The present study was scheduled to evaluate microvascularity by CD34 expression in esophagus and oral squamous cell carcinoma.
Materials and methods
This study was scheduled using 40 paraffin blocked samples including 20 of oral SCC and 20 of esophagus ones and Immunohistochemical staining was conducted using CD34 monoclonal antibody. Exact fisher test was used to evaluate frequency of expression between two studied groups.
Results
There was significant correlation between age and tumor size with CD34 expression in oral SCC samples (p < 0.05) and no significant correlation between sex and tumor differentiation level (grading) (p > 0.05). Also, there was no significant correlation between age, sex, tumor size and tumor differentiation level (grading) with CD34 expression in esophagus SCC samples (p > 0.05). There was no significant difference of CD34 expression frequency in oral and esophagus SCC (p = 0/583). Finally, CD34 expression was reported ‘high’ for major cases of esophagus and oral SCCs.
Conclusion
It seems, other angiogenetic or nonangiogenetic factors except CD34 may play more important role and explain the different clinical behavior of SCC at recent different locations.
Clinical significance
Other factors would be considered along with CD34 expression to interpret different clinical behavior of SCC at recent different locations.
How to cite this article
Shahsavari F, Farhadi S, Sadri D, Sedehi M, Evaluation of Microvascularity by CD34 Expression in Esophagus and Oral Squamous Cell Carcinoma. J Contemp Dent Pract 2015;16(6):458-462.
Reduced expression of cyclin-depended kinase inhibitor P27 Kip1 is an indirector of malignant behavior in oral squamous cell carcinoma. Cancer 1998 Dec 15;83(12):2447-2455.
Down regulation of Cdk inhibitor P27 in oral squamous cell carcinoma. Oral Oncology 2005 Feb;41(2):105-116.
Bethesda, MD, National Cancer Institute, 2005 [updated 2006; cited March and May 2006]. Available at: http://seer.cancer.gov/csr/1975_2003/.
Focus on head and neck cancer. Cancer Cell 2004;5:311.
Significance of immunohistochemical expression of P27 and involucrine as the maker of cellular differention of squamous cell carcinoma of the esophagus. Oncology 2006;71(5-6):402-410.
Chemoprevention of esophageal squamous cell carcinoma. Toxicol Appl Pharmacol 2007;224:337-349.
Oral. Bouquot J and maxillofacial pathology. Philadelphia: Saunders. 3rd ed; 2009. p. 409-421.
Esophagealcancer. N Engl J Med 2003;349:2241-2252.
Evaluation of microvascular density by CD34 in squamous cell carcinoma of the tongue and its relationship with cervical lymph node metastasis. J Dent Med Winter 2009;21[4(57)]:233-241.
III. Vascular reactions of mice to fibroblasts treated in vitro with methylcholanthrene. JNCI 1950;11:555.
Immunolocalisation of tissue factor in esophageal cancer is correlated with intratumoral angiogenesis and prognosis of the patient. Acta Histochemical 2010 May;112(3):233-239.
The biology of vascular endothelial growth factor. Endocr Rev 1997;18:4-25.
Vascular endo the lial growth factor: its prognostic, predictive, and the rapeutic implications. Lancet Oncol 2001;2:667-673.
Oral Oncol 1997;33:369.
assessment of vascularity in histological sections: Effects of methodology and value as an index of angiogenesis in breast tumours. Histochem J 1998;30:849.
CD34 and caspase-3 biomarkers expression in esophageal epidermoid carcinoma. Rev col Bras cir, 2009 Feb;36(1):35-41.
Expression of non-mast cell histidine decarboxylase in tumor-associated microvessels in human esophageal squamous cell carcinomas APMIS 2008 Dec;116(12):1034-1042.
Angiogenesis and CD34 expression as a predictor of recurrence in oral squamous cell carcinoma. J Oral Maxillofac Surg 2009 Sep;67(9):1800-1805.
Comparative immunohistochemical analysis of angiogenesis and mast cell density in oral normal mucosa and squamous cell carcinoma Dent Res J (Isfahan) 2012 Jan;9(1):8-12. doi: 10.4103/1735-3327.92920.
Expression of mmp-9, TIMP-1, CD34 and factor 8 as prognostic marker for squamous cell carcinoma of the tongue. Journal of APMIS 2004;116:1034-1042.