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VOLUME 16 , ISSUE 8 ( August, 2015 ) > List of Articles


Is It Possible to induce Artificial Caries-affected Dentin using the Same Protocol to Primary and Permanent Teeth?

Tathiane Larissa Lenzi, Fabio Zovico Maxnuck Soares, Tamara Kerber Tedesco, Rachel de Oliveira Rocha

Citation Information : Lenzi TL, Soares FZ, Tedesco TK, Rocha RD. Is It Possible to induce Artificial Caries-affected Dentin using the Same Protocol to Primary and Permanent Teeth?. J Contemp Dent Pract 2015; 16 (8):638-642.

DOI: 10.5005/jp-journals-10024-1734

Published Online: 01-01-2016

Copyright Statement:  Copyright © 2015; The Author(s).



This in vitro study compared the mineral loss of natural and artificially-created caries-affected dentin in primary and permanent teeth using the same protocol to induce caries lesions.

Materials and methods

Twenty molars presenting natural occlusal dentin caries lesions (10 primary–PriC and 10 permanent– PermC; control group), and 20 sound molars (10 primary – PripH and 10 permanent–PermpH; experimental group), were selected. Occlusal cavities were prepared in teeth of the experimental group that were submitted to pH-cycling for 14 days to simulate caries-affected dentin. All specimens were longitudinally sectioned and prepared in order to obtain Knoop microhardness values from 15 to 250 ìm depth, starting in bottom of center of natural lesions or cavities. The microhardness (KHN) data were submitted to three-way repeated measures analysis of variance (ANOVA) and Tukey's tests (α = 0.05).


Considering all depths, there was no statistically significant differences (p > 0.05) between the mineral loss of the control (PriC = 30.9 ± 6.4 and PermC = 40.8 ± 8.6) and experimental (PripH = 27.3 ± 11.1 and PermpH = 35.5 ± 14.0) groups, neither between primary and permanent teeth.


The mineral loss of the artificially-created cariesaffected dentin is similar to that from naturally developed dentin caries lesions.

Clinical significance

The pH-cycling model may be a suitable method to simulate caries-affected dentin in both permanent and primary teeth.

How to cite this article

Lenzi TL, Soares FZM, Tedesco TK, de Oliveira Rocha R. Is It Possible to induce Artificial Caries-affected Dentin using the Same Protocol to Primary and Permanent Teeth? J Contemp Dent Pract 2015;16(8):638-642.

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