The Journal of Contemporary Dental Practice

Register      Login



Volume / Issue

Online First

Related articles

VOLUME 17 , ISSUE 6 ( June, 2016 ) > List of Articles


In vitro Analysis of Cytotoxicity of Temporary Resilient Relining Materials

Isleine P Caldas, Miriam Z Scelza, Marco A Gallito, Gutemberg Alves, Licínio Silva

Citation Information : Caldas IP, Scelza MZ, Gallito MA, Alves G, Silva L. In vitro Analysis of Cytotoxicity of Temporary Resilient Relining Materials. J Contemp Dent Pract 2016; 17 (6):457-462.

DOI: 10.5005/jp-journals-10024-1872

Published Online: 01-10-2016

Copyright Statement:  Copyright © 2016; The Author(s).



The aim of this study is to evaluate the in vitro response of human gingival fibroblasts in primary cultures to two materials for temporary relining of dentures: Temporary Soft (TDV, Brazil) and Trusoft (Bosworth, USA) for 24 hours, 7 and 30 days by using a multi-parametric analysis.

Materials and methods

Each material sample (TDV, TS, Polystyrene, Latex) was prepared and incubated in a culture medium for 1, 7, and 30 days at 37°C. Human gingival fibroblasts were exposed to the extracts and cell viability was evaluated by a multi-parametric assay, which allowed sequential analysis of mitochondrial activity (XTT), membrane integrity [neutral red (NR)], and cell density [crystal violet dye exclusion (CVDE)] in the same cells. Analysis of variance (ANOVA) was used to test the interactions of the three sources of variation (material, test method, and time) with the proportions of viable cells for each relining material.


Both evaluated materials (TDV and TS) had low cytotoxic effects during 1, 7, and 30 days after manipulation of the material, as assessed by all three methods used. A statistical difference was found when comparing the negative control group (latex fragments) with the other groups, which showed high toxicity and low percentage of cell viability in all tests used. There was no significant difference among other materials (p > 0.05).


Low cytotoxicity levels were detected by representatives of the major groups of temporary prosthetic relining materials, as evaluated by multiple cellular viability parameters in human fibroblasts.

Clinical significance

There are various soft materials on the market for relining prostheses; however, the effects of these materials on tissues need to be clarified to avoid problems for patients.

How to cite this article

Caldas IP, Scelza MZ, Gallito MA, Alves G, Silva L. In vitro Analysis of Cytotoxicity of Temporary Resilient Relining Materials. J Contemp Dent Pract 2016;17(6):457-462.

PDF Share
  1. Randomized controlled clinical trial for verifying the effect of silicone-based resilient denture liner on the masticatory function of complete denture wearers. Int J Prosthodont 2006 Nov-Dec;19(6):593-600.
  2. Part 5: Tests for in vitro cytotoxicity; 2009. [Accessed 2014 Feb 24]. Available from:
  3. Part 12: Sample preparation and reference materials; 2002. [Accessed 2014 Feb 24]. Available from: 35331&ICS1=11&ICS2=100&ICS3=20.
  4. Sorption and solubility of 12 soft denture liners. J Prosthet Dent 1994 Oct;72(4):393-398.
  5. In vitro cytotoxicity of tissue conditioners. J Prosthet Dent 1991 Nov;66(5):656-659.
  6. Estrogenic activity of tissue conditioners in vitro. Dent Mater 2003 Jun;19(4):341-346.
  7. Allergic sensitization of the skin and oral mucosa to acrylic denture materials. J Am Med Assoc 1954 Sep;156(3):238-242.
  8. Aspiration pneumonia: dental and oral risk factors in an older veteran population. J Am Geriatr Soc 2001 May;49(5):557-563.
  9. The effect of complete dentures on alveolar mucosa. J Prosthet Dent 1963 Nov-Dec;13(6):1030-1037.
  10. Avaliação multiparamétrica da citotoxicidade in vitro de um fio ortodôntico à base de resina polimérica reforçada com fibra de vidro. Innov Implant J Biomater Esthet 2010;5:35-40.
  11. A multiparametric assay to compare the cytotoxicity of endodontic sealers with primary human osteoblasts. Int Endod J 2012 Jan;45(1):12-18.
  12. Phillips’ science of dental materials. 11th ed. Amsterdam: Saunders; 2006. p. 174,753-754.
  13. Biologic assessment of restorative dental materials: interrelationship of biologic and technologic properties. Oper Dent 1978;3:9-13.
  14. Cytotoxic effects of resin components on cultured mammalian fibroblasts. J Dent Res 1991 Nov;70(11):1450-1455.
  15. Cytotoxic effects of denture base materials on a permanent human oral epithelial cell line and on primary human oral fibroblasts in vitro. Int J Prosthodont 2001 Sep-Oct;14(5):439-443.
  16. Cytocompatibility of a tissue conditioner containing vinyl ester as a plasticizer. Dent Mater J 2007 Nov;26(6):785-791.
  17. Estudo preliminar da influência do laser de baixa intensidade (GaAlAs) na proliferação de células derivadas de polpa dental decídua humana. Innov Implant J, Biomater Esthet 2009;4:48-52.
  18. Evaluation of cytocompatibility of bioglassniobium granules with human primary osteoblasts: a multiparametric approach. Key Eng Mater 2012;494:37-42.
  19. Cytotoxicity of hard and soft denture lining materials. Dent Mater J 2012;31(6):1082-1086.
  20. Effect of water storage and heat treatment on the cytotoxicity of soft liners. Gerodontology 2012 Jun;29(2):e275-e280.
  21. Cytotoxicity of soft denture lining materials depending on their component types. Int J Prosthodont 2014 May-Jun;27(3):229-235.
  22. Comparative study of water sorption, solubility, and tensile bond strength of two soft lining materials. J Prosthet Dent 2000 Mar;83(3):356-361.
  23. Effect of aging on tear strength and cytotoxicity of soft denture lining materials; in vitro. J Adv Prosthodont 2014 Apr;6(2):115-120.
  24. Influence of monomer content on the viscoelasticity, water sorption and solubility of experimental fluorinated soft lining materials. Dent Mater J 2015;34(1):70-77.
  25. Effect of aging on bond strength of two soft lining materials to a denture base polymer. J Indian Prosthodont Soc 2014 Dec;14(Suppl 1):S155-S160.
  26. In vitro evaluation of cytotoxicity of soft lining materials on L929 cells by MTT assay. J Biomed Mater Res B Appl Biomater 2009 Jul;90(1):82-86.
  27. Cytotoxicity of denture base and hard chairside reline materials: a systematic review. J Prosthet Dent 2012 Feb;107(2):114-127.
  28. Cytotoxicity of denture base resins. Int J Prosthodont 1997 Jan-Feb;10(1):73-77.
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.