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VOLUME 15 , ISSUE 5 ( September-October, 2014 ) > List of Articles

RESEARCH ARTICLE

Measurement of Nitrite and Nitrate in Saliva of Children with Different Caries Activity

Durdi Qujeq, Maryam Rabiee, Mahtab Hamzeh

Citation Information : Qujeq D, Rabiee M, Hamzeh M. Measurement of Nitrite and Nitrate in Saliva of Children with Different Caries Activity. J Contemp Dent Pract 2014; 15 (5):623-625.

DOI: 10.5005/jp-journals-10024-1590

Published Online: 01-10-2014

Copyright Statement:  Copyright © 2014; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Objective of the study

Recently, there has been growing interest in the role of salivary nitrate and nitrite in caries protection. Nitrate is a natural compound found in fruits and vegetables and when secreted in saliva, is reduced to nitrite through bacterial respiration and subsequently reduced to nitric oxide in acidic condition. Nitric oxide takes part in oral non-specific immune system and prevents bacterial growth. The aim of present study was to determine the concentration of nitrite and nitrate in saliva of children with different caries activity.

Materials and methods

Ninety three children, 4 to 6 years old, enrolled in this case-control study and were divided into 3 groups; 31 caries free children, 31 with 5 <DFS ≤ 10 and 31 with DFS >10. Unstimulated saliva was collected and stored in 4°C. Measurement of nitrate and nitrite concentration was performed using Griess reaction. Data were analyzed by T-test, Chi-square, ANOVA and multiple comparisons using SPSS 18. p < 0.05 was considered significant.

Results

Mean value of DFS in the first, second and third were 0, 7.12 and 12.61 respectively. Mean value of nitrite and nitrate in the third group was significantly higher than two others (p < 0.05), but the difference between first and second group was not significant.

Conclusion

Increase in DFS was associated by increase in salivary nitrite and nitrate concentration.

Clinical significance

High concentration of nitrate and nitrite is not enough for caries prevention.

How to cite this article

Ghasempour M, Qujeq D, Rabiee M, Hamzeh M. Measurement of Nitrite and Nitrate in Saliva of Children with Different Caries Activity. J Contemp Dent Pract 2014;15(5):623-625.


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  1. Pediatric Dentistry Infancy Through Adolescence. 4th ed. Elsevier Inc. St. Louis, Missouri 2005;199-204.
  2. Dentistry of child and adolescent. 8th ed. Blackwell Munksgaard 2004;215-232.
  3. Resemblance of salivary protein profiles between children whit early childhood caries and carie-free controls. Eur J Oral Sci 2009 Aug;117(4):369-373.
  4. Saliva and dental caries. J Dent clin of North Am 1999 Oct;43(4):579-597.
  5. Protective effect of salivary nitrate and microbial nitrate reductase activity against caries. Eur J Oral Sci 2004 Oct;112(5):424-428.
  6. Salivary nitrate–an ecological factor in reducing oral acidity. Oral Mic Robiol Immunol 2007 Feb;22(1):67-71.
  7. Unraveling the chemical identity of meat pigments. Crit Rev food Sci Nutr 1997 Oct;37(6):561-589.
  8. Evidence that ingested nitrate is beneficial to health. J Food Port 2002 May;65(5):872-875.
  9. The role of nitric oxide in saliva in reduction of caries. ACTA FAC MED NAISS 2008;25(2):93-95.
  10. Effect of nitrae on the growth and acidogenicity of Streptococcus mutans. J Dent 2002 Sep-Nov;30(7-8):325-331.
  11. Dynamic aspect of reactive oxygen and nitric oxide in oral cavity. J Chin Biochem Nutr 2008 Jan; 42:8-13.
  12. Antibacterial effects of nitrite in urine. Thesis, Karolinska Institute, Stockholm, Sweden. 2005.
  13. Salivary nitric oxide as biomarker of caries risk in children. Acta stomatol croat 2009;43(1):39-44.
  14. Antimicrobial effect of acidified nitrate and nitrite on six common oral pathogens in vitro. Chin Med J 2006 Nov 20;119(22):1904-1909.
  15. Nitric oxid and macrophage function. Annu Rev Immunol 1997;15:323-350.
  16. Nitric oxide is released resulting in regenerating liver after partial hepatectomy. Hepatology 1995 Mar;21(3):776-786.
  17. Nitric oxide concentration in saliva in relation to caries. J Isfahan Dent School 2007;3(2):71-75.
  18. Salivary contribution to exhaled nitric oxide. Eur Respir J 1999 Feb;13(2):327-333.
  19. Nitric oxide concentration in saliva and dental plaque in relation to caries experience and oral hygiene. Caries Res 2005 Mar-Apr;39(2):130-133.
  20. Oral nitric oxide during plaque deposition. Eur J Clin Invest 2001 Oct;31(10):876-879.
  21. Nitrite reductase in Streptococcus mutans plays a critical role in the survival of this pathogen in oral cavity. Oral Microbial Immunol 2007 Dec;22(6):384-389.
  22. Evaluation of nitric oxide in saliva of children with rampant caries and early childhood caries: a comparative study. J Clin Pediater Dent 2008 Summer;32(4):283-286.
  23. Longitudinal analysis of the association of human salivary antimicrobial agent with caries increment and cariogenic microorganisms: a 2-year cohort study. J Dent Res 1998 Jan; 77(1):73-80.
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