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VOLUME 15 , ISSUE 2 ( March-April, 2014 ) > List of Articles

RESEARCH ARTICLE

In situ Assessment of Effects of the Bromide- and Fluoride-incorporating Adhesive Systems on Biofilm and Secondary Caries

Suyane Maria Luna Cruz de Vasconcelos, Mary Anne Sampaio de Melo, Joao Paulo Marques Saraiva Wenceslau, Iriana Carla Junqueira Zanin, Haroldo Cesar Pinheiro Beltrao, Carlos Augusto Oliveira Fernandes, Paulo Cesar de Almeida, Lidiany Karla Azevedo Rodrigues

Citation Information : de Vasconcelos SM, de Melo MA, Wenceslau JP, Zanin IC, Beltrao HC, Fernandes CA, de Almeida PC, Rodrigues LK. In situ Assessment of Effects of the Bromide- and Fluoride-incorporating Adhesive Systems on Biofilm and Secondary Caries. J Contemp Dent Pract 2014; 15 (2):142-148.

DOI: 10.5005/jp-journals-10024-1504

Published Online: 01-10-2014

Copyright Statement:  Copyright © 2014; The Author(s).


Abstract

Aim

This in situ study assessed the effects of adhesive systems containing or not fluoride and/or the antibacterial monomer 12-methacryloyloxydodecylpyridinium bromide (MDPB) on the microbiological composition of dental biofilm and enamel demineralization.

Materials and methods

During two phases of 14 days, ten volunteers wore intraoral palatal appliances containing two slabs of human enamel according to a double-blind, crossover design. The slabs were randomly restored using a composite resin and one of the following adhesive systems: All-Bond SETM (self-etch, fluoride/MDPB free adhesive, AB) and Clearfil Protect Bond (self-etch containing fluoride and MDPB adhesive, CB). The biofilm formed on the slabs was analyzed with regard to total and mutans streptococci and lactobacilli counts. Demineralization represented by integrated area of hardness × lesion depth Delta S (ƒ¢S) was determined on enamel by analysis of cross-sectional microhardness, at 20 and 70 ƒÊm from the restoration margin. Data were analyzed by ANOVA.

Results

No statistically significant difference was found either in enamel demineralization or in the microbiological composition of dental biofilm.

Conclusion

All adhesive systems containing or not fluoride and/or MDPB tested were unable to inhibit secondary caries in the in situ model used in the present research.

How to cite this article

de Vasconcelos SMLC, de Melo MAS, Wenceslau JPMS, Zanin ICJ, Beltrao HCP, Fernandes CAO, de Almeida PC, Rodrigues LKA. In situ Assessment of Effects of the Bromide- and Fluoride-Incorporating Adhesive Systems on Biofilm and Secondary Caries. J Contemp Dent Pract 2014;15(2):142-148.

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