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VOLUME 16 , ISSUE 1 ( January, 2015 ) > List of Articles


Comparative Effects of Photodynamic Therapy mediated by Curcumin on Standard and Clinical Isolate of Streptococcus mutans

Marco Aurelio Paschoal, Caroline C Tonon, Marilia Correia, Denise MP Spolidório, Vanderlei S Bagnato, Juçaíra SM Giusti, Lourdes Santos-Pinto

Citation Information : Paschoal MA, Tonon CC, Correia M, Spolidório DM, Bagnato VS, Giusti JS, Santos-Pinto L. Comparative Effects of Photodynamic Therapy mediated by Curcumin on Standard and Clinical Isolate of Streptococcus mutans. J Contemp Dent Pract 2015; 16 (1):1-6.

DOI: 10.5005/jp-journals-10024-1626

Published Online: 01-05-2015

Copyright Statement:  Copyright © 2015; The Author(s).



The aim of this study was investigate the effect of photodynamic therapy (PDT) using curcumin (C) as a photosensitizing agent irradiated with an LED (L) in the blue wavelength as a light source on a standard and clinical isolate of Streptococcus mutans (S. mutans) in a planktonic suspension model.

Materials and methods

Suspensions of both strains were divided into 4 groups as follows: absence of C and L (control group: C–L–), with C and without L (C group: C+L–), absence of C with L (L group: C–L+) and presence of C and L (PDT group: C+L+). Three different concentrations of curcumin (0.75 mg/ml, 1.5 mg/ml and 3 mg/ml) and three light fluences of studied light source (24, 48 and 72 J cm–2) were tested. Aliquots of each studied group was plated in BHI agar and submitted to colony forming units counting (CFU/ml) and the data transformed into logarithmical scale.


A high photoinactivation rate of more than 70% was verified to standard S. mutans strain submitted to PDT whereas the clinical isolate showed a lower sensitivity to all the associations of curcumin and LED. A slight bacterial reduction was verified to C+L– and C–L+, demonstrating no toxic effects to the isolated application of light and photosensitizer to both S. mutans strains tested.


Photodynamic therapy using a combination of curcumin and blue LED presented a substantial antimicrobial effect on S. mutans standard strain in a planktonic suspension model with a less pronounced effect on its clinical isolate counterparts due to resistance to this alternative approach.

Clinical significance

Alternative antimicrobial approaches, as photodynamic therapy, should be encouraged due to optimal results against cariogenic bacteria aiming to prevent or treat dental caries.

How to cite this article

Tonon CC, Paschoal MA, Correia M, Spolidório DMP, Bagnato VS, Giusti JSM, Santos-Pinto L. Comparative Effects of Photodynamic Therapy mediated by Curcumin on Standard and Clinical Isolate of Streptococcus mutans. J Contemp Dent Pract 2015;16(1):1-6.

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