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VOLUME 15 , ISSUE 6 ( November-December, 2014 ) > List of Articles

RESEARCH ARTICLE

Analysis of the Proliferative Potential of Odontogenic Epithelial Cells of Pericoronal Follicles

Natalia Cimadon, Isabel Silva Lauxen, Vinicius Coelho Carrard, Manoel Sant'Ana Filho, Pantelis Varvaki Rados, Márcia Gaiger Oliveira

Citation Information : Cimadon N, Lauxen IS, Carrard VC, Filho MS, Rados PV, Oliveira MG. Analysis of the Proliferative Potential of Odontogenic Epithelial Cells of Pericoronal Follicles. J Contemp Dent Pract 2014; 15 (6):761-765.

DOI: 10.5005/jp-journals-10024-1613

Published Online: 01-04-2015

Copyright Statement:  Copyright © 2014; The Author(s).


Abstract

Aim

To evaluate the proliferative potential and the cell proliferation rate of odontogenic epithelial cells.

Materials and methods

Forty-two cases of pericoronal follicles of impacted third molars were submitted to silver impregnation technique for quantification of argyrophilic nucleolar organizer regions (AgNOR) and immunohistochemical staining for EGFR and Ki-67. For AgNOR quantification, the mean number of active nucleolar organizer regions per nucleus (mAgNOR) and the percentage of cells with 1, 2, 3 and 4 or more AgNORs per nucleus (pAgNOR) were quantified. Ki-67 immunolabeling was quantified, whereas for EGFR, a descriptive analysis of staining patterns (membrane, cytoplasm or membrane + cytoplasm positivity) was performed. We evaluated the reduced epithelium of the enamel organ and/or islands of odontogenic epithelium present in the entire connective tissue.

Results

mAgNOR were 1.43 (1.0-2.42) and were significantly different among pericoronary follicles from upper and lower teeth (p = 0.041). Immunostaining of Ki-67 was negative in all cases. EGFR immunolabeling was found mainly in the cytoplasm and was more intense in islands and cords when compared to reduced epithelium of the enamel organ.

Conclusion

Odontogenic epithelial cells of some pericoronal follicles have proliferative potential, suggesting their association with the development of odontogenic lesions.

Clinical significance

The authors suggest that nonerupted, especially of the lower teeth, should be monitored and if necessary removed.

How to cite this article

Cimadon N, Lauxen IS, Carrard VC, Filho MSA, Rados PV, Oliveira MG. Analysis of the Proliferative Potential of Odontogenic Epithelial Cells of Pericoronal Follicles. J Contemp Dent Pract 2014;15(6):761-765.


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