Fibrinogen Demonstration in Oral Lichen Planus: An Immunofluorescence Study on Archival Tissues
Alka Kale, Pallavi D Shirol, Veena Naik
Citation Information :
Kale A, Shirol PD, Naik V. Fibrinogen Demonstration in Oral Lichen Planus: An Immunofluorescence Study on Archival Tissues. J Contemp Dent Pract 2015; 16 (10):824-828.
Lichen planus is a premalignant condition with minimal diagnostic aids. This study is an attempt to use paraffin embedded sections of lichen planus with immunofluorescein stain and to evaluate the immunofluorescent sections to establish pattern of fibrinogen deposition.
Materials and methods
Thirty-five paraffin embedded sections of old and new cases of oral lichen planus (study group) and five normal oral mucosa (control group) were chosen. Two sections of each (H & E) case were taken, one was stained with hematoxylin and eosin and another with fluorescein isothiocynate conjugate (FITC) polyclonal rabbit antibody against fibrinogen. Fluorescent findings were examined with a fluorescent microscope.
Results
A high statistical significant correlation was found in respect to fluorescence positivity, intensity of fluorescence and distribution of fluorescence each with p < 0.0001 and fluorescence at blood vessel walls (p = 0.0003).
Conclusion
This study suggested that paraffin embedded sections can be successfully used in direct immunofluorescence staining in routine set up where only formalin fixed tissues are received.
Clinical significance
Paraffin embedded sections can be successfully used in direct immunofluorescence staining when only formalin fixed tissues are received.
How to cite this article
Shirol PD, Naik V, Kale A. Fibrinogen Demonstration in Oral Lichen Planus: An Immunofluorescence Study on Archival Tissues. J Contemp Dent Pract 2015;16(10): 824-828.
Direct immunofluorescence of oral mucosal biopsies: a comparison of fresh-frozen tissue and formalin-fixed, paraffin-embedded tissue. J Oral Pathol Med 1992 Sep;21(8):358-363.
Assessment of the value of immunofluorescence microscopy in the diagnosis of oral mucosal lichen planus. J Oral Pathol Med 1990 Aug;19(7):295-297.
Immunofluorescence in oral lichen planus and oral lichenoid reaction: a review. Ind J Dent Res 2001 Jan-Mar;12(1):29-34.
III, The sensitivity and specificity of direct immunofluorescence testing in disorders of mucous membranes. J Am Acad Dermatol 1994 Jan;30(1):65-75.
Immunopathogenesis of oral lichen planus. J Oral Pathol Med 1990;19:389-396.
Robbins basic pathology. 7th ed; Elsevier Publishers, 2003. p. 119-120.
The diagnostic significance of immunoglobulin and fibrin deposition in lichen planus. Br J Dermatol 1975 Jul;93(1):17-24.
Direct immunofluorescence in oral mucosal disease: a diagnostic analysis of 130 cases. Oral Surg Oral Med Oral Pathol 1981 Jan;51(1):38-47.
The reliability of immunofluorescence and histopathology in the diagnosis of discoid lupus erythematosus and lichen planus. Br J Dermatol 1987 Feb;116(2):189-198.
Direct immunofluorescence in oral lichen planus and oral lichenoid reactions. Quintessence Int 2002 Mar;33(3):234-239.
http://www.serotech.com
Antigen retrieval techniques: current perspectives. J Histochem Cytochem 2001 Aug;49(8):931-937.
A universal diagnostic criteria for oral lichen planus: an exigency! Int J Contemp Dent Med Rev Vol. 2014. 2014.