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VOLUME 24 , ISSUE 3 ( March, 2023 ) > List of Articles


A SEM Evaluation of the Permeability of Different Desensitizing Methods on Occlusion of Dentinal Tubules: An In-vitro Study

Basil Joy, Niva Mahapatra, Joy Mathew, Feby Kuriakose, Kumari Lucy Bhola

Keywords : Dentinal hypersensitivity, Dentinal tubules, Desensitizing methods scanning electron microscope

Citation Information : Joy B, Mahapatra N, Mathew J, Kuriakose F, Bhola KL. A SEM Evaluation of the Permeability of Different Desensitizing Methods on Occlusion of Dentinal Tubules: An In-vitro Study. J Contemp Dent Pract 2023; 24 (3):153-156.

DOI: 10.5005/jp-journals-10024-3459

License: CC BY-NC 4.0

Published Online: 25-05-2023

Copyright Statement:  Copyright © 2023; The Author(s).


Aim: The purpose of the current research was to assess the permeability of three various desensitizing techniques on dentinal tubule occlusion using a scanning electron microscope (SEM). Materials and methods: For this research, 100 human sound premolar teeth that were extracted for orthodontic purposes were gathered. With the aid of a water-cooled diamond saw, the teeth were divided in a mesiodistal (vertically) orientation. A sectioned sample (5 mm long by 5 mm wide by 3.5 mm deep), including the cervical region, was taken from each buccal side. To fully open the dentinal tubules, these samples were then kept in 17% ethylenediaminetetraacetic acid (EDTA) for 40 minutes. The samples were divided into four groups (n = 25), each receiving the following dentin surface treatments: Group I: Control, Group II: Samples received NaF 5% varnish treatment, Group III: Samples received diode laser treatment, and Group IV: Samples received CPP–ACP treatment. An SEM was used to inspect each specimen at a magnification of about × 3000 and photomicrographs was assessed. Results: The maximum occlusion of dentinal tubules was found in samples were treated with Diode laser (2.96 ± 0.14) followed by samples treated with NaF 5% varnish (3.38 ± 0.10), samples were treated with CPP–ACP (3.42 ± 0.06) and control group (4.26 ± 0.19). There was a statistically significant difference found between the groups. Conclusion: In conclusion, all three desensitizing methods used in the present study were successful in the occlusion of dentin tubules. But the application of the Diode laser was effective in the reduction of dentin permeability compared to NaF 5% varnish and CPP–ACP. Clinical significance: Dentin hypersensitivity (DH) is characterized by a brief period of intense discomfort. One approach to managing DH is to obstruct dentin tubules in order to decrease dentin permeability. There are many substances that can reduce hypersensitivity, but the finest commercially available substance for treating the condition by occluding the tubes should be acknowledged.

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