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VOLUME 17 , ISSUE 7 ( July, 2016 ) > List of Articles


An in vitro Comparison of Antimicrobial Activity of Three Endodontic Sealers with Different Composition

Iti Gupta, Gurpreet Singh, Faheim MM Elshamy, Husham E Homeida, Nezar Boreak

Citation Information : Gupta I, Singh G, Elshamy FM, Homeida HE, Boreak N. An in vitro Comparison of Antimicrobial Activity of Three Endodontic Sealers with Different Composition. J Contemp Dent Pract 2016; 17 (7):553-556.

DOI: 10.5005/jp-journals-10024-1888

Published Online: 01-07-2016

Copyright Statement:  Copyright © 2016; The Author(s).



The aim of the study was to compare the antimicrobial property of newly introduced EndoSequence BC sealer with commonly used zinc oxide-eugenol-based sealer (Zical) and epoxy resin-based sealer (MM-Seal) against Candida albicans, Lactobacillus, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa.

Materials and methods

The agar diffusion test was done to measure the antimicrobial activity of sealers. The sealers were put in the 4 mm wells prepared in the inoculated agar plates. The agar plates were incubated at 37°C for 24 hours and the zones of inhibition that appeared was measured. Chi-square test was done to evaluate intraobserver bias for all study samples. Intergroup comparison was done for all five parameters using Pearson correlation statistical analysis.


EndoSequence BC sealer showed maximum mean of diameter of zones of inhibition against all the microorganisms but with no statistically significant difference with other sealers. Zical did not show any zone of inhibition against the P. aeruginosa. MM-Seal did not show any inhibitory activity against the P. aeruginosa and C. albicans.


EndoSequence BC sealer showed antimicrobial activity against all the microorganisms and proved to be a better choice when compared with other two sealers.

Clinical significance

Antimicrobial properties of endodontic sealers are important to prevent the persistent infection of the complex root canals. EndoSequence BC sealer has been proved to be a better sealer in this aspect.

How to cite this article

Singh G, Elshamy FMM, Homeida HE, Boreak N, Gupta I. An in vitro Comparison of Antimicrobial Activity of Three Endodontic Sealers with Different Composition. J Contemp Dent Pract 2016;17(7):553-556.

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