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VOLUME 14 , ISSUE 4 ( July-August, 2013 ) > List of Articles


Influence of Solvents and Composition of Etch-and-Rinse and Self-Etch Adhesive Systems on the Nanoleakage within the Hybrid Layer

Joao Cardoso Ferreira, Patricia Teixeira Pires, Alvaro Ferreira Azevedo, Sofia Arantes Oliveira, Paulo Ribeiro Melo, Mario Jorge Silva

Citation Information : Ferreira JC, Pires PT, Azevedo AF, Oliveira SA, Melo PR, Silva MJ. Influence of Solvents and Composition of Etch-and-Rinse and Self-Etch Adhesive Systems on the Nanoleakage within the Hybrid Layer. J Contemp Dent Pract 2013; 14 (4):691-699.

DOI: 10.5005/jp-journals-10024-1386

Published Online: 01-12-2013

Copyright Statement:  Copyright © 2013; The Author(s).



The goal of this study was to evaluate nanoleakage within the hybrid layer yielded by etch-and-rinse and self-etch adhesive systems, with different solvents and compositions.

Materials and methods

Four adhesives were applied onto 20 human dentin disks: group A: Adper Scotchbond 1XT™ (3M ESPE), group B: One Coat Bond® (Coltène Whaledent), group C: AdheSE® (Ivoclar Vivadent) and group D: Xeno-V® (Dentsply). The samples were immersed in aqueous ammoniacal silver nitrate for 24 hour, prepared and observed under field-emission scanning electron microscopy with backscattered electrons. Microphotographs were scanned and data were processed. The mean value and standard deviation were calculated. KruskalWallis and Mann-Whitney tests were used (p < 0.05).


All the adhesives showed nanoleakage within the hybrid layer: Adper Scotchbond 1XT™ (218.5 μm ± 52.6 μm), One Coat Bond® (139.6 μm ± 79.0 μm), AdheSE® (92.7 μm ± 64.8 μm) and Xeno-V® (251.0 μm ± 85.2 μm). AdheSE® yielded less nanoleakage than Adper Scotchbond-1XT™ (p = 0.003) and than Xeno-V® (p = 0.007). No other statistically significant differences were detected.


Two-step self-etch adhesive system (AdheSE®) might contribute for lower nanoleakage deposition and thus better performance in dentin adhesion.

Clinical significance

The two-step self-etch adhesive system showed the lowest nanoleakage deposition compared with the other adhesive systems evaluated, which seems to indicate a better behavior when a restoration is performed in dentin and possibly can lead to a durable adhesion along time.

How to cite this article

Ferreira JC, Pires PT, Azevedo AF, Oliveira SA, Melo PR, Silva MJ. Influence of Solvents and Composition of Etch-and-Rinse and Self-Etch Adhesive Systems on the Nanoleakage within the Hybrid Layer. J Contemp Dent Pract 2013;14(4):691-699.

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