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VOLUME 16 , ISSUE 6 ( June, 2015 ) > List of Articles


Determination of Root Canal Cleanliness by Different Irrigation Methods and Morphometric Analysis of Apical Third

Carlos Estrela, Fábio Heredia Seixas, Mike Reis Bueno, Manoel Damião Sousa-Neto, Jesus Djalma Pécora

Citation Information : Estrela C, Seixas FH, Bueno MR, Sousa-Neto MD, Pécora JD. Determination of Root Canal Cleanliness by Different Irrigation Methods and Morphometric Analysis of Apical Third. J Contemp Dent Pract 2015; 16 (6):442-450.

DOI: 10.5005/jp-journals-10024-1704

Published Online: 01-11-2015

Copyright Statement:  Copyright © 2015; The Author(s).



The aim of this study was to determine the root canal area before and after the instrumentation 1 mm short of the apical foramen by clinical and cone beam computed tomography (CBCT) methods, and to evaluate the cleanliness of the apical region in mesiodistal flattened teeth by using optical microscopy.

Materials and methods

Forty-two human single-canal mandibular incisors were instrumented using the Free Tip Preparation technique up to three, four or five instruments from the initial. Cone beam computed tomography scans were acquired of the samples before and after root canal preparation (RCP). Irrigation was performed by conventional or hydrodynamic means, using 2.5% sodium hypochlorite. The samples were prepared for observation under an optical microscope. Images were digitally obtained, analyzed and the results were submitted to statistical analysis (two-way ANOVA complemented by Bonferroni's post-test).


There was no significant difference between the studied anatomical areas with both CBCT and clinical methods. There were no differences between irrigation methods. It was verified differences between instrumentation techniques. Instrumentation with four instruments from the initial instrument determined a significant increase in the contact area when compared to preparation with three instruments, but RCP with 5 instruments did not result in a better cleanliness.


The analysis with CBCT was not capable to determine the precise shape of surgical apical area comparing to the clinical method.

Clinical significance

Both the conventional and hydrodynamic irrigation techniques were not able to promote root canals debris-free. The instruments action in root canal walls was proportional to the number of instruments used from the initial apical instrument.

How to cite this article

Seixas FH, Estrela C, Bueno MR, Sousa-Neto MD, Pécora JD. Determination of Root Canal Cleanliness by Different Irrigation Methods and Morphometric Analysis of Apical Third. J Contemp Dent Pract 2015;16(6):442-450.

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