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VOLUME 18 , ISSUE 4 ( April, 2017 ) > List of Articles

RESEARCH ARTICLE

Ozone Gas Effect on Mineral Content of Dentin exposed to Streptococcus mutans Biofilm: An Energy-dispersive X-ray Evaluation

Carlos Estrela, Rafaella M Chaves, Paula C Cardoso, Terezinha de JE Barata, João B de Souza, Érica M de Torres, Cyntia RA Estrela, Ana PR Magalhães, Lawrence G Lopes

Citation Information : Estrela C, Chaves RM, Cardoso PC, de JE Barata T, de Souza JB, de Torres ÉM, Estrela CR, Magalhães AP, Lopes LG. Ozone Gas Effect on Mineral Content of Dentin exposed to Streptococcus mutans Biofilm: An Energy-dispersive X-ray Evaluation. J Contemp Dent Pract 2017; 18 (4):265-269.

DOI: 10.5005/jp-journals-10024-2029

Published Online: 01-04-2017

Copyright Statement:  Copyright © 2017; The Author(s).


Abstract

Aim

This study aims to assess the effect of ozone gas on dentin exposed to Streptococcus mutans biofilm by evaluation of mineral content [log calcium-to-phosphorus (Ca/P)] using energy-dispersive X-ray (EDX) spectroscopy.

Materials and methods

Five human third molars were sectioned into four slices of dentin and distributed in four groups: I – control (no treatment); II – ozone therapy; III – biofilm development; IV – ozone therapy followed by biofilm development. Mineral content (log Ca/P) was evaluated by EDX. Data were analyzed by analysis of variance and Tukey's test (p < 0.05).

Results

Results showed that the mineral content of control group (I) was similar to ozone group (II), and was statistically higher than biofilm (III) and ozone + biofilm (IV). The lowest log Ca/P was determined in biofilm group (III).

Conclusion

It can be concluded that ozone gas did not grant preventive effects of demineralization by S. mutans biofilm on dentin surface.

Clinical significance

Ozone gas therapy may be an alternative noninvasive treatment aiming to reduce the levels of caries-associated microorganisms. This therapy may, thereby, be an alternative and/or complementary treatment strategy in preventive dentistry.

How to cite this article

Chaves RM, Estrela C, Cardoso PC, de JE Barata T, de Souza JB, de Torres EM, Estrela CRA, Magalhães APR, Lopes LG. Ozone Gas Effect on Mineral Content of Dentin exposed to Streptococcus mutans Biofilm: An Energy-dispersive X-ray Evaluation. J Contemp Dent Pract 2017;18(4):265-269.


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