The aim of this study was to assess the periodontal status in subjects with hyperlipidemia and to determine whether there is any association between hyperlipidemia and periodontitis.

Sixty subjects with hyperlipidemia and 60 normolipidemic subjects participated in the study. Biochemical parameters assessed included plasma triglyceride, total cholesterol, low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL-C) levels. Periodontal parameters assessed included plaque index (PI), probing depth (PD), clinical attachment level (CAL) and percentage of sites with bleeding on probing (BOP).

Hyperlipidemic patients showed significantly higher values across the four periodontal parameters. Plasma triglycerides, total cholesterol, LDL-C were significantly and positively associated with PD, BOP and CAL (p = 0.05). Whereas HDL-C levels were significantly and negatively associated with PD, BOP and CAL (p = 0.05).

Patients with hyperlipidemia manifested higher values of periodontal parameters compared to control group. Poorly controlled hyperlipidemia had a destructive impact on periodontium. A cyclic relationship exists between serum lipids, periodontitis and systemic health.

The relationship between periodontium and systemic factors is bidirectional and interlinked. Therefore, dentists and physicians should take this into account while treating patients, aiming for the overall betterment of the patient.

Shivakumar TP, Patil VA, Desai MH. Periodontal Status in Subjects with Hyperlipidemia and Determination of Association between Hyperlipidemia and Periodontal Health: A Clinicobiochemical Study. J Contemp Dent Pract 2013;14(5):785-789.

The aim of the present study was to investigate the effectiveness of the one total-etch self-priming adhesive, one two-step self-etching primer adhesive, and one ‘all-in-one’ selfetching adhesive system on the adhesion of a resin composite to enamel.

Thirty-six freshly extracted human mandibular molars were selected for this study. A flat area about 5 mm in diameter was created on the exposed mesial surface of enamel of each tooth by moist grinding with 320, 420 and 600 grit silicon carbide paper. Twelve teeth were randomly assigned into three groups. In group 1, Adper Easy One (3M ESPE), a one step self-etching primer adhesive was applied and light curing unit for 10 seconds. In group 2, Adper SE Plus, a twostep self-etching primer with bottle A containing the aqueous primer and bottle B containing the acidic adhesive was applied and light cured for 10 seconds. Group 3 (control)—etchant 37% phosphoric acid is applied to the surface for 15 seconds and rinsed with water and air dried and adhesive (single bond 2) is applied to the surface and tube is placed and light cured for 20 seconds. Composite material (Z350) was placed in the tube and light cured for 40 seconds in all the groups.

Bond strength testing was done using universal testing machine at the enamel-composite interface. The debonded enamel surface was evaluated in stereomicroscope to assess the cohesive, adhesive or mixed fracture. Data was statistically analyzed by one way analysis of variance (ANOVA).

Group 1 performed least among all groups with a mean score of 19.46 MPa. Group 2 had a mean score of 25.67 MPa. Group 3 had a mean score of 27.16 MPa.

Under the conditions of this in vitro study, the bond strength values of the two-step self-etching primer systems tested were similar to the total-etch. And, one step self-etching primers have lower bond strength compared to the total-etch.

Patil BS, Rao BKR, Sharathchandra SM, Hegde R, Kumar GV. Comparative Evaluation of Selfetching Primers and Phosphoric Acid Effectiveness on Composite to Enamel Bond: An in vitro Study. J Contemp Dent Pract 2013;14(5):790-795.

This study aims to compare the clot stabilization on root surfaces conditioned with citric acid and ethylenediaminetetraacetic acid (EDTA).

Scaled root samples (n = 100) were set in five groups: group I–control group (saline solution); group II (24% EDTA); group III (25% citric acid); group IV (EDTA + citric acid); group V (citric acid + EDTA). Fifty samples were assessed using the root surface modification index (RSMI). The other 50 received a blood drop after conditioning. Clot formation was assessed using blood elements adhesion index (BEAI). A blind examiner evaluated photomicrographs. Statistical analysis considered p < 0.05.

Groups-III and G-V attained the best results for RSMI and BEAI in comparison to control. The worst results for clot stabilization were seen in group-II. EDTA employment before citric acid (group-IV) reduced clot formation in comparison to citric acid use alone (group-III).

Root conditioning with citric acid alone and before EDTA had the best results for smear layer removal and clot stabilization. EDTA inhibited clot stabilization on root surface and must have a residual activity once it has diminished clot adhesion to root even after citric acid conditioning. Thus, EDTA can be used to neutralize citric acid effects on periodontal cells without affecting clot stabilization.

To demonstrate that citric acid use on root surfaces previously affected by periodontal disease may favor clot stabilization and may have a beneficial effect on surgical outcomes. Also, EDTA can be used to neutralize citric acid effects on periodontal cells.

Leite FRM, Nascimento GG, Leite ERM, Leite AA, Sampaio JEC. Effect of the Association between Citric Acid and EDTA on Root Surface Etching. J Contemp Dent Pract 2013;14(5):796-800.

Biochemical derangements of microelements although reported in oral cancer is poorly understood in oral submucous fibrosis (OSMF). Hence, the present study was carried out to estimate and compare the serum copper and zinc levels among different histopathological stages of OSMF with that of healthy controls.

Thirty histopathologically diagnosed cases of OSMF and 30 healthy controls reporting to MS Ramaiah Dental College and Hospital (MSRDCH), Bengaluru, were included in the study. The histopathological staging of OSMF was done as per criteria given by Pindborg and Sirsat (1966). Blood samples were collected and the serum copper and zinc levels were estimated using atomic absorption spectroscopy. The results were statistically analyzed using independent t-test and Kruskal-Wallis test.

The mean serum copper levels were increased (p < 0.0005) and zinc levels were decreased (p < 0.01) in OSMF patients when compared to that of controls. The mean serum copper levels showed an increasing trend from stage I to IV (p = 0.004) while zinc levels showed a decreasing trend from stage I to IV (p = 0.04) in OSMF patients.

The serum copper and zinc levels in OSMF showed a significant difference in comparison to that of controls and between the histopathological stages. These findings indicate that serum copper and zinc may have a contributory role in the etiopathogenesis of OSMF.

Although the role of the trace elements as etiological factors is minor, derangements noted in the serum copper and zinc levels could be correlated with diseased progression and possibly explain the transformation of OSMF into malignancy. Thus, they can be used as prognostic markers and can be of value for proactive intervention.

Neethi H, Patil S, Rao RS. Estimation of Serum Copper and Zinc Levels in Oral Submucous Fibrosis: An Atomic Absorption Spectroscopic Study. J Contemp Dent Pract 2013;14(5):801-805.

The aim of this study was to establish the validity of a new method for evaluating skeletal maturation by assessing the 3rd and 4th cervical vertebrae seen in the cephalometric radiograph.

This study consisted of a sample of 50 patients in the age group of 8 to 14 years of age. Chronologically, they were divided into six groups, based on the age consisting of a minimum of six to a maximum of 10 subjects. All the patients included in the study were females. The selected subjects were clinically examined and then age and date of birth of the patient in years and months was noted. Then lateral cephalograms and hand-wrist radiographs of the patient were taken on the same day with good clarity and contrast.

The results suggested that cervical vertebral bone age on cephalometric radiographs calculated with this method is as reliable at estimating bone age as is the Tanner-Whitehouse 3 (TW3) method on hand-wrist radiographs. By determining the cervical vertebral bone age, skeletal maturity can be evaluated in a detailed and objective manner with cephalometric radiographs.

The ability to accurately appraise skeletal maturity from cervical vertebral maturation, without the need for additional radiographs, has the potential to improve orthodontic diagnostic and therapeutic decisions. The technique's simplicity and ease of use should encourage this method as a first level diagnostic tool to assess skeletal maturation.

This study revealed that the timing and sequence of ossification of the bones in hand and wrist and cervical vertebrae were able to relate the skeletal development of the various skeletal maturity indicators to a child's development. This method provided a mean with which one can determine the skeletal maturity of a person and thereby determine whether the possibility of potential growth existed.

Prasad CMSK, Reddy VN, Sreedevi G, Ponnada SR, Priya KP, Naik BR. Objective Evaluation of Cervical Vertebral Bone Age—Its Reliability in Comparison with Hand-Wrist Bone Age: By TW3 Method. J Contemp Dent Pract 2013;14(5):806-813.

To evaluate and compare the serum IgM level in diabetic and nondiabetic patients with chronic periodontitis.

A total of 60 patients were selected for the study and divided into four groups, diabetic with periodontitis, diabetic without periodontitis, nondiabetic with periodontitis and nondiabetic without periodontitis (control) were analyzed for the quantitative estimation of serum immunoglobulins M by turbidimetric immunoassay. The serum of the diabetic and nondiabetic patients was evaluated and turbidimetric method was used for immunological assay by using Quantia IgM turbidimetric immunoassay for estimation of immunoglobulin IgM in human serum. The data for the level of immunoglobulin thus obtained were compared with clinically healthy patient taken as control. Sugar level was estimated the by checking the random blood sugar level by glucose test kit based on end point and kinetic assay and compared with the HbA1c percentage of the patients, by using NycoCard Reader.

The group A patients having diabetes with periodontitis showed nonsignificant increase in serum IgM level as compared to controls and other groups. Group B showed significance of p = 0.074. Group C showed significance of p = 0.982 and group D showed significance of p = 0.520. There was significant increase in HbA1c with an increase percentage serum IgM.

In the present study, significantly high concentrations of the IgM in serum of diabetic and nondiabetic patients were found as compared to the healthy subjects who had neither diabetes nor periodontitis.

By this study, we can emphasize on the fact of the importance of the immune system and its correlation with glycemic control, especially in patients diagnosed with periodontitis, also suggests that the alteration in immune response in poorly controlled diabetic patients may contribute to the pathogenesis of periodontitis and is the cause of increased incidence of periodontitis in patients with diabetes.

Kranthi J, Sudhakar KNV, Kulshrestha R, Raju PVK, Razdan A, Srinivasa TS. Comparison of the Serum Immunoglobulin IgM Level in Diabetic and Nondiabetic Patients with Chronic Periodontitis. J Contemp Dent Pract 2013;14(5):814-818.

Cardiac rehabilitation is a key part in the treatment of coronary artery disease (CAD) by its anti-inflammatory effects. However, the effect of exercise training programs on salivary concentrations of high-sensitivity C-reactive protein (hs-CRP) in patients with coronary artery disease has not been well studied. The objective of this study was to evaluate the effect of phase III cardiac rehabilitation on serum and salivary levels of hs-CRP, in relation to the anthropometric measurements of obesity and the relationship between salivary and serum levels of hs-CRP in CAD male patients.

Forty male volunteers (45-75 years) with CAD participated in 6 to 8 weeks of moderate intensity aerobic exercise training consisting of 45 minutes sessions of treadmill, stationary bicycle and arm ergometer. Anthropometric measurements of obesity, serum level of hs-CRP, stimulated and nonstimulated salivary level of hs-CRP were measured at the beginning, in the middle and at the end of exercise sessions.

All anthropometric measurements increased (p < 0.05) following cardiac rehabilitation except waist-hip ratio. Serum hs-CRP level reduced by 36% independent to the anthropometric measurements changes. Stimulated and nonstimulated salivary hs-CRP level decreased 68 and 54%, respectively, after 24 sessions of cardiac rehabilitation. Nonstimulated salivary hs-CRP levels correlated to serum levels of hs-CRP at baseline and after 24 sessions (p < 0.05).

Phase III cardiac rehabilitation seems to be effective to improve serum and salivary hs-CRP concentrations independent of anthropometric measurements.

Nonstimulated salivary hs-CRP measurement could be a surrogate for blood measurement of hs-CRP during cardiac rehabilitation in male patients with CAD.

Jamshidpour B, Moghadam BA, Vasaghi-Gharamaleki B, Mirzaii-Dizgah I, Nejatian M. The Effects of Phase III Cardiac Rehabilitation in Serum and Salivary Hs-CRP and Anthropometric Measurements in Patients with Coronary Artery Disease. J Contemp Dent Pract 2013;14(5):819-824.

The aim of this study was to compare the effectiveness of the F-file with an ultrasonically activated #15 K-file in removing the smear layer after biomechanical instrumentation along with irrigation of Saline, NaOCl and with or without a flush of EDTA.

Sixty decoronated human premolar teeth with a single canal were instrumented with ProTaper using S1, S2 and F1 series to produce the smear layer and randomly divided into two groups. Group A used Ultrasonics and group B used F-file for activation of irrigants respectively. Each group was further divided in to three subgroups consisting of 10 teeth in each as I, II, III consisting of saline, NaOCl, NaOCl and EDTA as irrigants respectively. SEM micrographs were taken and amount of smear layer removal was analyzed by using Chi-square statistics tests.

Most effective smear layer removal was seen only when EDTA was used. There was no statistically significant difference between the groups A and B in removal of smear layer.

There was no increase in smear layer between use of F-file when compared with the Ultrasonically activated K-file.

The F-file although does not have a superior efficacy than the ultrasonics in removal of smear layer from root canals but when used along with EDTA, can be an effective alternative for the dentists who are unable to bear the initial setup cost of ultrasonics.

Shenvi S, Kumar BS. An in vitro Study to Compare the Effectiveness of F-file with Ultrasonically Activated K-file to Remove Smear Layer by using a Scanning Electron Microscope. J Contemp Dent Pract 2013;14(5):825-829.

To study the effect of three energy drinks on the surface roughness of nanofilled composite resins after different periods of aging time.

Composite resin disks, 6 mm diameter, 3 mm thickness were prepared from Filtec Z350 XT, Tetric EvoCeram and Filtec Z250 XT. Specimens fr/8om each material were tested after aging with Red Bull, Bison and Power Horse energy drinks and distilled water as a control. Specimens were stored at 37°C in dark containers for 1, 3 and 6 months. Surface roughness Ra was assessed using a surface scanning interferometry before and after each storage period. Surface roughness differences ΔRa and Ra among specimens were measured. Mean values were statistically analyzed using multiple repeated measured (ANOVA), variance and multiple comparisons of the mean values were done with Bonferroni test, with p < 0.05 as significance level.

The surface roughness degradation change ?Ra and Ra value before and after occurred by Red Bull, Bison and Power Horse energy drinks was significantly different for all tested materials at all three times p < 0.05. All specimens showed increase in surface roughness changes after aging periods of 1, 3 and 6 months. The highest total surface roughness difference ΔRa and Ra was found in the Red Bull group after 6 months.

Energy drinks used in this study had surface degradation effect on the tested composite resin materials. The surface roughness increased with aging time however, it was clinically acceptable in all test groups after 6 months. The effect of energy drinks solutions on the surface roughness parameter of resin composites depends on type of solution and its acidity contents.

Al-Samadani KH. Effect of Energy Drinks on the Surface Texture of Nanofilled Composite Resin. J Contemp Dent Pract 2013;14(5):830-835.

Kumar TK, Thomas V, Nilawar S, Balamurugan R, Marwaha BS, Vinod V. A Cephalometric Study to Determine the Center of Anteroposterior Curve of Occlusion in the Cranium. J Contemp Dent Pract 2013;14(5):836-841.

This study was conducted to evaluate the antimicrobial activity of 5.25% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX) and BioPure MTAD when used as a final rinse against Enterococcus faecalis.

Sixty single-rooted premolars were biomechanically prepared, inoculated with E. faecalis and divided into various groups. These were then irrigated with the test irrigants and tested microbiologically for growth of E. faecalis immediately after irrigation and after 48 hours.

Statistical analysis showed that there was a significant difference between the antibacterial activities of BioPure MTAD, 2% CHX and 5.25% NaOCl at 5 minutes; however, the antibacterial activities of the three irrigants were comparable after 2 days of irrigation

The present study concludes that BioPure MTAD is as effective against E. faecalis as 5.25% NaOCl and more effective than 2% CHX.

E. faecalis is one of the most resistant intracanal species and a possible cause of root canal failure. Many authors have stressed the importance of using antimicrobial irrigants during chemomechanical preparation to ensure complete disinfection. Therefore, various irrigating solutions have been used during and immediately after root canal preparation to remove debris and necrotic pulp tissue and to eliminate microorganisms that cannot be reached by mechanical instrumentation.

Agrawal V, Rao MSR, Dhingra K, Gopal VR, Mohapatra A, Mohapatra A. An in vitro comparison of Antimicrobial Efficacy of Three Root Canal Irrigants—BioPure MTAD, 2% Chlorhexidine Gluconate and 5.25% Sodium Hypochlorite as a Final Rinse against E. faecalis. J Contemp Dent Pract 2013;14(5):842-847.

To evaluate and compare the efficacy of preprocedural mouthrinses (chlorhexidine digluconate and tea tree oil) in reducing microbial content of aerosol product during ultrasonic scaling procedures by viable bacterial count.

It was a randomized single blind, placebocontrolled parallel group study.

Sixty subjects were randomly assigned to rinse 10 ml of any one of the mouthrinses (chlorhexidine digluconate or tea tree oil or distilled water). Ultrasonic scaling was done for a period of 10 minutes in presence of trypticase soy agar plates placed at standardized distance. Plates were then sent for microbiological evaluation for the aerosol produced.

This study showed that all the antiseptic mouthwashes significantly reduced the bacterial colony forming units (CFUs) in aerosol samples. Chlorhexidine rinses were found to be superior to tea tree when used preprocedurally in reducing aerolized bacteria.

This study advocates preprocedural dural rinsing with an effective antimicrobial mouthrinse during any dental treatment which generates aerosols, reduces the risk of crosscontamination with infectious agents in the dental operatory.

The aerolization of oral microbes occurring during dental procedures can potentially result in cross-contamination in the dental operatory and transmission of infectious agents to both dental professionals and patient. It is reasonable to assume therefore, that any stratagem for reducing the viable bacterial content of these aerosols could lower the risk of cross-contamination.

Shetty SK, Sharath K, Shenoy S, Sreekumar C, Shetty RN, Biju T. Compare the Efficacy of Two Commercially Available Mouthrinses in reducing Viable Bacterial Count in Dental Aerosol produced during Ultrasonic Scaling when used as a Preprocedural Rinse. J Contemp Dent Pract 2013;14(5):848-851.

The aim of this study is to investigate the blood hemogram and estimation of serum iron and proteins level in aggressive periodontitis patients.

A total of 85 patients were selected and divided into two groups, 45 patients are with aggressive periodontitis and 40 patients are healthy. Periodontal parameters such as gingival inflammation oral hygiene index, Russell's periodontal index and radiograph were taken. Total 10 ml blood was collected and sent for estimation of blood hemogram protein estimation and serum electrophoresis.

There was statistical significant difference observed in relation to gingival inflammation oral hygiene hemoglobin and total iron binding capacity level between both the groups.

It has been concluded that periodontitis does not induce anemia like state, as the hematological and biochemical parameters were almost equally affected in periodontally healthy and periodontally diseased individuals, but some parameters showed statistical significant difference between the both groups.

In the present study, the clinical periodontal parameters, red blood cell parameters and serum iron and ferritin levels were compared among control and test groups. It was found that the values of gingival inflammation, oral hygiene and periodontal index, hemoglobulin level and total iron binding protein were statistically significant between the groups. However, the values of erythrocyte count, white blood cell count, serum iron and serum proteins, serum electrophoresis did not show any significant correlation.

Rao PK, Reddy RV, Mapare SA, Nag VR, Gowtham K, Arora D. An Investigation of Blood Hemogram and Estimation of Serum Iron and Protein Levels in Aggressive Periodontitis Patients: A Clinic Biochemical Study. J Contemp Dent Pract 2013;14(5):852-857.

This study consisted of 30 patients requiring either full coverage restoration or 3 unit fixed partial denture. Total of 40 restorations (n = 40) were made and were randomly assigned into four groups comprising 10 restoration (n = 10) in each group. Group C control where no desensitizer application was done, group BB applied with BisBlock dentin desensitizer (Bisco Inc.), group ST applied with Systemp desensitizer (Ivoclar Vivadent), group GC applied with GC Tooth Mousse desensitizer (GC Asia). Desensitizer application was done immediately after the tooth preparation. Sensitivity of the tested abutment was determined by the patient response to cold, hot and bite stimuli and were recorded on visual analog scale (VAS). Sensitivity level scores was evaluated at 4 time intervals, i.e. 1 week after desensitizer application at baseline precementation appointment and others at 5 minutes, 1 day and 1 week postcementation appointment. VAS score data was statistically analyzed using one-way ANOVA followed by post hoc Tukey's test.

BisBlock and GC Tooth Mousse desensitizer resulted in statistically significant (p < 0.01) reduction in postcementation sensitivity of glass ionomer cement in comparison to Systemp desensitizer at 5 minutes, 1 week postcementation time interval with no statistical difference was seen between all desensitizer groups at 1 day postcementation. Application of BisBlock and GC Tooth Mousse desensitizer resulted in highly significant (p < 0.01) reduction in sensitivity level at the end of 1 week.

Desensitizer's application on the prepared abutment teeth is considerably effective in relieving both pre- and postcementation sensitivity for full coverage restoration over the short duration of time. Immediate reduction in postoperative sensitivity relatively in a short time period may be beneficial in terms of patient's comfort. Nonetheless, multicenter long-term clinical trials should be conducted to confirm the results.

Efficacy of BisBlock and GC Tooth Mousse desensitizer was more in relieving the postcementation sensitivity of glass ionomer cement at various time intervals in comparison to Systemp desensitizer. In conclusion, application of desensitizers was beneficial to reduce the pre- and postcementation abutment sensitivity.

Gupta N, Reddy UN, Vasundhar PL, Ramarao KS, Varma KVVP, Vinod V. Effectiveness of Desensitizing Agents in relieving the Pre- and Postcementation Sensitivity for Full Coverage Restorations: A Clinical Evaluation. J Contemp Dent Pract 2013;14(5):858-865.

To assess the effect of different bracket base conditioning method on shear bond strength (SBS) of rebonded brackets.

Eighty brackets were bonded to freshly extracted premolar teeth using light cured composite adhesive. SBS was measured for 20 random samples as control group (G1). After debonding, 60 debonded brackets were allocated randomly into three groups of bracket base conditioning methods to remove the remaining adhesives. G2: bracket base cleaned with slow speed round carbide bur (CB), G3: cleaned with ultrasonic scaler (US), G4: cleaned with sandblasting (SB). After that, brackets were rebonded in the same manner as first bonding and SBS was measured. Modified adhesive remnant index (ARI) was recorded for all groups.

SBS for new brackets was 11.95 MPa followed by 11.65 MPa for G2, 11.56 MPa for G4 and 11.04 MPa for G3 group. There were no statistically significant differences between all groups (p = 0.946). In all groups, failure mode showed that the majority of adhesive composite remained on the bracket base with ARI of 4. There was no statistically significant difference between all groups in ARI (p = 0.584).

In-office methods; slow speed CB and US are effective, quick and cheap methods for bracket base cleaning for rebonding.

Al Maaitah EF, Alomari S, Alhaija ESA, Safi AAM. The Effect of Different Bracket Base Cleaning Method on Shear Bond Strength of Rebonded Brackets. J Contemp Dent Pract 2013;14(5):866-870.

The goal of this study was to find a method to eliminate bond strength reduction after vital bleaching.

Sixty flat surfaces of enamel central human incisors were prepared by diamond disks. In the first group, the samples immediately bonded to the tooth after bleaching. For the second group, the bleached samples were treated by sodium ascorbate for 1 hours. In the third and fourth groups the treatment was down using a new antioxidant agent for 1 hour and 15 minutes respectively. For the fifth group no bleaching was done. Cylindrical composite resin (Z 100) with a Single Bond adhesive system was bonded to the enamel with a special metal mold. After thermocycling, the samples were loaded at a crosshead speed of 0.5 mm/min. The mode of failures was inspected by the Dino Lite microscope and the microstructure of the debonding surfaces was observed by SEM. Considering normal data, statistical analysis was conducted by ANOVA and Tukey tests (p = 0.05).

The fourth group had maximum (21.82), while the first group had the least (9.0971) shear bond strength. The ANOVA test showed a significant difference among the five groups (p = 0.0002). There was not any significant statistical difference between the three types of debonding (p = 0.165).

The usage of sodium ascorbate or sodium ascorbate with detergent after vital bleaching are effective methods for reducing the damaging effects of bleaching agents on the shear bond strength of composite resin restoration to enamel.

Using antioxidants is necessary post bleaching and before resin bonding.

Moosavi H, Maleknejad F, Hoseinipour Z, Hatami L, Zeynali M. Antioxidant Agents and Their Effects on Shear Bond Strength of Bleached Enamel. J Contemp Dent Pract 2013;14(5):871-875.

This study aims to evaluate the degree of conversion (DC) and hydrolytic degradation through the Vickers hardness test (HV) of a nanofilled (FiltekTM Z-250, 3M) and a microhybrid (FiltekTMSupreme-XT, 3M) composite resin.

Eight disk-shaped specimens (4 mm diameter × 2 mm thick, ISO 4049) of each material were prepared for each test. Composites were inserted into single increment in a metallic matrix and light-cured for 40 seconds. VH readings were performed for each specimen at predetermined intervals: immediately after polymerization (control), 1, 2, 3, 7, 14, 21, 30 and 180 days. After curing, initial hardness measurements were performed and the specimens were immersed in artificial saliva at 37°C. For DC (%), specimens were ground, pressed with KBr and analyzed by FT-IR spectrophotometer.

Student t-test showed that there was no difference between the resins for DC (p = 0.252). ANOVA analysis revealed that Z-250 VH means were all greater than S-XT, for both top and bottom surfaces, whatever the storage-period in artificial saliva (p < 0.001). After 180 days of storage, the hardness obtained for S-XT was similar with that at the baseline, for both top and bottom surfaces. While for Z-250 hardness was not significantly different from baseline only for top surface, but there was a significant decrease observed in hardness for bottom surface.

The materials tested showed no evidence of hydrolytic degradation in a significant way, in a 6-month storagetime in artificial saliva. Nanofilled resin presents a monomer conversion comparable to the conventional microhybrid.

Jassé FF, Borges CHB, Tonetto MR, de Souza Rastelli AN, Bagnato VS, de Campos EA, Bandeca MC, Saad JRC. Long-Term Surface Hardness and Monomer Conversion of a Nanofilled and a Microhybrid Composite Resin. J Contemp Dent Pract 2013;14(5):876-882.

To determine the clinical applicability of Ormocer based flowable adhesive (Admira flow) in comparison with BisGMA based adhesive (Transbond XT) and Ormocer based packable adhesive (Admira).

Sixty human premolars, divided into group I (n = 20) Transbond XT, group II (n = 20) Admira and group III (n = 20) Admira flow were bonded with metal brackets using adhesives. Brackets were debonded in shear on an Instron universal testing machine with a crosshead speed of 1 mm per minute. The mode of bond failure was determined by modified ARI index.

The results obtained from SBS evaluation and modified ARI showed highest shear bond strength for Transbond XT (SD 11.64) 3.68 followed by Admira flow (SD 11.0) 2.87 and least for Admira (SD 9.42) 2.21. However, the difference was not statistically significant, but an intergroup comparison done using Independent student ‘t’ test, showed statically significant difference between Transbond XT and Admira. Kaplan-Meier survival analysis showed least survival median value for Admira, but the survival median value is not statistically significant among the three groups. All groups had modified ARI score of three (60-70%), suggestive of cohesive type of failure.

The in vitro study showed that flowable Ormocer can be an good alternative to commonly used BisGMA based adhesive but the its efficacy needs clinical assessment through a survival analysis.

Admire flow can definitely be considered as an alternative bonding system due to their comparable bond strength and debonding characters and reported properties of biocompatibility.

Kumar KS, Rao CH, Reddy KVB, Chidambaram S, Girish HC, Murgod S. Flowable Composite an Alternative Orthodontic Bonding Adhesive: An in vitro Study. J Contemp Dent Pract 2013;14(5):883-886.

The purpose of this study was to evaluate and compare in vitro the fluoride release and uptake from 5 different esthetic restorative materials.

Materials tested were conventional GIC — Fuji 7, GC (group1), resin modified GIC — Vitremer, 3M ESPE (group 2), polyacid modified composite — Dyract, Dentsply (group 3), fluoride releasing composite — Tetric ceram, Ivoclar Vivadent (group 4), Giomer-Beautifil, Shofu (group 5). Fluoride release was estimated at 6th, 24th, 48th hour and weekly interval for 5 weeks. For fluoride recharge, each specimen was exposed to 2 ml of 1.23% APF gel for 4 minutes and fluoride release measured at 1st, 2nd, 3rd day and 7th day. The fluoride release and recharge was measured using fluoride ion specific electrode.

ANOVA and Tukey HSD test were used for statistical analysis. During tested period all materials showed statistically different capability to release and uptake fluoride. Groups 1 and 2 results were comparable in fluoride release. Similarly groups 3 and 5. Groups 1 and 2 vs 3, 4, 5 were statistically very highly significant. Only conventional GIC showed initial ‘burst effect’.

Materials tested showed potential for fluoride release and recharge. Different esthetic materials had variable fluoride release hence selection of materials should be guided by specific clinical situations.

Gururaj M, Shetty R, Nayak M, Shetty S, Kumar CNV. Fluoride releasing and Uptake Capacities of Esthetic Restorations. J Contemp Dent Pract 2013;14(5): 887-891.

Acrylic resin ruled the dental profession for 60 years, and this success is attributed to its aesthetics, handling properties, physical and biological compatibility, its stability in oral environment and its cost effectiveness. The objective of this study is to evaluate and compare the bond strength of acrylic resin teeth treated with various conditioning materials like monomer and silane coupling agent.

A study was carried out in which 96 samples were grouped into 3 groups with a sample size of 32 each (16 premolars, 16 molars). They were conditioned with different conditioning materials i,e monomer and silane coupling agent. Monomer, Silane coupling agent are coated on the ridge lap area before thermocycling and cured according to the manufacturer recommendations. The samples are retained from the flask; trimmed and polished. The samples are then subjected to shear bond strength using the Insteron Universal Testing Machine.

In the present study it was found that application of monomer increased the bond strength between acrylic teeth and denture base, when compared to the conventionally processed samples. However it was found that application of silane coupling agent further increased the shear bond strength between acrylic teeth and denture base.

Within the confines of this study it is found that there was a significant improvement in the bond strength between the acrylic teeth and denture base when silane coupling agent and monomer were used as surface conditioning material. The order of shear strength of samples is control > monomer > silane coupling agent.

Madhav GV, Raj S, Yadav N, Mudgal I, Mehta N, Tatwadiya R. Shear Bond Strength of Acrylic Teeth to Acrylic Denture Base after Different Surface Conditioning Methods. J Contemp Dent Pract 2013;14(5):892-897.

Sadar L, Dhume S, Maniar N, Patil JP, Rane P, Gandhewar M. Comparative Evaluation of Shear Compressive Bond Strength between Cross-linked Acrylic Resin Denture Base and Cross-linked Acrylic Resin Teeth with Different Modifications of Their Ridge Lap Surfaces. J Contemp Dent Pract 2013;14(5):898-903.

To evaluate the oral mucosal status in the elderly population of different age group and find out the association of age, gender and denture with oral mucosal disorders.

The study sample consisted of 570 geriatric persons concentrating mainly on the oral mucosal changes or lesions occurring in the geriatric population. Individuals those are aged above 60 years were selected, and all the examined geriatric persons were categorized into 3 age groups to find out the association of oral mucosal lesions in each group.

Group I—60 to 65 years, Group II—66 to 70 years, Group III—71 and above years.

The sample of 570 elderly patients included 279 (48.95%) men and 291 (51.05%) women in three age groups: 61 to 65 years (40.35%), 66 to 70 years (31.05%), and 71 years and older (28.60%). The sample included 254 (44.56%) dentate patients, 205 (35.96%) denture wearers (partial and complete denture wearers) and 111 (19.47%) edentulous persons who lacked dentures in both the jaws. Almost half of the patients examined (48%) had one or more oral mucosal lesions. The 48% of the patients who presented with oral mucosal lesions, twenty five different oral mucosal conditions were identified and the three most common findings were lingual varices (13.68%), denture induced inflammatory fibrous hyperplasia (4.21%), squamous cell carcinoma (4.21%). There was some differences in the distribution of oral mucosal condition among the sexes. Leukoplakia and dysplasia were significantly associated with men (p < 0.001) whereas the association of fibroma and lichen planus with women were significant (p < 0.001).

In our study it was found that patients in groups II and III had more prevalence of oral mucosal disorders. Lingual varices, oral squamous cell carcinoma, fibroma and denture induced inflammatory fibrous hyperplasia were more commonly associated with the geriatric patients. The oral lesions (fibroma and lichen planus) were strongly associated with women while leukoplakia was strongly associated with men. Ageing is an important factor that can influence the occurrence of mucosal lesions and with age the oral mucosa becomes more permeable to noxious substances and more vulnerable to external carcinogens.

Shet RGK, Shetty SR, M Kalavathi, Kumar MN, Yadav RD, S Soumya. A Study to evaluate the Frequency and Association of Various Mucosal Conditions among Geriatric Patients. J Contemp Dent Pract 2013;14(5):904-910.

It is now understood and accepted that there is a direct transmission of mutans streptococci (MS) from the mother to the child. There is also a direct correlation between the levels of MS in the mother and the caries status of the child. Advanced technologies in molecular biology like chromosomal DNA fingerprinting have established beyond doubt that the mother and the child bear similar strains of MS.

A study was designed with the aim of comparing the MS strains between the father, mother and the child in Indian families.

A group of 20 Indian families comprising of the father, mother and child were selected and divided into caries free and caries active groups. Mixed salivary samples were collected from the individuals and were cultured for the growth of Mutans streptococci. The colonies were counted on a colony counter and a comparison was made between the mutans streptococcal counts of the mother and the caries status of the child. Further, the genotypes of the father, mother and the child were isolated and compared using the technique of chromosomal DNA fingerprinting. Following electrophoresis, the band pattern obtained was compared for similarities or differences. The results of the same were tabulated and evaluated statistically

When the colony counts of the mother (in CFU/ml) were compared with the ‘dft’ status of the child, a positive correlation was seen in group II. Intergroup comparison using the unpaired T test was statistically significant. Electrophoretic analysis of the chromosomal DNA on the agarose gels revealed identical band patterns in 13 mother-child pairs, which was statistically significant. Three of the father-child pairs showed identical band patterns, which was statistically significant. Intergroup comparison using Chi-square test was not statistically significant.

One may conclude that irrespective of the caries status of the child, majority of the mother child pairs share identical strains of MS and hence the mother is the primary source of infection. However, in children with a high dft, the father may also play an important role in the acquisition and transmission of MS.

Katre AN, Damle SG. Comparison of Mutans Streptococcal Strains of Father, Mother, and Child in Indian Families using Chromosomal DNA Fingerprinting. J Contemp Dent Pract 2013;14(5):911-916.

The aim of this study was to determine and compare the shear bond strength of three different pits and fissure sealants to enamel namely, Delton, Clinpro and Z350 Filtek flowable composite.

Ninety permanent molars were divided into three groups of 30 each. Their proximal surfaces were prepared and stored in 37°C ± 5°C normal saline for 24 hours. Sealant buttons were prepared. The specimens were tested in a shear mode (Lloyds LR100K) at a crosshead speed of 0.5 mm/min. The mean and standard deviations were subjected to an analysis of variance.

The mean shear bond strength of samples of Delton was 10.33 MPa; Clinpro was 8.51 MPa and Z350 Filtek flowable was 12.17 MPa. The mean strength of Delton was significantly higher as compared to Clinpro whereas the mean strength of Z350 Filtek was significantly higher as compared to Delton and Clinpro (Student t-test, p < 0.001).

Z350 Filtek flowable composite had maximum shear bond strength than Delton FS sealant followed by Clinpro sealant.

In this study, we compare the bond strength of three pits and fissure sealant, Clinpro yielded lower bond strength than Delton. This outcome can be described due to the presence of fillers in Delton, since these particles increase the resistance of the material to abrasion and also increase the mechanical resistance of the material.

Singh N, Agrawal G, Subhash AV, Suneela S, Barabde AS, Kumar GA. A Comparative Evaluation of Shear Bond Strength of Different Pits and Fissure Sealants: An in vitro Study. J Contemp Dent Pract 2013;14(5):917-923.

The present study was conducted to evaluate the efficacy of commercially available herbal toothpastes against the different periodontopathogens.

Six herbal toothpastes that were commonly commercially available were included in the study. Colgate herbal, Babool, Meswak, Neem active, Dabur red toothpastes were tested for the study whereas sterile normal saline was used as control. Antimicrobial efficacies of dentifrices were evaluated against Streptococcus mutans and Actinobacillus actinomycetemcomitans. The antimicrobial properties of dentifrices were tested by measuring the maximum zone of inhibition at 24 hours on the Mueller Hinton Agar media inoculated with microbial strain using disk diffusion method. Each dentifrice was tested at 100% concentration (full strength).

The study showed that all dentifrices selected for the study were effective against the entire test organism but to varying degree. Neem active tooth paste gave a reading of 25.4 mm as the zone of inhibition which was highest amongst all of the test dentifrices. Colgate Herbal and Meswak dentifrices recorded a larger maximum zone of inhibition, measuring 23 and 22.6 mm respectively, compared to other toothpastes. All other dentifrices showed the zone of inhibition to be between 17 and 19 mm respectively.

The antibacterial properties of six dentifrices were studied in vitro and concluded that almost all of the dentifrices available commercially had antibacterial properties to some extent to benefit dental health or antiplaque action.

Jenner F, Jaleel VA, Kulshrestha R, Maheswar G, Rao PK, Kranthi J. Evaluating the Antimicrobial Activity of Commercially Available Herbal Toothpastes on Microorganisms Associated with Diabetes Mellitus. J Contemp Dent Pract 2013;14(5):924-929.

The aim of this study is to investigate the effect various dietary nutrients in aggressive periodontitis patients.

A total of 85 patients were selected and divided into two groups, 45 patients are with aggressive periodontitis and 40 patients are healthy. Periodontal parameters such as oral hygiene index, Russels periodontal index and radiograph were taken. The food consumption survey was conducted in all the households of subjects both in control and experimental group. The individual of food intake of the subjects was assessed by the oral questionnaire (24 hours recall) method. The quantity of raw foods used for various preparations and volumes of cooked quantities of such preparations in terms of standardized cups were noted. Body measurements were taken on all the subjects, standing height using a height measuring rod and weight in standard weighing machine.

Aggressive periodontitis is seen in young individual and mostly in females; majority of the patients of both groups belongs to low socioeconomic group. Body mass index which is a reflection of nutritional status of an individual indicated that chronically energy deficient subjects in experimental group appeared to be higher. The average food and nutrient intake in control group was slightly better than that of experimental group.

The diet survey indicated marginal and negligible deficiencies in aggressive periodontitis patients compared to controls, and this coupled with chronically energy deficiency as indicated by body mass index, calls for a detailed study of this aspect of aggressive periodontitis. The present study indicates that nutritional influences point to a needle of suspicion toward the etiology of aggressive periodontitis.

Mapare SA, Rao PK, Reddy RV, Kumar MGM, Gorthi VSSC, Raju PVK. A Comparative Clinical Evaluation of Diet Intake and Effect of Various Nutritions on Aggressive Periodontitis Patients. J Contemp Dent Pract 2013;14(5):930-938.

Myofascial pain dysfunction syndrome (MPDS) is a disorder which is characterized by facial pain and limited mandibular function because of the complex nature of this disease. Malocclusion was never evaluated in MPDS patients to know its role. Electromyographic silent period durations of masseter were used for objective assessment of MPDS patients.

To evaluation of role of malocclusion in MPDS patients and also to study the EMG masseteric silent period pattern in MPDS patients in different malocclusions.

Two groups, groups I and II of 75 subjects each, were included in this study. Groups I and II constituted the normal subjects and MPDS patients respectively. All these subjects in both the groups were again subdivided based on Angle's class I, II and III malocclusion. Electromyographic (EMG) silent period of masseteric muscle on both sides was measured for all the subjects in both the groups to know whether it differs according to the type of malocclusion.

The silent period was more in group II (MPDS patients). There was no significant difference in the silent periods in Angle's class I, II and III malocclusion in group I, whereas in group II, there was a significant difference in the silent period in Angle's class II compared to Angle's class I and III. There was no significant difference between males and females.

MPDS patients are more in Angle's class I malocclusion. Silent period is more in Angle's class II malocclusion of MPDS group. Clinical significance: The EMG masseter silent period duration can be advantageously utilized as an adjunct to clinical examination for diagnosis of myofascial pain dysfunction syndrome. It is enough if one side masseter muscle is measured for silent period duration.

Varma KVVP, Reddy RB, Reddy SNK, Rao SR, Neela PK, V Naresh. Evaluation of Malocclusion in MPDS. J Contemp Dent Pract 2013;14(5):939-943.

Jindal G, Kumar D. Developmental Malformation of Primary and Permanent Dentition: A Rare Sequel of Trauma. J Contemp Dent Pract 2013;14(5):944-947.

Current treatment of gingival recession (GR) is limited to surgical procedures. We describe a case series of 18 patients with GR who were treated with a botanical patch and rinse following standard conservative therapy.

A total of 22 sites with GR > 1 mm were studied. Following scaling and root planing (SRP) and oral hygiene instruction, patients received two courses of patch treatment (3 days each) and botanical rinse administered twice daily throughout the treatment period. Outcome measures (GR, gingival index (GI) and gingival thickness (GT) were taken at baseline; at 1 to 2 weeks; 2 to 4 weeks; and at 6 to 8 weeks. Miller classification and plaque index (PI) were measured at baseline and at 6 to 8 weeks. At the end of the treatment period, mean GR decreased from 4.18 ± 1.74 mm to 3.31 ± 1.51 mm (20.8%); Miller grade from 1.86 ± 0.56 to 1.06 ± 0.43; GI scores from 1.45 ± 0.63 to 0.17 ± 0.38 (88.3%); and PI scores from 1.33 ± 0.59 to 0.78 ± 0.94. GT increased from 0.74 mm ± 0.40 to 1.21 ± 0.39 (63.5%). No adverse effects were reported with either the patch or rinse treatments

We observed a decrease in GR and GI scores in 18 patients (22 sites) treated with the study patch and rinse, with increased GT.

Levine WZ, Samuels N, Sheshet MEB, Grbic JT. A Novel Treatment of Gingival Recession using a Botanical Topical Gingival Patch and Mouthrinse. J Contemp Dent Pract 2013;14(5):948-953.

The use of headgear can lead to the rare complication of alopecia and the clinician should be aware of it.

The reader should understand the psychological implications of alopecia and also to carry out the treatment after assessing the risk/benefit ratio.

Premkumar S, Vidya K. Pressureinduced Alopecia from Orthodontic Headgear. J Contemp Dent Pract 2013;14(5):954-956.

The purpose of this case report is to present the orthodontic, surgical and restorative treatments in the case of an operated cleft lip and palate and severe maxillary deficiency in a 14-year-old female patient.

Only orthodontic treatment could be inefficient for cleft lip and palate patients characterized with maxillary hypoplasia. Orthodontic and surgical treatment shows sufficient results, especially with severe skeletal deficiency.

A cleft lip and palate patient required complex multidisciplinary treatment to preserve health and restore esthetics. Dental leveling and alignment of the maxillary and mandibular teeth were provided before the surgery. Maxillary advancement and clockwise rotation of the maxillary-mandibular complex was applied by a Le Fort 1 osteotomy with two internal distraction devices. After the active treatment including orthodontic treatment and orthognathic surgery, upper full mouth ceramic restoration was applied.

This report shows the efficiency of internal distraction devices in cleft lip palate patients and exemplifies the multidisciplinary care required for such difficult cases.

Stable improved occlusion and skeletal relations were observed after a follow-up examination period of 12 months.

Öz AA, Özer M, Eroglu L, Özdemir OS. The Correction of Maxillary Deficiency with Internal Distraction Devices: A Multidisciplinary Approach. J Contemp Dent Pract 2013;14(5):957-962.

Lele GS, Modi D. Impeded Eruption of mandibular Canine. J Contemp Dent Pract 2013;14(5):963-967.

Although, dental implants have been a predictable option to support several types of restorations, mechanical problems are not uncommon. Retaining and abutment screw loosenings/fractures are one of the most common technical problems.

To present a step-by-step technique in order to remove a broken locater abutment screw fragment.

A 56-year-old, edentulous man with a fractured locator abutment screw in an implant was referred to our clinic from a private practice. His dentist placed a narrow platform locator abutment onto a regular platform implant and the fracture occurred. Attempts to remove the fractured fragment using a periodontal probe and a manual screw removal driver were unsuccessful.

This clinical report presents a situation in which a fractured locater abutment screw fragment was successfully removed using an implant repair kit.

A fractured locater abutment screw fragment was successfully removed using an implant repair kit including drills, drill guides and tapping instruments. To avoid similar complications, it is recommended to use appropriate manufacturer specified torque values in conjunction with compatible restorative components.

Retaining and abutment screw loosenings/fractures are one of the most common mechanical problems associated with the implant components. This clinical report showed how to remove a broken screw fragment using proper armamentarium and technique, which might help clinicians eliminate similar problems.

Turkyilmaz I, Vierra MJ, Asar NV. Removal of a Fractured Locater Abutment Screw Fragment. J Contemp Dent Pract 2013;14(5):968-972.

Patel N, Patel K, Venkataraghavan K, Madan S. Utilization of Different Management Concepts in Fractured Tooth Fragment Reattachment: A Report of Three Cases. J Contemp Dent Pract 2013;14(5):973-979.

Mohammadi Z, Shalavi S, Jafarzadeh H. Extra Roots and Root Canals in Premolar and Molar Teeth: Review of an Endodontic Challenge J Contemp Dent Pract 2013;14(5):980-986.

Patil S, Rao RS, Ganavi BS. The Museum Maze in Oral Pathology Demystified: Part II. J Contemp Dent Pract 2013;14(5):987-992.

Jasoria G, Shamim W, Rathore S, Kalra A, Manchanda M, Jaggi N. Miniscrew Implants as Temporary Anchorage Devices in Orthodontics: A Comprehensive Review. J Contemp Dent Pract 2013;14(5):993-999.

Ahuja T, Jaggi N, Kalra A, Bansal K, Sharma SP. Hemangioma: Review of Literature. J Contemp Dent Pract 2013;14(5):1000-1007.