The Journal of Contemporary Dental Practice

Register      Login

SEARCH WITHIN CONTENT

FIND ARTICLE

Volume / Issue

Online First

Archive
Related articles

VOLUME 17 , ISSUE 7 ( July, 2016 ) > List of Articles

RESEARCH ARTICLE

An in vitro Comparison of Antimicrobial Activity of Three Endodontic Sealers with Different Composition

Iti Gupta, Gurpreet Singh, Faheim MM Elshamy, Husham E Homeida, Nezar Boreak

Citation Information : Gupta I, Singh G, Elshamy FM, Homeida HE, Boreak N. An in vitro Comparison of Antimicrobial Activity of Three Endodontic Sealers with Different Composition. J Contemp Dent Pract 2016; 17 (7):553-556.

DOI: 10.5005/jp-journals-10024-1888

Published Online: 01-07-2016

Copyright Statement:  Copyright © 2016; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Aims

The aim of the study was to compare the antimicrobial property of newly introduced EndoSequence BC sealer with commonly used zinc oxide-eugenol-based sealer (Zical) and epoxy resin-based sealer (MM-Seal) against Candida albicans, Lactobacillus, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa.

Materials and methods

The agar diffusion test was done to measure the antimicrobial activity of sealers. The sealers were put in the 4 mm wells prepared in the inoculated agar plates. The agar plates were incubated at 37°C for 24 hours and the zones of inhibition that appeared was measured. Chi-square test was done to evaluate intraobserver bias for all study samples. Intergroup comparison was done for all five parameters using Pearson correlation statistical analysis.

Results

EndoSequence BC sealer showed maximum mean of diameter of zones of inhibition against all the microorganisms but with no statistically significant difference with other sealers. Zical did not show any zone of inhibition against the P. aeruginosa. MM-Seal did not show any inhibitory activity against the P. aeruginosa and C. albicans.

Conclusion

EndoSequence BC sealer showed antimicrobial activity against all the microorganisms and proved to be a better choice when compared with other two sealers.

Clinical significance

Antimicrobial properties of endodontic sealers are important to prevent the persistent infection of the complex root canals. EndoSequence BC sealer has been proved to be a better sealer in this aspect.

How to cite this article

Singh G, Elshamy FMM, Homeida HE, Boreak N, Gupta I. An in vitro Comparison of Antimicrobial Activity of Three Endodontic Sealers with Different Composition. J Contemp Dent Pract 2016;17(7):553-556.


PDF Share
  1. Properties of novel resorbable chitosan calcium phosphate composites. Dent Mater 2003 Dec;19(8):797-804.
  2. Antibacterial activity of endodontic sealers by modified direct contact test against Enterococcus faecalis. J Endod 2009 Jul;35(7):1051-1055.
  3. Premix biological hydraulic cement paste composition and using the same. US patent application. 2,008,029,909. 2008 Dec 4.
  4. Antimicrobial activity and flow rate of newer and established root canal sealers. J Endod 2000 May;26(5):274-277.
  5. Influence of calcium hydroxide association on the physical properties of AH Plus. J Endod 2010 Jun;36(6):1048-1051.
  6. Antimicrobial effects of endodontic filling cements on microorganisms from root canals. Oral Surg Oral Med Oral Pathol 1983 Jun;55(6):622-627.
  7. Microbiological status of root-filled teeth with apical periodontitis. Int Endod J 1998 Jan;31(1):1-7.
  8. Microbiologic analysis of teeth with failed endodontic treatment and the outcome of conservative re-treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1998 Jan;85(1):86-93.
  9. Oral candidiasis: diagnosis and treatment. J Pract Hyg 2000 Sep;9:31-36.
  10. Occurrence of Candida albicans in infections of endodontic origin. J Endod 2000 Dec;26(12):695-698.
  11. Acute pulpal-alveolar cellulitis syndrome. III. Endodontic therapeutic factors and the resolution of a C. albicans infection. Oral Surg Oral Med Oral Pathol 1981 Dec;52(6):630-634.
  12. Fungi in endodontic infections. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2004 May;97(5):632-641.
  13. Diversity and frequency of yeasts from the dorsum of the tongue and necrotic root canals associated with primary apical periodontitis. Int Endod J 2009 Sep;42(9):839-844.
  14. Predominant obligate anaerobes invading the deep layers of root canal dentin. Int Endod J 1990 Jan;23(1):20-27.
  15. Bacteria recovered from teeth with apical periodontitis after antimicrobial endodontic treatment. Int Endod J 2003 Jul;36(7):500-508.
  16. Isolation of yeasts and enteric bacteria in root-filled teeth with chronic apical periodontitis. Int Endod J 2001 Sep;34(6):429-434.
  17. Monoinfection of root canal with Pseudomonas aeruginosa. Endod Dent Traumatol 1988 Dec;4(6):269-272.
  18. P. aeruginosa in oral infections. Acta Odont Scand 1972 Sep;30(3):371-381.
  19. Characterization of bacteria flora in persistent apical periodontitis lesions. Oral Microbiol Immunol 2009 Dec;24(6):502-505.
  20. In vitro evaluation of antimicrobial activity of sealers and pastes used in endodontics. J Endod 2000 Jul;26(7):391-394.
  21. Prevalence of selected microorganisms in the pulp space of human deciduous teeth with irreversible pulpitis. J Endod 2013;25(1):107-111.
  22. Refractory endodontic lesion associated with Staphylococci aureus. J Endod 1994 Dec;20(12):607-609.
  23. Antibacterial activity of potential retrograde root filling materials. Endod Dent Traumatol 1994 Apr;10(2):66-70.
  24. Two methods to evaluate the antimicrobial action of calcium hydroxide paste. J Endod 2001 Dec;27(12):720-723.
  25. Antibacterial activity of endosequence root repair material and ProRoot MTA against clinical isolates of Enterococcus faecalis. J Endod 2011 Nov;37(11):1542-1546.
  26. The rise of bioceramics in endodontics: a review. Int J Pharm Bio Sci 2015 Jan;6(1):416-422.
  27. Antifungal activity of endosequence root repair material and mineral trioxide aggregate. J Endod 2014 Nov;40(11):1815-1819.
  28. Biocompatibility of two novel root repair materials. J Endod 2011 Jun;37(6):793-798.
  29. Cytotoxicity comparison of mineral trioxide aggregates and endosequence bioceramic root repair materials. J Endod 2011 Mar;37(3):372-375.
  30. Antimicrobial effect of root canal cements on an obligate anaerobic organism. J Endod 1981 Jun;7(6):266-267.
  31. The bactericidal potential of various endodontic materials for primary teeth. Oral Surg Oral Med Oral Pathol 1978 Jun;45(6):947-954.
  32. Antibacterial properties of root canal sealers, cements and pastes. Int Endod J 1981 May;14(2):125-133.
  33. The pharmacologic and toxicological properties of zinc oxide-eugenol. J Am Dent Assoc 1986 Nov;113(5):781-791.
  34. Antibacterial properties of four endodontic sealers. J Endod 2008 Jun;34(6):735-738.
  35. Release of formaldehyde by 4 endodontic sealers. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1999 Aug;88(2):221-225.
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.