The Journal of Contemporary Dental Practice

Register      Login

SEARCH WITHIN CONTENT

FIND ARTICLE

Volume / Issue

Online First

Archive
Volume  25 (2024)
Volume  24 (2023)
Volume  23 (2022)
Volume  22 (2021)
Volume  21 (2020)
Volume  20 (2019)
Volume  19 (2018)
Volume  18 (2017)
Volume  17 (2016)
Volume  16 (2015)
Volume  15 (2014)
Volume  14 (2013)
Volume  13 (2012)
Volume  12 (2011)
Volume  11 (2010)
Volume  10 (2009)
Volume  9 (2008)
Volume  8 (2007)
Volume  7 (2006)
Volume  6 (2005)
Volume  5 (2004)
Volume  4 (2003)
Volume  3 (2002)
Volume  2 (2001)
Volume  1 (1999)
Related articles

VOLUME 24 , ISSUE 9 ( September, 2023 ) > List of Articles

ORIGINAL RESEARCH

Tracing of Microbes in Prepared Cavity Following Different Minimally Invasive Caries Removal Protocols

Md Sofiqul Islam, Zainab Riadh, Dina Thaer Abdulsalam Al-Ajeeli, Ashfaque Hossain, Vivek Padmanabhan

Keywords : Caries detection dye, Chemo-mechanical caries removal, Minimally invasive caries removal, Residual microbes, Visual tactile

Citation Information : Islam MS, Riadh Z, Al-Ajeeli DT, Hossain A, Padmanabhan V. Tracing of Microbes in Prepared Cavity Following Different Minimally Invasive Caries Removal Protocols. J Contemp Dent Pract 2023; 24 (9):674-678.

DOI: 10.5005/jp-journals-10024-3569

License: CC BY-NC 4.0

Published Online: 13-10-2023

Copyright Statement:  Copyright © 2023; The Author(s).


Abstract

Aims: The conventional caries removal technique has been replaced with minimally invasive (MI) techniques to preserve healthy natural teeth and to provide durable dental restorations. Each of these MI caries removal protocols is reported to be favorable in dealing with different caries conditions. The current study aimed to trace the residual bacteria that may remain in a prepared cavity following a visual-tactile (VT), caries detection dye (CDD), and chemo-mechanical caries removal (CMCR) protocol. Materials and methods: A total of 29 extracted human molar teeth with visible caries lesions were randomly divided into three groups. The cavity preparation and caries removal of each group was accomplished following one of the MI caries removal protocols. Swab samples (one from each specimen) were taken and inoculated onto a blood agar plate and incubated for 48 hours. The growth of the bacterial colony was observed under a microscope and the specific genome of the bacteria was identified by polymerase chain reaction (PCR) test. Results: The maximum number of traceable bacteria was observed following the chemo-mechanical caries removal group followed by the caries detection dye group and the least in the visual-tactile group. The PCR test revealed the presence of Streptococcus mutans in all the observed colonies; however, Streptococcus sobrinus was absent completely. The Chi-square test reveals a statistically insignificant (p = 0.646) difference among the tested groups. Conclusion: All of the MI caries removal protocols used in this study showed a trace of microbes in certain teeth. The cavity prepared following a visual tactile protocol showed the least amount of traceable bacteria in the prepared cavity. Clinical significance: Cavity that is prepared following individual MI protocol has a risk of leaving microbes in it.

HTML PDF Share
  1. Pitts NB, Twetman S, Fisher J, et al. Understanding dental caries as a non-communicable disease. Br Dent J 2021;231(12):749–753. DOI: 10.1038/s41415-021-3775-4.
  2. Hon L, Mohamed A, Lynch E. Reliability of colour and hardness clinical examinations in detecting dentine caries severity: A systematic review and meta-analysis. Sci Rep 2019;9(1):6533. DOI: 10.1038/s41598-019-41270-6.
  3. Ritter AV, Boushell LW, Walter R. Sturdevant's art and science of operative dentistry, 7th edition. 2019. 120p. ELSEVIER, USA.
  4. Innes NPT, Frencken JE, Bjørndal L, et al. Managing carious lesions: Consensus recommendations on terminology. Adv Dent Res 2016;28(2):49–57. DOI: 10.1177/0022034516639276.
  5. Ricketts D, Innes N, Schwendicke F. Selective removal of carious tissue. Monogr Oral Sci 2018;27:82–91. DOI: 10.1159/000487838.
  6. Neves ADA, Coutinho E, De Munck J, et al. Caries-removal effectiveness and minimal-invasiveness potential of caries-excavation techniques: A micro-CT investigation. J Dent 2011;39(2):154–162. DOI: 10.1016/j.jdent.2010.11.006.
  7. Schwendicke F, Schweigel H, Petrou MA, et al. Selective or stepwise removal of deep caries in deciduous molars: Study protocol for a randomized controlled trial. Trials 2015;16(1):11. DOI: 10.1186/s13063-014-0525-9.
  8. Banerjee A, Kidd EAM, Watson TF. In vitro evaluation of five alternative methods of carious dentine excavation. Caries Res 2000;34(2): 144–150. DOI: 10.1159/000016582.
  9. Innes NPT, Schwendicke F. Restorative thresholds for carious lesions: Systematic review and meta-analysis. J Dent Res 2017;96(5):501–508. DOI: 10.1177/0022034517693605.
  10. Srilatha A, Doshi D, Kulkarni S, et al. Conventional diagnostic aids in dental caries. J Glob Oral Heal 2019;2(1):53–58. DOI: 10.25259/JGOH_42_2019.
  11. Neuhaus KW, Ellwood R, Lussi A, et al. Traditional lesion detection aids. Monogr Oral Sci 2009;21:42–51. DOI: 10.1159/000224211.
  12. Amaechi BT. Emerging technologies for diagnosis of dental caries: The road so far. J Appl Phys 2009;105(10):102047. DOI: 10.1063/1.3116632.
  13. Lennon ÁM, Attin T, Buchalla W. Quantity of remaining bacteria and cavity size after excavation with FACE, caries detector dye and conventional excavation in vitro. Oper Dent 2007;32(3):236–241. DOI: 10.2341/06-64.
  14. Kumar KVKS, Prasad MG, Sandeep VV, et al. Chemomechanical caries removal method versus mechanical caries removal methods in clinical and community-based setting: A comparative in vivo study. Eur J Dent 2016;10(3):386–391. DOI: 10.4103/1305-7456.184151.
  15. Ritter A V, Boushell LW, Walter R. Fundamental of tooth preparation. In: Sturdevant's Art and Science of Operative Dentistry, 7th edition. 2019. p. 129. ELSEVIER, USA.
  16. Schwendicke F, Dörfer CE, Paris S. Incomplete caries removal: A systematic review and meta-analysis. J Dent Res 2013;92(4):306–314. DOI: 10.1177/0022034513477425.
  17. Ntovas P, Loubrinis N, Maniatakos P, et al. Evaluation of dental explorer and visual inspection for the detection of residual caries among Greek dentists. J Conserv Dent 2018;21(3):311–318. DOI: 10.4103/JCD.JCD_67_17.
  18. Peskersoy C, Turkun M, Onal B. Comparative clinical evaluation of the efficacy of a new method for caries diagnosis and excavation. J Conserv Dent 2015;18(5):364–368.
  19. Ganter P, Al-Ahmad A, Wrbas KT, et al. The use of computer-assisted FACE for minimal-invasive caries excavation. Clin Oral Investig 2014;18(3):745–751. DOI: 10.1007/s00784-013-1022-3.
  20. Gurbuz T, Yilmaz Y, Sengul F. Performance of laser fluorescence for residual caries detection in primary teeth. Eur J Dent 2008;02(03): 176–184.
  21. Iwami Y, Shimizu A, Narimatsu M, et al. The relationship between the color of carious dentin stained with a caries detector dye and bacterial infection. Oper Dent 2005;30(1):83–89.
  22. Banerjee A, Kidd EAM, Watson TF. In vitro validation of carious dentin removed using different excavation criteria. Am J Dent 2003;16(4):228–230.
  23. Yip HK, Stevenson AG, Beeley JA. The specificity of caries detector dyes in cavity preparation. Br Dent J 1994;176(11):417–421. DOI: 10.1038/sj.bdj.4808470.
  24. Javaheri M, Maleki-Kambakhsh S, Etemad-Moghadam S. Efficacy of two caries detector dyes in the diagnosis of dental caries. J Dent (Tehran) [Internet] 2010;7(2):71–76. Available from: http://www.ncbi.nlm.nih.gov/pubmed/21998778%0Ahttp://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC3184748.
  25. Ganesh M, Parikh D. Chemomechanical caries removal (CMCR) agents: Review and clinical application in primary teeth. J Dent Oral Hyg 2011;3(March):34–45.
  26. Nagaveni N, Radhika N, Satisha T, et al. Efficacy of new chemomechanical caries removal agent compared with conventional method in primary teeth: An in vivo study. Int J Oral Heal Sci 2016;6(2):52. DOI: 10.4103/2231-6027.199986.
  27. Sadasiva K, Kumar K, Rayar S, et al. Evaluation of the efficacy of visual, tactile method, caries detector dye, and laser fluorescence in removal of dental caries and confirmation by culture and polymerase chain reaction: An in vivo study. J Pharm Bioallied Sci 2019;11(6):S146–S150. DOI: 10.4103/JPBS.JPBS_279_18.
  28. Zacharia MA, Munshi AK. Microbiological assessment of dentin stained with a caries detector dye. J Clin Pediatr Dent 1995;19(2): 111–115.
  29. Kidd EA, Joyston-Bechal S, Smith MM, et al. The use of a caries detector dye in cavity preparation. Br Dent J 1989;167(4):132–134. DOI: 10.1038/sj.bdj.4806939.
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.