The Journal of Contemporary Dental Practice

Register      Login

SEARCH WITHIN CONTENT

FIND ARTICLE

Volume / Issue

Online First

Archive
Related articles

VOLUME 16 , ISSUE 2 ( February, 2015 ) > List of Articles

RESEARCH ARTICLE

Titanium Surface Roughing Treatments contribute to Higher Interaction with Salivary Proteins MG2 and Lactoferrin

Yuri Wanderley Cavalcanti, Rodrigo Villamarim Soares, Marina Araújo Leite Assis, Elton Gonçalves Zenóbio, Francisco Mauro da Silva Girundi

Citation Information : Cavalcanti YW, Soares RV, Assis MA, Zenóbio EG, da Silva Girundi FM. Titanium Surface Roughing Treatments contribute to Higher Interaction with Salivary Proteins MG2 and Lactoferrin. J Contemp Dent Pract 2015; 16 (2):141-146.

DOI: 10.5005/jp-journals-10024-1651

Published Online: 01-07-2015

Copyright Statement:  Copyright © 2015; The Author(s).


Abstract

Purpose

This study aimed to evaluate the interactions between salivary proteins and titanium disks with different surface treatments.

Materials and methods

Machined titanium disks (n = 48) were divided into four experimental groups (n = 12), according to their surface treatments: surface polishing (SP); acid etching (A); spot-blasting plus acid etching (SB-A); spot-blasting followed by acid etching and nano-functionalization (SB-A-NF). Titanium surfaces were characterized by surface roughness and scanning electron microscopy (SEM). Specimens were incubated with human saliva extracted from submandibular and sublingual glands. Total salivary protein adsorbed to titanium was quantified and samples were submitted to western blotting for mucin glycoprotein 2 (MG2) and lactoferrin identification.

Results

Surface roughness was statistically higher for SB-A and SB-A-NF groups. Scanning electron microscopy images confirmed that titanium surface treatments increased surface roughness with higher number of porous and scratches for SB-A and SB-A-NF groups. Total protein adsorption was significantly higher for SB-A and SB-A-NF groups (p < 0.05), which also presented higher interactions with MG2 and lactoferrin proteins.

Conclusion

The roughing of titanium surface by spot-blasting plus acid etching treatments contribute to higher interaction with salivary proteins, such as MG2 and lactoferrin.

Clinical significance

Titanium surface roughing increases the interactions of the substratum with salivary proteins, which can influence the integration of dental implants and their components to the oral environment. However, those treatments should be used carefully intraorally, avoiding increase biofilm formation.

How to cite this article

Cavalcanti YW, Soares RV, Assis MAL, Zenóbio EG, da Silva Girundi FM. Titanium Surface Roughing Treatments contribute to Higher Interaction with Salivary Proteins MG2 and Lactoferrin. J Contemp Dent Pract 2015;16(2):141-146.


PDF Share
  1. Physicochemical principles of tissue material interactions. Biomol Engin 2002;19:43-50.
  2. NMR analysis of human salivary mucin (MUC7) derived O-linked model glycopeptides: comparison of structural features and carbohydrate-peptide interactions. J Pept Res 1999;54:290-310.
  3. The recombinant N-terminal region of human salivary mucin MG2 (MUC7) contains a binding domain for oral Streptococci and exhibits candidacidal activity. Biochem J 2000;345:557-564.
  4. Isolation of human salivary mucin MG2 by a novel method and characterization of its interactions with oral bacteria. Arch Biochem Biophys 1999;364:286-293.
  5. Interaction of the salivary low-molecularweight mucin (MG2) with Actinobacillus actinomycetemcomitans. Antonie Van Leeuwenhoek 1996;70:79-87.
  6. MG2 and lactoferrin form a heterotypic complex in salivary secretions. J Dental Res 2003;82:1-5.
  7. Salivary micelles: identification of complexes containing MG2, sIgA, lactoferrin, amylase, glycosylated proline-rich protein and lysozyme. Arch Oral Biol 2004;49:337-343.
  8. Huma lactoferrin: a novel therapeutic with broad spectrum potential. J Pharm Pharmacol 2001;53:1303-1310.
  9. Microbiota and crevicular fluid collagenase activity in the osseointegrated dental implant sulcus: a comparison of sites in edentulous and partially edentulous patients. J Periodontal Res 1989;24:96-105.
  10. Experimental salivary pellicle formed on titanium surfaces mediate adhesion of Streptococci. Int J Oral Maxilofac Implants 1996;11:443-449.
  11. Adsorption of salivary and serum proteins, and bacterial adherence on titanium and zirconia ceramic surfaces. Clin Oral Implants Res 2008;19:780-785.
  12. Effects of saliva or serum coating on adherence of Streptococcus oralis strains to titanium. Microbiology 2012;158:390-397.
  13. Effects of titanium surface topography on bone integration: a systematic review. Clin Oral Implants Res 2009;20:172-184.
  14. On implant surfaces: a review of current knowledge and opinions. Int J Oral Maxillofac Implants 2010;25:63-74.
  15. In vivo monitoring of the bone healing process around different titanium alloy implant surfaces placed into fresh extraction sockets. J Dent 2012;40:338-346.
  16. The influence of surface treatment on the implant roughness pattern. J Appl Oral Sci 2012;20:550-555.
  17. Enhancing surface free energy and hydrophilicity through chemical modification of micro-structured titanium implant surfaces. J Biomed Mater Res A 2006;76:323-334.
  18. Surface properties of titanium and zirconia dental implant materials and their effect on bacterial adhesion. J Dent 2012;40:146-153.
  19. Influence of surface modifications to titanium on oral bacterial adhesion in vitro. J Biomed Mater Res 2000;52:388-394.
  20. The influence of surface roughness and surface-free energy on supra- and subgingival plaque formation in man: a review of the literature. J Clin Periodontol 1995;22:1-14.
  21. The influence of titanium abutment surface roughness on plaque accumulation and gingivitis: short-term observations. Int J Oral Maxillofac Implants 1996;11:169-178.
  22. Quantitative determination of protein in saliva: a comparison of analytical methods. Scand J Dent Res 1971;79:60-64.
  23. Human submandibular-sublingual saliva promotes adhesion of Candida albicans to polymethylmethacrylate. Infect Immun 1993;61:2644-2652.
  24. Biofilm formation of Candida albicans on implant overdenture materials and its removal. J Dent 2012;40:686-692.
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.