The Journal of Contemporary Dental Practice

Register      Login



Volume / Issue

Online First

Related articles

VOLUME 15 , ISSUE 5 ( September-October, 2014 ) > List of Articles


Clinical Trial of an Experimental Cleaning Solution: Antibiofilm Effect and Integrity of a Silicone-based Denture Liner

Antonio de Luna Malheiros Segundo, Marina Xavier Pisani, Cássio do Nascimento, Raphael Freitas Souza, Helena de Freitas Oliveira Paranhos, Cláudia Helena Silva-Lovato

Citation Information : de Luna Malheiros Segundo A, Pisani MX, Nascimento CD, Souza RF, de Freitas Oliveira Paranhos H, Silva-Lovato CH. Clinical Trial of an Experimental Cleaning Solution: Antibiofilm Effect and Integrity of a Silicone-based Denture Liner. J Contemp Dent Pract 2014; 15 (5):534-542.

DOI: 10.5005/jp-journals-10024-1575

Published Online: 01-02-2015

Copyright Statement:  Copyright © 2014; The Author(s).



This study evaluated the antibiofilm effect of an experimental solution of 2% Ricinus communis (R. communis) on a silicone-based denture liner by means of a randomized clinical trial, as well as the integrity of such liner following a cleansing regimen with such solution.

Materials and methods

About 30 complete denture wearers had their lower dentures relined with a silicone-based denture liner and randomly allocated to cleanse their dentures by means of: (A) a specific toothbrush for complete dentures and dentifrice; (B) soaking in an experimental 2% R. communis solution; and (C) association of A and B. Considered outcomes were biofilm coverage area (%), microbial counts by means of the deoxyribonucleic acid (DNA) checkerboard hybridization technique and physical integrity of the soft liner, assessed during a time interval of 60 days. Mean group values were compared by analysis of variance (ANOVA) and Tukey tests or generalized linear method (α = 0.05).


The mechanical method presented the lowest biofilm percentage (1.45 ± 1.03) if compared to the chemical method (2.96 ± 1.98) and the associated one (2.71 ± 1.76). After 60 days (3.37 ± 2.04), biofilm accumulation was higher than at 15 days (1.28 ± 0.77) and 30 days (2.46 ± 1.54). The denture liner was less deteriorated and kept its physical integrity when the mechanical method was applied. The chemical method presented higher effectiveness against microorganisms, including some Candida species.


The 2% R. communis solution presented stronger antimicrobial capacity than brushing on a silicone-based denture liner after immersion. However, it was not superior to the mechanical method in preserving the physical integrity of the material and in biofilm removal.

Clinical significance

Soft denture liners hygiene is a very important issue and not conclusive in the literature. The experimental solution of 2% R. communis evaluated presented promising antimicrobial potential and should be more explored to be recommended as cleanser.

How to cite this article

de Luna Malheiros Segundo A, Pisani MX, do Nascimento C, Souza RF, de Freitas Oliveira Paranhos H, Silva-Lovato CH. Clinical Trial of an Experimental Cleaning Solution: Antibiofilm Effect and Integrity of a Silicone-based Denture Liner. J Contemp Dent Pract 2014;15(5):534-542.

PDF Share
  1. Soft lining materials in prosthetic dentistry: a review. Int J Prosthodont 1990 Sep-Oct;3(5):477-483.
  2. Clinical effects of acrylic resilient denture liners applied to mandibular complete dentures on the alveolar ridge. J Oral Rehabil 2007 Nov;34(11):862-869.
  3. Electromyography of masticatory muscles after denture relining with soft and hard denture liners. J Oral Sci 2013 Sep;55(3):217-224.
  4. Changes in surface roughness and colour stability of soft denture lining materials caused by denture cleansers. J Oral Rehabil 2003 Feb;30(2):125-130.
  5. Examination of denture-cleaning methods based on the quantity of microorganisms adhering to a denture. Gerodontology 2012 Jun;29(2):e259-266.
  6. A review of in vitro and in vivo methods to evaluate the efficacy of denture cleansers. Int J Prosthodont 1999 Mar-Apr;12(2):153.
  7. Effects of the domestic use of a disclosing solution on the denture biofilm: a preliminary study. J Oral Rehabil 2009 Jul;36(7):491-497.
  8. Oral hygiene habits, denture cleanliness, presence of yeasts and stomatitis in elderly people. J Oral Rehabil 2002 Mar;29(3):300-304.
  9. Morphologic and Morphometric analysis of the root canal apical third cleaning after biomechanical preparation using 3.3% Ricinus communis detergent and 1% NaOCl irrigating solutions. J Appl Oral Sci 2006 Jun;14(3):178-182.
  10. Castor oil: antimicrobial activity of detergent derived from ricinoleic acid. J Dent Res 1999;78(2):344.
  11. The effect of experimental solution Ricinus communis on acrylic resin acrylic properties. Mater Res 2010;13:369-373.
  12. Evaluation of experimental cleanser solution of Ricinus communis: effect on soft denture liner properties. Gerodontology 2012 Jun;29(2):e179-185.
  13. Effect of chlorhexidine on denture biofilm accumulation. J Prosthodont 2012 Jan;21(1):2-6.
  14. Comparative study of methods for the quantification of biofilm on complete dentures. Braz Oral Res 2004;18(3):215-223.
  15. Evaluation of a computerized method for denture biofilm quantification: interexaminer reproducibility. J Prosthodont 2009 Jun;18(4):332-336.
  16. Comparative analysis of biofilm levels in complete upper and lower dentures after brushing associated with specific denture paste and neutral soap. Gerodontology. 2007 Dec;24(4):217-223.
  17. Checkerboard DNA-DNA hybridization. Biotechniques 1994 Oct;17(4):788-792.
  18. Biofilms in the edentulous oral cavity. J Prosthodont 2008 Jul;17(5):348-356.
  19. Subgingival microbiota in healthy, wellmaintained elder and periodontitis subjects. J Clin Periodontol 1998 May;25(5):346-353.
  20. Alternative method for direct DNA probe labeling and detection using the checkerboard hybridization format. J Clin Microbiol 2010 Aug;48(8):3039-3040.
  21. Bacterial involvement in denture-induced stomatitis. J Dent Res 1988;67:1246-1250.
  22. Growth inhibition of oral bacteria related to denture stomatitis by anticandidal chalcones. Aust Dent J 1997 Oct;42(5):343-346.
  23. Putative periodontal pathogens colonizing oral mucous membranes in denture-wearing subjects with a past history of periodontitis. J Clin Periodontol 1995 Nov;22(11):854-859.
  24. Biofilm formation of Candida albicans on the surface of a soft denturelining material. Gerodontology 2009 Sep;26(3):210-203.
  25. Characterization of the adhesion of soft lining materials to poli (methyl methacrylate). J Dent Res 1982 Aug;61(8):1002-1005.
  26. A six-year retrospective study of Molloplast-B-lined dentures. Part II: Liner serviceability. J Prosthet Dent 1983 Oct;50(4):459-465.
  27. The effect of aging by thermal cycling and mechanical brushing on resilient denture liner hardness and roughness. J Prosthodont 2008 Jun;17(4):318-322.
  28. Influence of toothbrushing on surface roughness of soft denture liners: an in vitro study. RPG Revista da Pos-Graduacao 2004;11(2):125-129.
  29. Observations on long-term use of a soft-lining material for mandibular complete dentures. J Prosthet Dent 1994 Oct;72(4):385-392.
  30. ystrand F, Saxegaard E, Dogan A, Bek B, Ruyter IE. A prospective study on the clinical performance of polysiloxane soft liners: one-year results. Dent Mater J 2008 May;27(3):440-447.
  31. Presence of microorganisms on the fitting denture complete surface: study in vivo. J Oral Rehabil 2000 Aug;27(8):708-713.
  32. Evaluation of the antimicrobial activity of three irrigation solutions in teeth with pulpal necrosis. Br Dent J 1999;10(1):15-21.
  33. The effect of Streptococcus mutans and Candida glabrata on Candida albicans biofilms formed on different surfaces. Arch Oral Biol 2008 Aug;53(8):755-764.
  34. Flocculation, adhesion and biofilm formation in yeasts. Mol Microbiol 2006 Apr;60(1):5-15.
  35. Surface roughness and adherence of Candida albicans on soft lining materials as influenced by accelerated aging. J Contemp Dent Pract 2007 Jul;8(5):18-25.
  36. Colonization and penetration of denture soft lining materials by Candida albicans. Dent Mater 2004 Feb;20(2):167-175.
  37. Tratado de Periodontia Clínica e Implantologia Oral. Rio de Janeiro: Guanabara-Koogan, 2005. 1013.
  38. Biofilm formation of Candida albicans on the surfaces of deteriorated soft denture lining materials caused by denture cleansers in vitro. J Oral Rehabil 2003 Mar;30(3):243-250.
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.