A Quantitative Analysis of Mast Cells in Inflammatory Periapical and Gingival Lesions
Kavita Rao, NS Priya, K Uma, HS Umadevi, T Smitha
Citation Information :
Rao K, Priya N, Uma K, Umadevi H, Smitha T. A Quantitative Analysis of Mast Cells in Inflammatory Periapical and Gingival Lesions. J Contemp Dent Pract 2014; 15 (3):300-305.
The aim of the study was to quantify the presence of mast cells in various inflammatory lesions like periapical granuloma, periapical cyst, inflammatory gingival hyperplasia and pyogenic granuloma. Mast cell degranulation and association with lymphocytes were also recorded in an attempt to understand the role of mast cells in the pathogenesis of these inflammatory lesions.
Materials and methods
The quantification of mast cells was done on toluidine blue stained sections of all the four groups of lesions, using the image analyzer software, Image-Pro-Express (Media Cybernetics, USA).
Results
An increased number of mast cells in various inflammatory lesions with a significant difference between the four groups were noted. Mast cell number tended to be greater in the lesions present in the anterior region of the mouth than in the posterior region of the oral cavity. The mean mast cell number decreased with the increasing age which was directly correlated with the age of the patients. Mast cell site, distribution, degranulation and its association with fibroblasts, lymphocytes and blood vessels were noted.
Conclusion
The location of mast cells in different areas, their association with lymphocytes, fibroblasts, endothelial cells, and the phenomenon of degranulation helps to appreciate the release of various mediators and multiple interactions among these cells, leading to increased vascular permeability, angiogenic response, collagen synthesis, regulation of inflammation, bone resorption, and extracellular matrix destruction, thus contributing to the pathogenesis of these inflammatory lesions.
How to cite this article
Sheethal HS, Uma K, Rao K, Priya NS, Umadevi HS, Smitha T. A Quantitative Analysis of Mast Cells in Inflammatory Periapical and Gingival Lesions. J Contemp Dent Pract 2014;15(3):300-305.
Mast cell and fibrosis. Arch Dermatol 1987;123(2):191-193.
The mast cell, its role in health and disease. Proceedings of an international symposium, Davos, Switzerland. England: Pitman Medical Publishing Co. Ltd; 1979. p. 9-20.
Relationship between mast cell degranulation and inflammation in the oral cavity. Crit Rev Oral Biol 2003;14(3):188-198.
Immunology. 7th ed. China: Mosby Elsevier; 2006. p. 432.
Comparative immunohistochemical and quantitative analysis of inflammatory cells in symptomatic and asymptomatic chronic periapical lesions. Vojnosanit Pregl 2008;65(6):435-440.
Clinical periodontology (Revised edition). New Delhi: Arya (Medi) Publishing House; 2005. p. 136-137.
Study of mast cells in periodontal disease. J Oral Maxillofac Pathol 2006;10(2):64-68.
Importance of mast cells in human periapical inflammatory lesions. J Endod 2004;30(12):855-859.
Mast cells of the human gingiva. J Perio Res 2006;1(4):46-55.
Mast cells — a role in periodontal diseases. J Clin Periodontol 2004;31(6):413-419.
A toluidine blue method for demonstrating mast cells. J Histotechnology 1981;2:102-103.
Cells infiltrating human periapical inflammatory lesions. J Oral Pathol 1986;15(10):544-546.
Mast cells in human odontogenic cysts. J Oral Pathol Med 1989;18(5):274-278.
Incidence of mast cells in oral inflammatory lesions: a pilot study. J Oral Maxillofac Pathol 2005;9(1):12-15.
Mast cell ‘densities’ in vascular proliferations: a preliminary study of pyogenic granuloma, portwine stain cavernous hemangioma, cherry angioma, Kaposi's sarcoma and malignant hemangioendothelioma. J Dermatol 1999;26(9):577-586.
Quantification of mast cells in different stages of human periodontal disease. Oral Dis 2005;11(4):249-254.
Comparative immunohistochemical study of the presence of mast cells in apical granulomas and periapical cysts: possible role of mast cells in the course of human periapical lesions. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2004;97(1):59-63.
Mast cells in gingival inflammations, J Oral Pathol Med 1989;16(3):481-483.
Phagocytosis of mast cell granules by fibroblasts of the human gingiva. Virchows Arch 1985;406(2):197-201.
Relationship between mast cell degranulation and inflammation in the oral cavity. J Oral Pathol Med 1995;24(6):266-272.
Cellular composition of periapical granulomas and its function: Histological, immunohistochemical and electron microscopic study, Czech Med 1989;12(4):193-215.
Increase of mast cells and tumor angiogenesis in oral squamous cell carcinoma. J Oral Path Med 2003;32(4):195-199.
Mast cells are increased in leukoplakia, oral submucous fibrosis, oral lichen planus and oral squamous cell carcinoma. J Oral Maxillofac Pathol 2007;11(1):18-22.
The production of collagen and the activity of mast-cell chymase increase in human skin after irradiation therapy. Exp Dermatol 2004;13(6):364-371.
Effect of histamine on proliferation of normal human adult lung fibroblasts. Thorax 1988;43(7):552-528.
Effects of histamine on collagen by cultured fibroblasts derived from guinea pig skin Arch Oral Biol 1984;277(1):60-64.
Mast cell density in oral submucous fibrosis, J Ind Dent Assoc 1977;49:187-191.
Investigation of mast cells in human gingiva following low intensity laser irradiation. Photomed Las Surg 2008;26(4):315-321.
