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VOLUME 16 , ISSUE 5 ( May, 2015 ) > List of Articles

RESEARCH ARTICLE

Antibacterial Potential of 2.5% Sodium Hypochlorite in Distinct Irrigation Protocols on Enterococcus faecalis Biofilm

Cyntia Rodrigues de Araújo Estrela, Ana Helena Gonçalves de Alencar, Carlos Estrela, Denise Ramos Silveira Alves, Rodrigo Sanches Cunha, Carlos Eduardo da Silveira Bueno, Tatiane Oliveira dos Santos

Citation Information : de Araújo Estrela CR, de Alencar AH, Estrela C, Alves DR, Cunha RS, da Silveira Bueno CE, Santos TO. Antibacterial Potential of 2.5% Sodium Hypochlorite in Distinct Irrigation Protocols on Enterococcus faecalis Biofilm. J Contemp Dent Pract 2015; 16 (5):340-346.

DOI: 10.5005/jp-journals-10024-1687

Published Online: 01-05-2015

Copyright Statement:  Copyright © 2015; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Objective

The aim of this study was to evaluate the effect of irrigation methods on antibacterial potential of 2.5% NaOCl on Enterococcus faecalis biofilm.

Materials and methods

Enterococcus faecalis biofilms were prepared during 60 days on 48 human root canals and randomized into control and experimental groups using positive and negative pressure irrigation. Bacterial growth was analyzed using turbidity of culture medium followed by UV spectrophotometry, and scanning electron microscopy (SEM) analyses were performed. Mean and standard deviations were used for evaluate the mean optical densities associated to the number of bacteria present culture, and Scheirer-Ray-Hare (an extension of the Kruskal-Wallis test) and Tamhane test to analyze the SEM images in the groups and thirds. Significance was set at 5%.

Results

Enterococcus faecalis was still present after root canal cleaning regardless of irrigation methods or bacterial identification methods.

Conclusion

Positive and negative pressure irrigation protocols using 2.5% NaOCl show a similar capacity to reduce E. faecalis in infected root canals.

How to cite this article

Alves DRS, Cunha RS, da Silveira Bueno CE, de Alencar AHG, de Araújo Estrela CR, dos Santos TO, Estrela C. Antibacterial Potential of 2.5% Sodium Hypochlorite in Distinct Irrigation Protocols on Enterococcus faecalis Biofilm. J Contemp Dent Pract 2015;16(5):340-346.


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