Periodontal Bacterial Load: A Proposed New Epidemiological Method for Periodontal Disease Assessment
Magda Feres, Luciene Cristina de Figueiredo, Mario Vianna Vettore, Anna Thereza Thomé Leão, Maria do Carmo Leal, Aubrey Sheiham
Citation Information :
Feres M, de Figueiredo LC, Vettore MV, Leão AT, Leal MD, Sheiham A. Periodontal Bacterial Load: A Proposed New Epidemiological Method for Periodontal Disease Assessment. J Contemp Dent Pract 2010; 11 (1):49-56.
The purpose of this study was to identify a periodontal clinical measure that correlates with red complex bacteria usually associated with periodontal disease.
Methods and Materials
Periodontal clinical parameters were recorded in 116 postpartum women at six sites per tooth for all teeth excluding third molars. Two subgingival plaque samples per subject were collected and analyzed for 39 bacterial species using the Checkerboard DNADNA hybridization technique. Periodontal Bacterial Load (PBL) was calculated as the sum of all pocket depth measurements of 4 mm at sites with a Clinical Attachment Level (CAL) of 4 mm. The association of clinical and bacterial scores was analyzed using the Spearman correlation coefficient and the Kruskal-Wallis test.
Results
The PBL was correlated with microorganisms from the red complex that included Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola, individually or grouped (p<0.05). The PBL was not associated with periodontally beneficial species from the yellow, green, purple, and blue complexes (p>0.05). The proportions and mean counts of the red complex were increased according to the quartile groups of distribution of the PBL.
Conclusions
PBL appears to be a reliable measure of periodontal status in postpartum women.
Clinical Significance
PBL avoids bias in the assessment of periodontal status in studies of periodontal disease.
Citation
Vettore MV, Leão ATT, Leal MC, Feres M, Figueiredo LC, Sheiham A. Periodontal Bacterial Load: A Proposed New Epidemiological Method for Periodontal Disease Assessment. J Contemp Dent Pract [Internet]. 2010 Jan; 11(1):049-056. Available from: http://www.thejcdp. com/journal/view/volume11-issue1-vettore.
A system of classification and scoring for prevalence surveys of periodontal disease. J Dent Res. 1956; 35(3):350-9.
Indices for prevalence and incidence of periodontal disease. J Periodontol. 1959; 30:51-9.
Oral Health Surveys: Basic Methods, 4th ed. Geneva: WHO; 1997. 73 p.
The extent and severity index: a simple method for use in epidemiologic studies of periodontal disease. J Clin Periodontol. 1986;13(5):500-5.
Gingival recession, gingival bleeding, and dental calculus in adults 30 years of age and older in the United States, 1988–1994. J Periodontol. 1999; 70(1):30-43.
Periodontal infections and pre-term low birth weight: a case-control study. J Clin Periodontol. 2005; 32(2):174-81.
A prospective study to investigate the relationship between periodontal disease and adverse pregnancy outcome. Br Dent J. 2004; 197(5):251-8.
Higher risk of preterm birth and low birth weight in women with periodontal disease. J Dent Res. 2002; 81(1):58-63.
Periodontal infections as a possible risk factor for preterm low birth weight. J Periodontol. 1996; 67(10 Suppl):1103-13.
A possible association between preterm birth and early periodontitis. A pilot study. J Clin Periodontol. 2004; 31(9):736-41.
Periodontal infection and undesirable pregnancy outcomes: a systematic review of epidemiologic studies. Cad Saúde Pública. 2006; 22(10):2041-53.
New concepts of destructive periodontal disease. J Clin Periodontol. 1984; 11(1):21-32.
Microbial complexes in subgingival plaque. J Clin Periodontol. 1998; 25(2):134-44.
Differences in the subgingival microbiota of Swedish and USA subjects who were periodontally healthy or exhibited minimal periodontal disease. J Clin Periodontol. 2005; 32(1):33-9.
Quantitative detection of periodontal pathogens using real-time polymerase chain reaction with TaqMan probes. Oral Microbiol Immunol. 2004; 19(3):168-76.
The predominant cultivable microbiota of active and inactive lesions of destructive periodontal diseases. J Clin Periodontol. 1988; 15(5): 316-23.
The relationship between periodontitis and preterm low birthweight. J Dent Res. 2008; 87(1):73-8.
Clinical evaluation of periodontal diseases. Periodontol 2000. 1995; 7:39-53.
Problems and proposals for recording gingivitis and plaque. Int Dent J. 1975; 25(4):229-35.
The effect of SRP on the clinical and microbiological parameters of periodontal diseases. J Clin Periodontol. 1997; 24(5):324-34.
