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VOLUME 10 , ISSUE 2 ( March, 2009 ) > List of Articles


Microleakage of an Adhesive System used as a Fissure Sealant

José Carlos Pettorossi Imparato, Daniela Prócida Raggio, Clarissa Calil Bonifacio, Ricardo Scarparo Navarro, Fernanda Sardenberg, Rubens Corte Real de Carvalho

Citation Information : Imparato JC, Raggio DP, Bonifacio CC, Navarro RS, Sardenberg F, de Carvalho RC. Microleakage of an Adhesive System used as a Fissure Sealant. J Contemp Dent Pract 2009; 10 (2):26-33.

DOI: 10.5005/jcdp-10-2-26

License: CC BY-NC 3.0

Published Online: 01-06-2011

Copyright Statement:  Copyright © 2009; The Author(s).



The aim of this study was to investigate the microleakage of pit and fissure sealants in primary teeth and to determine if multi-step methods should produce better results in terms of microleakage prevention.

Methods and Materials

A total of 40 intact primary molars were randomly assigned to one of four sealant groups: resin based sealant Ultraseal XT (US – Ultradent) (Group 1, n=10); adhesive system Scotchbond Multi Purpose Plus (SBMP - 3M/ESPE) used as a sealant (Group 2, n=10); primer (SBMP) and US (Group 3, n=10); and primer, bond (SBMP) and US (Group 4, n=10). After thermocycling (x700, 5-55°C) all teeth surfaces were made impermeable with the exception of the occlusal surface. Samples were immersed in 50% silver nitrate for 8 hours, sectioned longitudinally with two cuts in the central fissure pit, and then immersed in photo development solution followed by 16 hours in fluorescent light. Pictures were obtained by light microscope (x40) and the degree of microleakage was evaluated by three blinded evaluators. The data were analyzed using the Kruskal Wallis test.


Significant statistical differences (p<.05) were observed between Groups 1-3, Groups 2-3, Groups 2-4, and Groups 3-4. Group 3 showed higher microleakage scores.


The adhesive system and the resin based sealant demonstrated similar microleakage properties, suggesting the possibility that adhesives can be used in a clinical setting. Additionally, the increase in the number of clinical steps required by common application of resin based sealant, including use of primer or primer and bond, did not lead to better results in preventing microleakage.

Clinical Significance

The adhesive system can be used as a sealant without increasing the microleakage. Thus, the use of the adhesive system before the application of the sealant is not necessary to reduce the microleakage, although further research is warranted to support this conclusion.


Bonifacio CC, Navarro RS, Sardenberg F, Imparato JCP, Carvalho RCR, Raggio DP. Microleakage of an Adhesive System used as a Fissure Sealant. J Contemp Dent Pract 2009 March; (10)2:026-033.

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