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VOLUME 14 , ISSUE 6 ( November-December, 2013 ) > List of Articles


Determination of Inner Implant's Volumes: A Pilot Study for Microleakage Quantification by Stereomicroscopy and Spectrophotometry

A Berberi, G Tehini, Z Tabaja, A Kobaissi, K Hamze, K Rifai, M Ezzedine, B Badran, A Chokr

Citation Information : Berberi A, Tehini G, Tabaja Z, Kobaissi A, Hamze K, Rifai K, Ezzedine M, Badran B, Chokr A. Determination of Inner Implant's Volumes: A Pilot Study for Microleakage Quantification by Stereomicroscopy and Spectrophotometry. J Contemp Dent Pract 2013; 14 (6):1122-1130.

DOI: 10.5005/jp-journals-10024-1462

Published Online: 01-06-2014

Copyright Statement:  Copyright © 2013; The Author(s).



Microleakage quantification of fluids and microorganisms through the connections of different implant parts seems to be sparse. Moreover, no data exist regarding the determination of the volumes of inner parts of dental implant systems.

This study aims to determine the volumes of inner parts of three dental implant systems with the same interface and to evaluate the microleakage phenomenon.

Materials and methods

Three implant system sets (Euroteknika ®, Astra Tech® and Implantium®) were used in this study. Implants were inoculated with safranin, brain heart infusion and distilled water. After inoculation and assembly of the different parts, different inner volumes (V1, V2, V3, V4, V5 and V6) were measured and, the surfaces of the micro gaps were observed through a stereomicroscope. Implants containing safranin were immersed in vials containing distilled water. Samples then were taken to determine optical density using a spectrophotometer.


Regardless the used substance, volumes of the 3-implant systems are different. Although volumes V1, V2, V3 and V5 appeared to be constant within the same system regardless the used substance, volumes V4 and V6 were not.


The determination of the volumes and the evaluation of leaked substance using stereomicroscopic and spectrophotometric methods showed the accuracy of these methods and the importance of their use in the study of microleakage.

Clinical significance

Leakage is an important factor for chronic inflammatory infiltration and marginal bone resorption. Studies have shown fluid and bacterial leakage into abutmentimplant (A-I) assemblies of certain implants with ‘closely locked’ abutments and the creation of a constant bacterial reservoir in the empty space found between the implant and the abutment.

How to cite this article

Berberi A, Tehini G, Tabaja Z, Kobaissi A, Hamze K, Rifai K, Ezzedine M, Badran B, Chokr A. Determination of Inner Implant's Volumes: A Pilot Study for Microleakage Quantification by Stereomicroscopy and Spectrophotometry. J Contemp Dent Pract 2013;14(6):1122-1130.

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