susceptibility testing of dermatophytes. Med Mycol 2005;43:61-66.
Comparison between disk diffusion and microdilution methods for determining susceptibility of clinical fungal isolates to caspofungin. J Clin Microbiol 2007;45:3529-3533.
In vitro antimicrobial activity of fill canal, sealapex, mineral trioxide aggregate, Portland cement and EndoRez. Int Endod J 2005;38:539-543.
An in vitro study of the antimicrobial activity of some endodontic medicaments and their bases using an agar well diffusion assay. Aust Dent J 2009;54:141-146.
Enterococcus faecalis– the root canal survivor and star in post-treatment disease. Endod Topics 2003;6:135-159.
Eradication of endodontic infection by instrumentation and irrigation solutions. Endod Topics 2005;10:77-102.
Clinical relevance of bacteriostatic versus bactericidal mechanisms of action in the treatment of Gram-positive bacterial infections. Clin Infect Dis 2004;38:864-870.
Determination of fungicidal activities against yeasts and molds: lessons learned from bactericidal testing and the need for standardization. Clin Microbiol Rev 2004;17:268-280.
Bactericidal effects of antibiotics on slowly growing and nongrowing bacteria. Antimicrob Agents Chemother 1991;35:1824-1828.
Isolation of yeasts and enteric bacteria in root-filled teeth with chronic apical periodontitis. Int Endod J 2001;34:429-434.
Antibacterial effect of two mineral trioxide aggregate (MTA) preparations against Enterococcus faecalis and Streptococcus sanguis in vitro. J Endod 2006;32:1053-1056.
Antibacterial effects of some root-end filling materials. J Endod 1995;21:403-406.
Antibacterial activity of two mineral trioxide aggregate materials in vitro evaluation. Ann Acad Med Stetin 2008;54:147-150.
pH and calcium ion release of 2 root-end filling materials. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2003;95:345-347.
Antibacterial effect of selected root-end filling materials. J Endod 2006;32:345-349.
An in vitro investigation of the antibacterial effect of nisin in root canals and canal wall radicular dentine. Int Endod J 2004;37:664-671.
Nisin and its antimicrobial effect in foods. TAF Prev Med Bull 2007;6:142-147.