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VOLUME 24 , ISSUE 9 ( September, 2023 ) > List of Articles


Tracing of Microbes in Prepared Cavity Following Different Minimally Invasive Caries Removal Protocols

Md Sofiqul Islam, Zainab Riadh, Dina Thaer Abdulsalam Al-Ajeeli, Ashfaque Hossain, Vivek Padmanabhan

Keywords : Caries detection dye, Chemo-mechanical caries removal, Minimally invasive caries removal, Residual microbes, Visual tactile

Citation Information : Islam MS, Riadh Z, Al-Ajeeli DT, Hossain A, Padmanabhan V. Tracing of Microbes in Prepared Cavity Following Different Minimally Invasive Caries Removal Protocols. J Contemp Dent Pract 2023; 24 (9):674-678.

DOI: 10.5005/jp-journals-10024-3569

License: CC BY-NC 4.0

Published Online: 13-10-2023

Copyright Statement:  Copyright © 2023; The Author(s).


Aims: The conventional caries removal technique has been replaced with minimally invasive (MI) techniques to preserve healthy natural teeth and to provide durable dental restorations. Each of these MI caries removal protocols is reported to be favorable in dealing with different caries conditions. The current study aimed to trace the residual bacteria that may remain in a prepared cavity following a visual-tactile (VT), caries detection dye (CDD), and chemo-mechanical caries removal (CMCR) protocol. Materials and methods: A total of 29 extracted human molar teeth with visible caries lesions were randomly divided into three groups. The cavity preparation and caries removal of each group was accomplished following one of the MI caries removal protocols. Swab samples (one from each specimen) were taken and inoculated onto a blood agar plate and incubated for 48 hours. The growth of the bacterial colony was observed under a microscope and the specific genome of the bacteria was identified by polymerase chain reaction (PCR) test. Results: The maximum number of traceable bacteria was observed following the chemo-mechanical caries removal group followed by the caries detection dye group and the least in the visual-tactile group. The PCR test revealed the presence of Streptococcus mutans in all the observed colonies; however, Streptococcus sobrinus was absent completely. The Chi-square test reveals a statistically insignificant (p = 0.646) difference among the tested groups. Conclusion: All of the MI caries removal protocols used in this study showed a trace of microbes in certain teeth. The cavity prepared following a visual tactile protocol showed the least amount of traceable bacteria in the prepared cavity. Clinical significance: Cavity that is prepared following individual MI protocol has a risk of leaving microbes in it.

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