The Journal of Contemporary Dental Practice

Register      Login

SEARCH WITHIN CONTENT

FIND ARTICLE

Volume / Issue

Online First

Archive
Related articles

VOLUME 16 , ISSUE 11 ( November, 2015 ) > List of Articles

RESEARCH ARTICLE

Influence of Concentration and Agitation of Sodium Hypochlorite and Peracetic Acid Solutions on Tissue Dissolution

Mario Tanomaru-Filho, Juliane Maria Guerreiro-Tanomaru, Bruna Ramos Franco Silveira, Roberta Bosso Martelo

Citation Information : Tanomaru-Filho M, Guerreiro-Tanomaru JM, Silveira BR, Martelo RB. Influence of Concentration and Agitation of Sodium Hypochlorite and Peracetic Acid Solutions on Tissue Dissolution. J Contemp Dent Pract 2015; 16 (11):876-879.

DOI: 10.5005/jp-journals-10024-1775

Published Online: 01-04-2016

Copyright Statement:  Copyright © 2015; The Author(s).


Abstract

Aim and objective

To evaluated the tissue dissolution of sodium hypochlorite (NaOCl) and peracetic acid (PA) solutions at different concentrations, with or without ultrasonic agitation.

Materials and methods

The following solutions were analyzed: 2.5% NaOCl, 0.5, 1 and 2% PA, 1% PA associated with 6.5% hydrogen peroxide (HP) and saline. Fragments of bovine pulp tissue with 25 ± 2g mg were immersed into test tubes containing 4 mL of the solutions for 10 minutes. In the groups with agitation, pulp tissues were submitted to 2 cycles of 1 minute of ultrasonic agitation. The specimens were weighed after the removal from the solutions. The percentage of mass loss was calculated according to the difference of mass before and after exposure to solutions. Data were submitted to ANOVA and Tukey tests (p . 0.05).

Results

A total of 2.5% NaOCl with or without agitation showed the higher tissue dissolution (between 64.5 and 67% of mass reduction) (p < 0.005). By comparing the PA solutions, the concentrations of 1 and 2% with or without agitation and the concentration of 0.5% with agitation showed similar dissolution activity (between 35.4 and 44% of mass reduction). The use of the ultrasonic agitation promoted an increase of the dissolution ability only for 0.5% PA.

Conclusion

Peracetic acid solution has pulp tissue dissolution. However, this ability is lower than 2.5% NaOCl solution.

Clinical significance

The sodium hypochlorite solution shows higher ability to dissolve tissue than PA.

How to cite this article

Tanomaru-Filho M, Silveira BRF, Martelo RB, Guerreiro-Tanomaru JM. Influence of Concentration and Agitation of Sodium Hypochlorite and Peracetic Acid Solutions on Tissue Dissolution. J Contemp Dent Pract 2015;16(11):876-879.


PDF Share
  1. Effects of calcium hydroxide and sodium hypochlorite on the dissolution of necrotic porcine muscle tissue. J Endod 1988 Mar;14(3):125-127.
  2. The peptidolytic capacity of the spirochete system. Med Microbiol Immunol 1996 May;185(1):1-10.
  3. Dissolution of pulp tissue by aqueous solution of chlorhexidine digluconate and chlorhexidine digluconate gel. Int Endod J 2004 Jan;37(1):38-41.
  4. In vivo evaluation of microbial reduction after chemomechanical preparation of human root canals containing necrotic pulp tissue. Int Endod J 2006 Jun;39(6):484-492.
  5. Comparative study of the antimicrobial effect of three irrigant solutions (chlorhexidine, sodium hypochlorite and chlorhexidinated MUMS). J Contemp Dent Pract 2012 Jul;13(4):436-439.
  6. Soft tissue dissolution capacity of currently used and potential endodontic irrigants. J Endod 2004 Nov;30(11):785-787.
  7. An in vitro evaluation of the cytotoxicity of various endodontic irrigants on human gingival .broblasts. J Endod 2005 Aug;31(8):613-615.
  8. The effects of temperature on sodium hypochlorite short-term stability, pulp dissolution capacity, and antimicrobial efficacy. J Endod 2005 Sep;31(9):669-671.
  9. Use of a bottle warmer to increase 4% sodium hypochlorite tissue dissolution ability on bovine pulp. Aust Endod J 2008 Apr;34(1):39-42.
  10. Effect of lowering the pH of sodium hypochlorite on dissolving tissue in vitro. J Endod 2008 Apr;34(4):449-452.
  11. Stabilizing sodium hypochlorite at high pH: effects on soft tissue and dentin. J Endod 2008 May;37(5):693-696.
  12. Root canal irrigants. J Endod 2006 May;32(5):389-398.
  13. Passive ultrasonic irrigation of the root canal: a review of the literature. Int Endod J 2007 Jun;40(6):415-426.
  14. Necrotic pulp tissue dissolution by passive ultrasonic irrigation in simulated accessory canals: impact of canal location and angulation. Int Endod J 2009 Jan;42(1):59-65.
  15. Factors promoting the tissue dissolving capability of sodium hypochlorite. Int Endod J 1982 Oct;15(4):187-196.
  16. Antibacterial effectiveness of peracetic acid and conventional endodontic irrigants. Braz Dent J 2011;22(4):285-287.
  17. Antibacterial efficacy of endodontic irrigating solutions and their combinations in root canals contaminated with Enterococcus faecalis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2011 Sep;112(13):396-400.
  18. Antiseptics and disinfectants: activity, action, and resistance. Clin Microbiol Rev 1999 Jan;12(1):147-179.
  19. Strong effect on dentin after the use of high concentrations of citric acid: an assessment with co-site optical microscopy and ESEM. Dent Mater 2008 Dec;24(12):1608-1615.
  20. Effects of ethylenediaminetetraacetic, etidronic and peracetic acid irrigation on human root dentine and the smear layer. Int Endod J 2009 Apr;42(4):335-343.
  21. Smear layer dissolution by peracetic acid of low concentration. Int Endod J 2011 Jun;44(6):485-490.
  22. Comparison of organic tissue dissolution capacities of sodium hypochlorite and chlorine dioxide. J Endod 2010 Feb;36(2):272-274.
  23. Dissolution of bovine pulp tissue by endodontic solutions. Scand J Dent Res 1980 Oct;88(5):406-411.
  24. Quantitative analysis of the solubilizing action of MTAD, sodium hypochlorite, and EDTA on bovine pulp and dentin. J Endod 2003 May;29(5):334-337.
  25. Physicochemical and pulp tissue dissolution properties of some household bleach brands compared with a dental sodium hypochlorite solution. J Endod 2012 Mar;38(3):372-375.
  26. Microbiological evaluation of one- and two-visit endodontic treatment of teeth with apical periodontitis: a randomized, clinical trial. J Endod 2004 Aug;30(8):572-576.
  27. Effectiveness of root canal debris removal using passive ultrasound irrigation with chlorhexidine digluconate or sodium hypochlorite individually or in combination as irrigants. J Contemp Dent Pract 2008 Jul;9(5):68-75.
  28. Shelf life, dissolving action, and antibacterial activity of a neutralized 2.5% sodium hypochlorite solution. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2009 Aug;108(2):66-73.
  29. In vivo and in vitro evaluation of the efficacy of a peracetic acid-based disinfectant for decontamination of acrylic resins. Braz Dent J 2006;17(2):117-121.
  30. Cytotoxicity of liquid disinfectants. Surg Infect 2000;1(1):3-14.
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.