Utilization of Dental Pulp DNA as Diagnostic Molecular Marker for Fertility Detection in Men
Hossam A Eid, Tarek H Taha, Manea M Alahmari, Abdullah Awn S Alqarni, Abdulkarim Ali H Alshehri
Citation Information :
Eid HA, Taha TH, Alahmari MM, Alqarni AA, Alshehri AA. Utilization of Dental Pulp DNA as Diagnostic Molecular Marker for Fertility Detection in Men. J Contemp Dent Pract 2013; 14 (4):590-594.
The aim of this study was to use DNA material extracted from human teeth pulp for detection of fertility status of men.
Materials and methods
Twenty extracted premolar teeth of systemic disease free male Saudi individuals (45 years average age) were collected; eight of them were infertile while others were fertile and were used as control group. This information was concealed until the PCR analysis was performed. The results of recorded patient information was matched with the results of the DNA analysis.
Results showed that the gene (sY83) an important gene of AZFa region in Y chromosome is important for male fertility. It was later evident that the infertile patients suffered from azoospermia, and that information is completely matched with our results.
Using DNA extracted from dental pulp can be used successfully in determining fertility status of human which may help in an accurate personal identification specially in extreme circumstances.
How to cite this article
Eid HA, Taha TH, Alahmari MM, Alqarni AAS, Alshehri AAH. Utilization of Dental Pulp DNA as Diagnostic Molecular Marker for Fertility Detection in Men. J Contemp Dent Pract 2013;14(4):590-594.
DNA research in forensic dentistry. Methods Find Exp Clin Pharmacol 2001 Nov;23(9):511-517.
DNA profiling and forensic dentistry: a review of the recent concepts and trends. J Forensic Leg Med 2011 Jul;18(5):191-197.
Modern approaches to the treatment of human infertility through assisted reproduction. PR Health Sci J 1991;10:75-81.
Infertility in men: recent advances and continuing controversies. J Clin Endocrinol Metab 1999;84:3443-3450.
Prognostic value of Y chromosome micro deletion analysis. Hum Reprod 2000;15:1431-1434.
Laboratory guidelines for of Y chromosomal microdeletions. Int J Androl 1999;22:292-299.
Y chromosome and male infertility, Front Biosci 1999;4:1-8.
Mice with Y chromosome deletion and reduced RBM genes on a heterozygous Daz1 null background mimic a human azoospermic factor phenotype. Hum Reprod 1999;14:3023-3029.
Sequence Tagged Site (STS). Analysis of Y-chromosome microdeletions in environmental tobacco smokers (ETS) in Tamil Nadu, India. Advances in Biological Research 2010;4(6):296-300.
The human Y chromosome: overlapping DNA clones spanning the euchromatic region. Science 1992;258:60-66.
The human Y chromosome: a 43 interval map based on naturally occurring deletions. Science 1992;258:52-59.
Towards the molecular localization of the AZF locus: mapping of microdeletions in azoospermic men within 14 subintervals of interval 6 of the human Y chromosome. Human Molecular Genetics 1992;1:29-33.
Diverse spermatogenic defects in humans caused by Y chromosome deletions encompassing a novel RNA-binding protein gene. Nature Genetics 1995;10:383-393.
Human Y chromosome azoospermia factors (AZF) mapped to different subregions in Yq11. Human Molecular Genetics 1996;5:933-943.
Severe oligozoospermia resulting from deletions of azoospermia factor gene on Y chromosome. Lancet 1996;347:1290-1293.
Polymerase chain reaction screening for Y chromosome microdeletions: a first step towards the diagnosis of genetically-determined spermatogenic failure in men. Molecular Human Reproduction 1996;2:775-779.
The DAZ gene family and germ-cell development. In: Goldeberg E, editor. Proceedings of the XVth testis workshop on the testis: from stem cell to sperm function. Serono Symposia Springer, Norwell, MA, USA 2000;213-225.
Prognostic value of Y deletion analysis. Human Reproduction 2001;16:9-12.
Y chromosome microdeletions and alterations of spermatogenesis. Endocrine Reviews 2001;22:226-239.
Prevalence of Y chromosome microdeletions in infertile men who consulted a tertiary care medical centre: the Münster experience. Andrologia 2001;33:27-33.
Deletions within the azoospermia factor subregions of the Y chromosome in Hong Kong Chinese men with severe malefactor infertility: controlled clinical study. Hong Kong Medical Journal 2000;6:143-146.
Prevalence of Y chromosome deletions in a Brazilian population of nonobstructive azoospermic and severely oligozoospermic men. Brazilian J Medical and Biological Research 2003;36:787-793.
Demonstration and semi-quantification of mtDNA from human dentin and its relation to age. Int J Legal Med 1999;112:98-100.
Different dental tissues as source of DNA for human identification in forensic cases. Forensic Sciences 2003;44(3):306-309.
The effect of high temperatures on human teeth and dentures. Int Poster J Dent Oral Med 2004;6:1-4.
Burned and incinerated remains. In: Bowers CM, editor. Manual of Forensic Odontology. Colorado Springs: American Society of Forensic Odontology 1997;16-18.
Thermal stability of direct dental esthetic restorative materials at elevated temperatures. J Forensic Sci 1998431163-1167.
The identification of victim of slaughter of the jail of Sabaneta in forensic odontology. Rev Esp Med Leg 1998;22:50-56.
Direct and indirect restorative materials. J Am Dent Assoc 2003;134:463-472.
Chromosome microdeletions and alterations of spermatogenesis. Endocr Rev 2001;22:226-239.
Unique DNA marker for diabetes and Y chromosome fertility genes in human teeth remains. Egyptian Dental Journal 2012 July;58(3):2409-2415.
Conservation genetics of European brown bear-astudy using excremental PCR of nuclear and mitochondrial sequences. Mol Ecol 1995;4:95-103.