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VOLUME 14 , ISSUE 4 ( July-August, 2013 ) > List of Articles


Assessment of Acid Neutralizing Capacity in Cola-based Drinks and Energy Beverages by Artificial Saliva

Marcelo Tomas de Oliveira, Kariny Ramos Felix, Maria Claudia Bressan, Luiz Alberto Kanis

Citation Information : de Oliveira MT, Felix KR, Bressan MC, Kanis LA. Assessment of Acid Neutralizing Capacity in Cola-based Drinks and Energy Beverages by Artificial Saliva. J Contemp Dent Pract 2013; 14 (4):578-581.

DOI: 10.5005/jp-journals-10024-1366

Published Online: 01-10-2013

Copyright Statement:  Copyright © 2013; The Author(s).



To assess the pH of regular and light cola-based drinks and energy drinks, and examine the acid neutralizing capacity by the addition of artificial saliva.

Materials and methods

Ten packages of each product purchased locally at different stores were evaluated. The pH was measured by a pH-electrode calibrated in standard buffer solutions. To assess the pH neutralizing capacity, 1 ml/min of artificial saliva was added until a pH of 5.5 set as a cutoff point was reached in the tested solution. The data were subjected to analysis of variance (ANOVA), at the 0.05 significance level.


The surveyed beverages had an initial acidic pH, ranging between 2.3 and 3.4. The average amount of saliva required to raise the pH of the cola-based drinks above 5.5 ranged between 6.0 and 6.8 ml. Energy drinks used volumes between 11.3 and 12.5 ml; however, it was not possible to achieve a pH of 5.5


According to the methodology used, it was concluded that: (1) All beverages analyzed showed an initial acidic pH. (2) There was no statistical difference between the initial pH level and acid neutralization by the addition of artificial saliva in both regular and light drinks. It was not possible to reach the appropriate pH, set as the cutoff point, for the energy drinks.

How to cite this article

Felix KR, Bressan MC, Kanis LA, De Oliveira MT. Assessment of Acid Neutralizing Capacity in Cola-based Drinks and Energy Beverages by Artificial Saliva. J Contemp Dent Pract 2013;14(4):578-581.

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