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VOLUME 18 , ISSUE 12 ( December, 2017 ) > List of Articles


Impact of Mouthwashes on Antibacterial Activity of Subjects with Fixed Orthodontic Appliances: A Randomized Clinical Trial

A Nishad, NS Sreesan, Joseph Joy, Lakshmi Lakshmanan, Joyce Thomas, VA Anjali

Citation Information : Nishad A, Sreesan N, Joy J, Lakshmanan L, Thomas J, Anjali V. Impact of Mouthwashes on Antibacterial Activity of Subjects with Fixed Orthodontic Appliances: A Randomized Clinical Trial. J Contemp Dent Pract 2017; 18 (12):1112-1116.

DOI: 10.5005/jp-journals-10024-2185

Published Online: 01-06-2017

Copyright Statement:  Copyright © 2017; The Author(s).



The study aimed to assess the impact of mouthwashes on antibacterial activity of individuals with fixed orthodontic appliances.

Materials and methods

A total of 60 individuals were considered in the study. Sixty (20 each group) nonextraction class I individuals were randomly divided into experimental and control groups. Group I: Experimental group [chlorhexidine (CHX) mouthwash], group II: Experimental group (neem mouthwash), group III: Control group (distilled water). All the clinical examinations were done at baseline and 30th day respectively, after the start of orthodontic treatment. The mean differences between the different experimental groups were calculated using one-way analysis of variance (ANOVA) test.


There was no statistical significance at baseline mean plaque index (PI), gingival index (GI) scores, and Streptococcus mutans (SM) colony count between groups. The PI and GI scores among CHX and neem mouthwash groups (p = 0.002, p = 0.032 respectively) were significantly reduced after intervention and also the SM colonies count was reduced in CHX and neem mouthwash groups and there was significant difference between the groups.


As both mouthwashes showed significant effectiveness on antibacterial activity in individuals with fixed orthodontic appliances, neem mouthwash can be used as an alternative to CHX.

Clinical significance

It is better to have a sound knowledge regarding the use of mouthwash in long term as fixed orthodontics are associated with accumulation of SM, enamel demineralization, and an increased number of carious lesions, predominantly in sites adjacent to bracket.

How to cite this article

Nishad A, Sreesan NS, Joy J, Lakshmanan L, Thomas J, Anjali VA. Impact of Mouthwashes on Antibacterial Activity of Subjects with Fixed Orthodontic Appliances: A Randomized Clinical Trial. J Contemp Dent Pract 2017;18(12):1112-1116.

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