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VOLUME 18 , ISSUE 11 ( November, 2017 ) > List of Articles


Histological Evaluation of the Effect of Platelet-rich Plasma on Pulp Regeneration in Nonvital Open Apex Teeth: An Animal Study

Maryam Forghani, Hamid Jafarzadeh, Jamileh Ghoddusi, Amir Maghsudlu, Amirhossein Jafarian

Citation Information : Forghani M, Jafarzadeh H, Ghoddusi J, Maghsudlu A, Jafarian A. Histological Evaluation of the Effect of Platelet-rich Plasma on Pulp Regeneration in Nonvital Open Apex Teeth: An Animal Study. J Contemp Dent Pract 2017; 18 (11):1045-1050.

DOI: 10.5005/jp-journals-10024-2173

Published Online: 01-09-2016

Copyright Statement:  Copyright © 2017; The Author(s).



Platelet-rich plasma (PRP), which is a concentration of growth factors found in platelets, may be a suitable material for pulp regeneration. The aim of this animal study was a histological evaluation of PRP on pulp regeneration in nonvital teeth with immature apices.

Materials and methods

A total of 40 premolar dogs’ teeth were chosen for this study. After general anesthesia, the teeth were exposed, and subsequently, pulps were removed and the cavities were opened to the oral cavity. After 2 weeks, root canals were irrigated and disinfected with sodium hypochlorite with noninstrumentation technique, and triple antibiotic paste was placed inside the canals. Cavities were sealed with a temporary restoration. About 4 weeks later, canals were irrigated again and the teeth were randomly divided into three groups. Bleeding was evoked with overinstrumentation, then experimental materials for each group [PRP, mineral trioxide aggregate (MTA), and parafilm respectively] were placed over the bleeding, and orifices were sealed with MTA and glass ionomer. After 3 months, dogs were sacrificed and the teeth were separated from the jaws and sections prepared for histological evaluation.


Regeneration was shown in 44.7% of the samples. About 47.3% of the samples in the MTA group and 42.1% of the samples in the PRP group showed regeneration; however, no regeneration was observed in the parafilm group. Chi-square test showed no significant difference between groups I and II. The soft regenerative tissue included pulp-like tissue and vessels. Mineralized regenerative tissue included cementumlike, periodontal ligament-like, and bone-like tissues. No normal pulp and nerve tissue were observed.


Both PRP and MTA may be ideal scaffolds to accelerate the regeneration process.

Clinical significance

Pulp repair in immature permanent teeth with weak roots has a better outcome than replacement of the pulp with gutta-percha or biomaterials.

How to cite this article

Ghoddusi J, Maghsudlu A, Jafarzadeh H, Jafarian A, Forghani M. Histological Evaluation of the Effect of Platelet-rich Plasma on Pulp Regeneration in Nonvital Open Apex Teeth: An Animal Study. J Contemp Dent Pract 2017;18(11):1045-1050.

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