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VOLUME 18 , ISSUE 11 ( November, 2017 ) > List of Articles

RESEARCH ARTICLE

Histological Evaluation of the Effect of Platelet-rich Plasma on Pulp Regeneration in Nonvital Open Apex Teeth: An Animal Study

Maryam Forghani, Hamid Jafarzadeh, Jamileh Ghoddusi, Amir Maghsudlu, Amirhossein Jafarian

Citation Information : Forghani M, Jafarzadeh H, Ghoddusi J, Maghsudlu A, Jafarian A. Histological Evaluation of the Effect of Platelet-rich Plasma on Pulp Regeneration in Nonvital Open Apex Teeth: An Animal Study. J Contemp Dent Pract 2017; 18 (11):1045-1050.

DOI: 10.5005/jp-journals-10024-2173

Published Online: 01-11-2017

Copyright Statement:  Copyright © 2017; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Aim

Platelet-rich plasma (PRP), which is a concentration of growth factors found in platelets, may be a suitable material for pulp regeneration. The aim of this animal study was a histological evaluation of PRP on pulp regeneration in nonvital teeth with immature apices.

Materials and methods

A total of 40 premolar dogs’ teeth were chosen for this study. After general anesthesia, the teeth were exposed, and subsequently, pulps were removed and the cavities were opened to the oral cavity. After 2 weeks, root canals were irrigated and disinfected with sodium hypochlorite with noninstrumentation technique, and triple antibiotic paste was placed inside the canals. Cavities were sealed with a temporary restoration. About 4 weeks later, canals were irrigated again and the teeth were randomly divided into three groups. Bleeding was evoked with overinstrumentation, then experimental materials for each group [PRP, mineral trioxide aggregate (MTA), and parafilm respectively] were placed over the bleeding, and orifices were sealed with MTA and glass ionomer. After 3 months, dogs were sacrificed and the teeth were separated from the jaws and sections prepared for histological evaluation.

Results

Regeneration was shown in 44.7% of the samples. About 47.3% of the samples in the MTA group and 42.1% of the samples in the PRP group showed regeneration; however, no regeneration was observed in the parafilm group. Chi-square test showed no significant difference between groups I and II. The soft regenerative tissue included pulp-like tissue and vessels. Mineralized regenerative tissue included cementumlike, periodontal ligament-like, and bone-like tissues. No normal pulp and nerve tissue were observed.

Conclusion

Both PRP and MTA may be ideal scaffolds to accelerate the regeneration process.

Clinical significance

Pulp repair in immature permanent teeth with weak roots has a better outcome than replacement of the pulp with gutta-percha or biomaterials.

How to cite this article

Ghoddusi J, Maghsudlu A, Jafarzadeh H, Jafarian A, Forghani M. Histological Evaluation of the Effect of Platelet-rich Plasma on Pulp Regeneration in Nonvital Open Apex Teeth: An Animal Study. J Contemp Dent Pract 2017;18(11):1045-1050.


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  1. Revitalization of tooth with necrotic pulp and open apex by using platelet-rich plasma: a case report. J Endod 2011 Feb;37(2):265-268.
  2. Vital pulp therapy-current progress of dental pulp regeneration and revascularization. Int J Dent 2010 Feb;2010:856087.
  3. ; Trowbridge, HO.; Rafter, M. Protecting the pulp, preserving the apex. In: Torabinejad M, Walton RE, editors. Endodontics, principles and practice. 4th ed. Philadelphia (PA): Saunders; 2009.
  4. Repair and regeneration in endodontics. Int Endod J 2011 Oct;44(10):889-906.
  5. A retrospective evaluation of radiographic outcomes in immature teeth with necrotic root canal systems treated with regenerative endodontic procedures. J Endod 2009 Oct;35(10):1343-1349.
  6. ; Cohen, S. Cohen's pathways of the pulp. 10th ed. St. Louis (MO): Mosby; 2011.
  7. Pulp revascularization of immature teeth with apical periodontitis: a clinical study. J Endod 2009 May;35(5):745-749.
  8. Revascularization with and without platelet-rich plasma in nonvital, immature, anterior teeth: a pilot clinical study. J Endod 2012 Dec;38(2):1581-1587.
  9. Revascularization of immature permanent teeth with apical periodontitis: new treatment protocol? J Endod 2004 Apr;30(4):196-200.
  10. The plasma-rich in growth factor as a suitable matrix in regenerative endodontics: a case series. N Y State Dent J 2014 Jun-Jul;80(4):49-53.
  11. Periodontal healing of replanted dog teeth stored in milk and egg albumen. Dent Traumatol 2008 Oct;24(5):510-514.
  12. Challenges in regenerative endodontics: a case series. J Endod 2010 Mar;36(3):536-541.
  13. Pulp revascularization of immature dog teeth with apical periodontitis. J Endod 2007 Jun;33(6):680-689.
  14. Understanding and evaluating platelet function. Hematology Am Soc Hematol Educ Program 2010;2010:387-396.
  15. Reaction of rat connective tissue to polyethylene tube implants. II. Oral Surg Oral Med Oral Pathol 1967 Nov;24(5):674-683.
  16. Effect of platelet-rich plasma on bone growth and osseointegration in human maxillary sinus grafts: three bilateral case reports. Int J Periodontics Restorative Dent 2002 Feb;22(1):45-53.
  17. Effect of platelet-rich plasma on bone regeneration in autogenous bone graft. Int J Oral Maxillofac Surg 2004 Jan;33(1):56-59.
  18. Mineral trioxide aggregate: a comprehensive literature review – part II: leakage and biocompatibility investigations. J Endod 2010 Feb;36(2):190-202.
  19. Cementum and periodontal wound healing and regeneration. Crit Rev Oral Biol Med 2002;13(6):474-484.
  20. Molecular factors regulating development and regeneration of cementum. J Periodontal Res 1993 Nov;28(6 Pt 2):550-559.
  21. Growth factors for bone growth and repair: IGF, TGF beta and BMP. Bone 1996 Jul;19(1 Suppl):1S-12S.
  22. Mineral trioxide aggregate induces bone morphogenetic protein-2 expression and calcification in human periodontal ligament cells. J Endod 2010 Apr;36(4):647-652.
  23. ; Corr, R.; Shabahng, S.; Torabinejad, M. Identification of tissues formed after revascularization in a ferret model. Poster, AAE Annual Session; 2011.
  24. Immature teeth with periradicular periodontitis or abscess undergoing apexogenesis: a paradigm shift. J Endod 2006 Dec;32(12):1205-1213.
  25. Revascularization and periapical repair after endodontic treatment using apical negative pressure irrigation versus conventional irrigation plus triantibiotic intracanal dressing in dogs' teeth with apical periodontitis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010 May;109(5):779-787.
  26. Revitalization of pulpless open apex teeth in rhesus monkeys, using collagen-calcium phosphate gel. J Endod 1976 Jun;2(6):159-165.
  27. Histologic characterization of regenerated tissues in canal space after the revitalization/revascularization procedure of immature dog teeth with apical periodontitis. J Endod 2010 Jan;36(1):56-63.
  28. Tissue engineering strategies for immature teeth with apical periodontitis. J Endod 2011 Mar;37(3):390-397.
  29. Periodontal cell migration into the apical pulp during the repair process after pulpectomy in immature teeth: an autoradiographic study. J Oral Rehabil 1993 Nov;20(6):637-652.
  30. Stem cells and periodontal regeneration. Periodontol 2000 2006 Feb;40(1):164-172.
  31. Investigation of multipotent postnatal stem cells from human periodontal ligament. Lancet 2004 Jul;364(9429):149-155.
  32. Regenerative treatment of an immature, traumatized tooth with apical periodontitis: report of a case. J Endod 2008 May;34(5):611-616.
  33. Pulpal changes in replanted and autotransplanted immature teeth of dogs. J Endod 1981 Jul;7(7):309-316.
  34. Regenerative endodontic treatment for necrotic immature permanent teeth. J Endod 2009 Feb;35(2):160-164.
  35. Biologically based treatment of immature permanent teeth with pulpal necrosis: a case series. J Endod 2008 Jul;34(7):876-887.
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