In situ Effect of Nanohydroxyapatite Paste in Enamel Teeth Bleaching
Cecy Martins Silva, Yasmin SB de Lima Gomes, Larissa D Alexandrino, Cristiane de M Alencar, Eliane B Alves, Kelson CF Faial
Citation Information :
Silva CM, de Lima Gomes YS, Alexandrino LD, de M Alencar C, Alves EB, Faial KC. In situ Effect of Nanohydroxyapatite Paste in Enamel Teeth Bleaching. J Contemp Dent Pract 2017; 18 (11):996-1003.
Evaluate in situ the effect of nanohydroxyapatite paste (nano-HAP) before bleaching with hydrogen peroxide 35% (HP35%) by ion chromatography (IC) Knoop hardness number (KHN) and tristimulus colorimetry (TC).
Materials and methods
A total of 60 fragments were obtained from third molars included (3 mm × 3 mm × 3 mm) and the specimens were divided into three groups (n = 20): Gas chromatography (CG) (negative control group) = no bleaching; HP35% (positive control group) = HP35% whitening (whiteness HP35%); nano-HAP = application for 10 minutes before bleaching treatment + HP35%. The specimens were fixed to the volunteers’ molars. The KHN and TC were measured before and after bleaching. For IC, the dentin layer was removed, leaving the enamel that was crushed, and autoclaved for chemical quantification (calcium, fluorine, and phosphorus). The results of KHN and TC were analyzed statistically by analysis of variance (ANOVA) followed by Tukey test (p < 0.05).
The HP35% group showed reduction of the Ca, F, and P ions. The initial and final KHN mean of the CG and nano-HAP did not differ statistically; however, the group of HP35% did differ statistically. The mean ∆E of the HP35% and nano-HAP groups did not differ statistically from each other. However, they differed from the CG.
The nano-HAP paste preserved the KHN, promoted the lower loss of Ca and P ions and an increase of F ions when compared with the CG, but did not influence the effectiveness of the bleaching treatment.
Nano-HA is a biomaterial that has shown positive results in the prevention of deleterious effects on the enamel by the action of the office bleaching treatment.
How to cite this article
Gomes YSBL, Alexandrino LD, Alencar CDM, Alves EB, Faial KCF, Silva CM. In situ Effect of Nanohydroxyapatite Paste in Enamel Teeth Bleaching. J Contemp Dent Pract 2017;18(11):996-1003.
In vitro FT-IR study of the effects of hydrogen peroxide on superficial tooth enamel. J Dent 2007 Apr;35(4):325-330.
Effects of bleaching agents containing fluoride and calcium on human enamel. Quintessence Int 2010 Sep;41(8):e157-e165.
Influence of study design on the impact of bleaching agents on dental enamel microhardness: a review. Dent Mater 2009 Feb;25(2):143-157.
Effect of 10% and 15% carbamide peroxide on fracture toughness of human dentin in situ. Oper Dent 2013 Mar-Apr;38(2):142-150.
Hidroxiapatita: Obtenção, caracterização e aplicações. Rev Eletr Mat Proc 2009;4(3):29-38.
Effect of 7.5% hydrogen peroxide containing remineralizing agents on hardness, color change, roughness and micromorphology of human enamel. Am J Dent 2015 Oct;28(5):261-267.
Do different bleaching protocols affect the enamel microhardness? Eur J Dent 2015 Jan-Mar;9(1):25-30.
In situ and in vitro effects of bleaching with carbamide peroxide on human enamel. Oper Dent 2004 Mar-Apr;29(2):219-225.
Influence of the duration of treatment using a 10% carbamide peroxide bleaching gel on dentin surface microhardness: an in situ study. Quintessence Int 2005 Jan;36(1):15-24.
Effect of hydrogen peroxide at 35% on the morphology of enamel and interference in the de-remineralization process: an in situ study. Oper Dent 2012 Sep-Oct;37(5):518-525.
In situ caries models. Adv Dent Res 1995 Nov;9(3):214-230.
Measuring salivary flow: challenges and opportunities. J Am Dent Assoc 2008 May; 139 (Suppl):35S-40S.
Velocidade do fluxo salivar e pH salivar após radioterapia da região de cabeça e pescoço. Rev Bras Cancerol 2004 Sep;50(4):287-293.
Quantitative assessment for stimulated saliva flow rate and buffering capacity in relation to different ages. J Dent 2006 Oct;34(9):716-720.
Nanocomposites of hydroxyapatite with aspartic acid and glutamic acid and their interaction with osteoblast-like cells. Biomaterials 2006 Sep; 27(25):4428-4433.
Beneficial effects of hydroxyapatite on enamel subjected to 30% hydrogen peroxide. J Dent 2008 Nov; 36(11):907-914.
Environmental applications of ion chromatography in Eastern and Central Europe. J Chromatogr Sci 2010 Aug; 48(7):559-565.
Effect of nano carbonate apatite to prevent re-stain after dental bleaching in vitro. J Dent 2011 Sep;39(9):636-642.
Crystallization process of carbonate substituted hydroxyapatite nanoparticles in toothpastes upon physiological conditions: an in situ time-resolved X-ray diffraction study. J Mater Sci Mater Med 2010 Feb;21(2):445-450.
Comparison of nano-hydroxyapatite and sodium fluoride mouthrinse for remineralization of incipient carious lesions. J Dent (Tehran) 2014 Jul; 11(4):406-410.
Material fundamentals and clinical performance of plasma-sprayed hydroxyapatite coatings: a review. J Biomed Mater Res 2001 Oct;58(5):570-592.
The influence of fluoride on the seeded growth of apatite from stable supersaturated solutions at pH 7.4. J Dent Res 1980 Feb;59(2):144-150.
Lead immobilization from aqueous solutions and contaminated soils using phosphate rocks. Environ Sci Technol 1995 Apr;29(4):1118-1126.
The effects of anti-oxidant agents as neutralizers of bleaching agents on enamel bong strength. Braz J Oral Sci 2006;5(16):971-976.
Effect of nano-hydroxyapatite concentration on remineralization of initial enamel lesion in vitro. Biomed Mater 2009 Jun;4(3):034104.
Effect of in-office bleaching with 35% hydrogen peroxide with and without addition of calcium on the enamel surface. Microsc Res Tech 2015 Aug;78(11):975-981.
One-year clinical evaluation of the efficacy of a new daytime at-home bleaching technique. J Esthet Restor Dent 2010 Apr; 22(2):139-146.
Principles of color technology. New York: John Wiley & Sons; 1981.
Intraoral determination of the tolerance of dentists for perceptibility and acceptability of shade mismatch. J Prosthet Dent 2007 Apr;97(4):200-208.
Effect of light irradiation on tooth whitening: Enamel microhardness and color change. J Esthet Restor Dent 2009 Dec; 21(6):387-394.
Tooth bleaching by different concentrations of carbamide peroxide and hydrogen peroxide whitening strips: an in vitro study. J Esthet Restor Dent 2006 Feb; 18(2):93-100.
Tooth whitening with hydrogen/carbamide peroxides in association with a CPP-ACP paste at different proportions. Aust Dent J 2012 Jun;57(2):213-219.
Tooth whitening: tooth color changes following treatment by peroxide and light. J Clin Dent 2005;16(3):78-82.
Hydrogen peroxide whitens teeth by oxidizing the organic structure. J Dent 2012 Dec;40(Suppl 2):e25-e33.
Changes in bovine enamel after treatment with a 30% hydrogen peroxide bleaching agent. Dent Mater J 2004 Dec;23(4):517-521.
In vitro evaluation of the efficacy of different over-the-counter products on tooth whitening. Braz Dent J 2015 Jul-Aug;26(4):373-377.
Opalescence of bleached teeth. J Dent 2012 Jul;40(Suppl 1):e35-e39.
Chemical alteration by tooth bleaching of human salivary proteins that infiltrated subsurface enamel lesions – experimental study with bovine lesion model systems. Dent Mater J 2014;33(5):663-668.
Effects of two in-office bleaching agents with different pH on the structure of human enamel: An in situ and in vitro study. Oper Dent 2013 Jan-Feb;38(1):100-110.
Effects of pH values of hydrogen peroxide bleaching agents on enamel surface properties. Oper Dent 2011 Aug; 36(5):554-562.
Safety issues of tooth whitening using peroxide-based materials. Br Dent J 2013 Jul; 215(1):29-34.
Effectiveness of different carbamide peroxide concentrations used for tooth bleaching: an in vitro study. J Appl Oral Sci 2012 Mar-Apr;20(2):186-191.