The Journal of Contemporary Dental Practice

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2024 | March | Volume 25 | Issue 3

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EDITORIAL

Seyed Ali Mosaddad

Arthroscopy for the Treatment of Temporomandibular Disorders

[Year:2024] [Month:March] [Volume:25] [Number:3] [Pages:2] [Pages No:197 - 198]

Keywords: Arthroscopy, Facial pain, Temporomandibular joint, Temporomandibular joint disorders

   DOI: 10.5005/jp-journals-10024-3646  |  Open Access |  How to cite  | 

372

ORIGINAL RESEARCH

Maria Saadeh, Loubna Shamseddine, Hasan Fayyad-Kazan, Fouad Ayoub

Nasal Morphology in a Young Adult Middle-Eastern Population: A Stereophotogrammetric Analysis

[Year:2024] [Month:March] [Volume:25] [Number:3] [Pages:8] [Pages No:199 - 206]

Keywords: 3D anthropometry, Lebanon, Nasal morphology, Stereophotogrammetry

   DOI: 10.5005/jp-journals-10024-3649  |  Open Access |  How to cite  | 

Abstract

Aim: This study aimed to describe gender-specific three-dimensional morphology of the soft-tissue nose in Lebanese young adults and to explore the associations between nasal morphology with age and body mass index (BMI). Materials and methods: Three-dimensional photographs were captured for 176 young healthy Lebanese adults (75 males and 101 females) aged 18.1–37.68 years. Linear and angular nasal measurements were computed and compared between genders, in addition to other established norms. Associations with age and BMI were also assessed. Results: All linear measurements were greater in males than in females, and only the nasolabial angle was significantly larger in females by 2.97 degrees on average. Most of the measurements were found to be larger than the Caucasian norms. A few significant correlations were found between the measurements and age or BMI. Conclusion: This study is the first to present the sex-specific norms for nasal morphology in the Lebanese population and highlights the presence of gender dimorphism in the majority of measurements. Additional studies are needed to validate our data and expand the associations with age and BMI. Clinical significance: The data offered in this study could help enhance the accuracy of facial reconstructive surgery and aid in personalized treatment planning for both medical and cosmetic nasal interventions.

322

ORIGINAL RESEARCH

Alice Helena de L Santos Cardoso, Marcelo Palinkas, Nicole B Bettiol, Patrícia S de Lima, Paulo B de Vasconcelos, Samuel P Xavier, Gabriella SG Silva, Thamyres Branco, Isabela H Regalo, Selma Siessere, Simone CH Regalo

Bichectomy Surgery and EMG Masticatory Muscles Function in Adult Women: A Longitudinal Study

[Year:2024] [Month:March] [Volume:25] [Number:3] [Pages:6] [Pages No:207 - 212]

Keywords: Bichectomy surgery, Buccal fat removal, Electromyography, Masticatory muscles, Stomatognathic system

   DOI: 10.5005/jp-journals-10024-3656  |  Open Access |  How to cite  | 

Abstract

Aim: This longitudinal study aimed to evaluate the electromyographic activity of the masseter and temporal muscles in adult women who underwent buccal fat removal. Materials and methods: The sample consisted of 20 healthy adult women with no temporomandibular dysfunction and normal occlusion, who were assessed before, 30, and 60 days after the surgery. The electromyographic signal of the masseter and temporal muscles was captured through mandibular tasks including rest, protrusion, right and left laterality, and maximum voluntary contraction with and without parafilm. The results obtained were tabulated and the Shapiro-Wilk normality test was performed, which indicated a normal distribution. Statistical analysis was performed using the repeated measures test (p < 0.05). Results: Significant differences were observed between time periods in maximum voluntary contraction for the left masseter muscle (p = 0.006) and in maximum voluntary contraction with parafilm for the right temporal (p = 0.03) and left temporal (p = 0.03) muscles. Conclusion: Bichectomy surgery did not modify the electromyographic activity of the masseter and temporal muscles during the rest task but may have influenced variations in the electromyographic signal during different mandibular tasks after 60 days of surgery, suggesting compensatory adaptations and functional recovery. Clinical significance: Understanding the impact of buccal fat removal surgery on the stomatognathic system function provides insights into postoperative functional recovery and potential compensatory adaptations, guiding clinical management and rehabilitation strategies for patients undergoing such procedures.

301

ORIGINAL RESEARCH

Antoine Berberi, Amine el Zoghbi, Georges Aad, Georges Tehini

Immediate Loading Using the Digitalized Customized Restoration of Single-tooth Implants Placed in Fresh Extraction Sockets in the Aesthetic Anterior Maxilla: A 10-Year Prospective Study of Marginal Bone Level

[Year:2024] [Month:March] [Volume:25] [Number:3] [Pages:8] [Pages No:213 - 220]

Keywords: Computer-assisted design/computer-assisted manufacturing systems, Immediate loading, Marginal bone level, Maxilla, Single implant

   DOI: 10.5005/jp-journals-10024-3659  |  Open Access |  How to cite  | 

Abstract

Aim: The objective of this study was to assess marginal bone level around single implants inserted in fresh extraction sockets in the anterior maxillary region and instantly restored with computer-aided design/computer-aided manufacturing customized temporary crowns cemented on the final abutment. Materials and methods: A total of 20 patients (15 females and 5 males, with a mean age of 30 years), where 20 were placed in fresh extraction sockets. After raising a full-thickness flap, atraumatic extraction was performed the implant site was prepared and fixtures were stabilized on the palatal bone wall. The implant location was immediately transmitted to the prepared master model using the pick-up impression coping seated in the surgical guide template. Prefabricated abutments were used as the final abutment on the master model, scanned and the crown was planned using computer-aided manufacturing customized software. Later on 8th weeks, abutments were torqued as per the manufacturer's recommendation, and the final crowns were cemented. Using personalized intraoral radiographs marginal bone level was evaluated mesially and distally to the implant shoulder as a reference at implant placement, 8 weeks, 1, 3, 5, and 10 years after loading. Results: Wholly implants were osteo-integrated positively after 10 years of practical loading, but only 18 were available for clinical and radiological follow-up, and 2 patients with two implants were excluded from the study due to relocation abroad without any implant failure. The average marginal bone loss (MBL) in the current report was 0.16 ± 0.167 mm at crown cementation, 0.275 ± 0.171 mm after 1 year, 0.265 ± 0.171 mm after 3 years, 0.213 ± 0.185 mm after 5 years, and 0.217 ± 0.194 mm at 10 years. Conclusion: The strategy of inserting and not removing the final abutment at the time of implant placement facilitates the establishment of adequate attachment of both soft and hard tissues to the abutment surface, ensuring uninterrupted organization of tissue architecture and offers advantages in helping maintain soft tissue maturation and preventing marginal bone level. Clinical significance: Immediately loaded implants in freshly extracted sockets lead to a significant reduction in marginal ridge resorption. The use of a temporary crown on a prefabricated abutment, exclusive of successive abutment manipulation, proved effective in preserving the primarily founding blood clot and served as a prototype for shaping the soft tissue around the previously wounded gum.

488

ORIGINAL RESEARCH

Esraa H Saber, Mohsen H Abielhassan, Yasser A Abed, Shereen E Fahim

Color Stability of Bioactive Restorative Material vs Nanohybrid Resin Composite: An In Vitro Study

[Year:2024] [Month:March] [Volume:25] [Number:3] [Pages:5] [Pages No:221 - 225]

Keywords: Color, Color change, Color stability, Composite, Nanohybrid composite resins, Resin composite

   DOI: 10.5005/jp-journals-10024-3654  |  Open Access |  How to cite  | 

Abstract

Aim: This study aimed to assess the color stability of bioactive restorative materials vs nanohybrid resin composites after 3 months of immersion in three frequently consumed beverages. Materials and methods: Thirty disk-shaped specimens of Giomer dental restorative material (Shofu, Japan) and nanohybrid resin composite (Tokuyama, Japan) were performed using a Teflon mold. Super-Snap system (Shofu, Japan) was utilized to finish and polish the specimens to be preserved for 24 hours in distilled water at 37°C. The samples had been divided into three subgroups (Coffee, tea, Pepsi) (n = 5). The initially displayed color measurements of the samples were performed using a spectrophotometer (VITA Easyshade® V). After 7 days, 30 days, and 90 days, color measurements were repeated, and the ΔE of each sample was estimated. ΔE of each sample was calculated. Results: The Giomer group showed statistically significant higher ΔE values than the nanohybrid resin composite where the p-value was ≤0.0001. Tea subgroup showed the highest statistically significant ΔE values in both groups where the p-value was ≤ 0.0001. The highest statistically significant color change was recorded at 3 months. Conclusion: The color of bioactive restorative material is less stable if compared with nanohybrid resin composite. Clinical significance: As tea and coffee are popular beverages, particularly in Middle Eastern nations, dentists must advise patients about the color change of resin restorations. Patients are advised to brush their teeth immediately after consuming these beverages.

397

ORIGINAL RESEARCH

Manasvi S Yenamandra, Asha Joseph, Prabath Singh, Ramanarayanan Venkitachalam, Remya Maya, Gayathri Presannakumar

Effect of Various Surface Treatments of Zirconia on its Adhesive Properties to Dentin: An In Vitro Study

[Year:2024] [Month:March] [Volume:25] [Number:3] [Pages:5] [Pages No:226 - 230]

Keywords: Bond strength, Resin cements, Sandblasting, Zirconia

   DOI: 10.5005/jp-journals-10024-3663  |  Open Access |  How to cite  | 

Abstract

Aim: To assess the effectiveness of various surface treatments and adhesives on the bond strength of zirconia-based ceramic to dentin. Materials and methods: Eighty samples of zirconia were subjected to the four-surface treatment protocols (sandblasting, 48% hydrofluoric acid (HF), 48% hydrofluoric acid + 70% nitric acid (HNO3) and no treatment (control) following which the samples from each group were subdivided into two subgroups (n = 10) based on the resin cement employed for cementation (RelyX U200 and G-Cem Linkforce). The bonded specimens were subjected to shear stress to measure the bond strength using Universal testing machine. To test the difference in bond strength among the eight study groups, the Kruskal–Wallis ANOVA test was applied and for comparison between cements in each group, Mann–Whitney U test was applied. Results: The highest bond strength values were observed for 48% HF group cemented with G-Cem Linkforce resin cement (16.220 ± 1.574) and lowest for control group–RelyX (4.954 ± 0.972). G-Cem cement showed higher bond strength than RelyX for all surface treatments except 48% HF + 70% nitric acid. Conclusion: It can be inferred that 48% HF can etch zirconia and generate a porous structure that proves to be beneficial for bonding. Clinical significance: The increasing demand for esthetics has led to the replacement of metal-ceramic materials with zirconia-based ceramics. However, the chemical inertness of zirconia to various conventional surface treating agents has continuously challenged researchers to discover a new surface treatment protocol that could enhance the bond strength of zirconia.

423

ORIGINAL RESEARCH

Alireza Adl, Nooshin S Shojaei, Nikta Ranjbar

The Effect of Adding Various Antibiotics on the Push-out Bond Strength of a Resin-based Sealer: An In Vitro Study

[Year:2024] [Month:March] [Volume:25] [Number:3] [Pages:5] [Pages No:231 - 235]

Keywords: AH Plus, Amoxicillin, Bond strength, Clindamycin, Triple antibiotic mixture

   DOI: 10.5005/jp-journals-10024-3660  |  Open Access |  How to cite  | 

Abstract

Aim: This study aimed to compare the bond strength of AH Plus sealer to root canal dentin when used with or without various antibiotics including amoxicillin, clindamycin, and triple antibiotic mixture (TAM). Materials and methods: A total of 80 single-rooted extracted human teeth were instrumented and obturated with gutta-percha and four different sealer-antibiotic combinations (n = 20). Group I: AH Plus without any antibiotics, Group II: AH Plus with amoxicillin, Group III: AH Plus with clindamycin, and Group IV: AH Plus with TAM. After seven days, the roots were sectioned perpendicular to their long axis and 1 mm thick slices were obtained from the midroots. The specimens were subjected to a push-out bond strength test and failure modes were also evaluated. Data was analyzed using Kruskal-Wallis and Dunn's post hoc tests. Results: Group IV had significantly higher bond strength compared to other groups (p ≤ 0.05). No significant differences were found between other groups. While the sealer-antibiotic groups predominantly showed cohesive failure modes, the control group displayed both cohesive and mixed failure modes. Conclusion: Within the limitations of this study, the addition of TAM increased the push-out bond strength of AH Plus. Clinical significance: Amoxicillin, clindamycin, or TAM can be added to AH Plus for increased antibacterial efficacy without concern about their effects on the bond strength of the sealer.

311

ORIGINAL RESEARCH

S Karthika, Sageena George, Anandaraj Soman, Shiana Jo, Fahanna Beegum, Mohammed A Habibullah

Salivary Proteinase 3 as a Biomarker for Caries Severity in Children: A Cross-sectional Study

[Year:2024] [Month:March] [Volume:25] [Number:3] [Pages:5] [Pages No:236 - 240]

Keywords: Biomarker, Caries severity, Dental caries, Proteinase 3

   DOI: 10.5005/jp-journals-10024-3648  |  Open Access |  How to cite  | 

Abstract

Aim: This study aims to evaluate the relation between salivary proteinase 3 (PR3) concentration and caries severity in children. Materials and methods: Six-to-eight-year age group children, from the Outpatient Department of Pediatric and Preventive dentistry at PMS Dental College were selected for the study. From these children, three groups each consisting of 28 children were selected according to the dental caries severity. Three groups were: (1) No Dental Caries group, (2) Low Dental Caries group with DMFT/DEFT score of 1–4, and (3) High Dental Caries group with DMFT/DEFT score of 5–15. Thus, a total of 84 children who satisfied the inclusion criteria were selected. The concentration of PR3 in saliva of the donors were analyzed using an ELISA kit. One way ANOVA was used for finding the relation of salivary PR3 concentration with caries severity. Pairwise comparison of PR3 concentration and caries severity were analyzed using post hoc Tukey test. Results: Severity of caries and concentration of salivary PR3 showed an inverse relation. As the caries severity increases there was a decrease in PR3 concentration and vice versa. Conclusion: The children with high caries severity showed lower concentration of PR3 in their saliva compared with those with lower caries severity which indicates that PR3 can be used as a biomarker for assessing caries severity and also paves way to use PR3 as a caries vaccine in future. Nowadays, interest toward noninvasive and personalized dentistry has been increased. Molecular assays using salivary biomarkers can be an effective tool in detecting the caries in earlier stages and assessing a patient's caries risk. Clinical significance: Salivary PR3 can be used as prognostic biomarker for assessing caries severity and after treatment the value of PR3 can be used as a assessment tool to confirm its relation with caries.

317

ORIGINAL RESEARCH

Karthik Kannaiyan, Aradhana Rathod, Purnendu Bhushan, M Shilpa, Turki Almuraikhi, Alhussain Daghriri

Assessment of Adaptability and Linear Dimensional Changes of Two Heat Cure Denture Base Resin with Different Cooling Techniques: An In Vitro Study

[Year:2024] [Month:March] [Volume:25] [Number:3] [Pages:4] [Pages No:241 - 244]

Keywords: Adaptability, Cooling techniques, Denture base materials, Dimensional change

   DOI: 10.5005/jp-journals-10024-3612  |  Open Access |  How to cite  | 

Abstract

Aim: The current study was designed to assess the linear dimensional changes and adaptability of two heat-cured denture base resins using various cooling methods. Materials and methods: To prepare a total of 90 acrylic resin samples (45 acrylic resin samples for each material), four rectangular stainless-steel plates measuring 25 × 25 × 10 mm were fabricated. For both groups, the material was put into the mold at the dough stage. Group I – SR Triplex Hot Heat Cure acrylic; group II – DPI Heat Cure acrylic. Both groups used the same curing procedure. One of the following three techniques was used to cool the material (15 samples from each material) once the curing cycle was finished: (A) water bath, (b) quenching, and (C) air. A traveling microscope was used to measure the distance between the markings on the acrylic samples. The data was recorded and statistically analyzed. Results: In SR Triplex Hot heat cure acrylic material, the maximum linear dimensional changes were found in the quenching technique (0.242 ± 0.05), followed by the air technique (0.168 ± 0.11) and the least was found in the water bath technique (0.146 ± 0.01). In DPI Heat Cure acrylic material, the maximum linear dimensional changes were found in the quenching technique (0.284 ± 0.09), followed by the air technique (0.172 ± 0.18) and the least was found in the water bath technique (0.158 ± 0.10). There was a statistically significant difference found between these three cooling techniques. On comparison of adaptability, the water bath technique, the marginal gap SR Triplex Hot was 0.012 ± 0.02 and DPI Heat Cure was 0.013 ± 0.02. In the quenching technique, the marginal gap SR Triplex Hot was 0.019 ± 0.04 and DPI Heat Cure was 0.016 ± 0.04. In the air technique, the marginal gap SR Triplex Hot was 0.017 ± 0.01 and DPI Heat Cure was 0.019 ± 0.01. Conclusion: The present study concluded that among the different cooling methods, the water bath technique had the least linear dimensional change, followed by the air and quenching techniques. When comparing the materials, DPI Heat Cure acrylic resin showed a greater linear dimensional change than SR Triplex Hot heat cure acrylic resin. Clinical significance: During polymerization, heat-cured acrylic resins experience dimensional changes. Shrinkage and expansion are dimensional changes that occur in heat-cured acrylic resins and have an impact on the occlusal relationship and denture fit. However, the denture base's material qualities and the different temperature variations it experiences during production may have an impact on this.

225

ORIGINAL RESEARCH

Navya Kuchibhotla, Hrishikesh Sathyamoorthy, Srinath Balakrishnan, Naga Praneeth Somaraju, Aakansha Mohan, Kishore Ginjupalli, N. Sridhar

Effect of Bonding Agents on the Shear Bond Strength of Tooth-colored Restorative Materials to Dentin: An In Vitro Study

[Year:2024] [Month:March] [Volume:25] [Number:3] [Pages:5] [Pages No:245 - 249]

Keywords: Bonding agent, Composite, Dentin bonding, Shear bond strength

   DOI: 10.5005/jp-journals-10024-3662  |  Open Access |  How to cite  | 

Abstract

Aim: The aim of the study is to determine the difference in the shear bond strengths to dentin among dental composite (Filtek Z350®, 3M), compomer (Dyract Flow®, Dentsply) and Giomer (Beautifil®, Shofu) with 3MTM Single BondTM Universal Adhesive (SBU) (7th generation, self-etch, single solution adhesive) and AdperTM Single Bond 2 Adhesive (ASB) (5th generation, total-etch, two solution adhesive). Materials and methods: Sixty extracted human permanent teeth were collected, cleansed of debris, and placed in distilled water. The samples were segregated into two groups depicting the two bonding agents—AdperTM (ASB) and 3MTM Single Bond Universal (SBU) and sub-grouped into three groups depicting the three restorative materials (Composite, Giomer, and Compomer) used. Groups were respresented as follows: Group I—ASB + Composite; Group II—ASB + Giomer; Group III—ASB + Compomer; Group IV—SBU + Giomer; Group V—SBU + Compomer; Group VI—SBU + Composite. After applying the bonding agent as per the manufacturer's instructions, following which the restorative material was placed. A Universal Testing Machine (Instron 3366, UK) was employed to estimate the shear bond strength of the individual restorative material and shear bond strengths were calculated. Results: Composite bonded with SBU (group VI) displayed the greatest shear strength (11.16 ± 4.22 MPa). Moreover, Giomers and flowable compomers displayed better bond strengths with ASB compared with their SBU–bonded counterparts. Conclusion: These results mark the importance of careful material selection in clinical practice and the bonding agent used to achieve optimal bond strength and enhance the clinical longevity and durability of dental restorations. Clinical significance: From a clinical perspective, to avoid a compressive or a shear failure, it would be preferrable to use a direct composite restorative material with SBU (Single bond universal adhesive, 7th generation) to achieve maximum bond strength.

495

ORIGINAL RESEARCH

D Bhanu UC Yadav, R Balagopal Varma, J Suresh Kumar, Parvathy Kumaran, Arun M Xavier, Malini Venugopal, Nishna Thankappan

Volumetric Analysis of Hand and Rotary Instrumentation, Root Canal Filling Techniques, and Obturation Materials in Primary Teeth Using Spiral CT

[Year:2024] [Month:March] [Volume:25] [Number:3] [Pages:10] [Pages No:250 - 259]

Keywords: Navitip Double Sideport, ProTaper, Spiral computed tomography

   DOI: 10.5005/jp-journals-10024-3644  |  Open Access |  How to cite  | 

Abstract

Aim and background: To compare the root canal volume in primary teeth using hand and rotary instruments and to evaluate root canal filling techniques and flow of root canal obturation materials in the postinstrumented root canal volume using spiral computed tomography (SCT). Materials and methods: Freshly extracted 16 primary molars were randomly divided into two groups and subjected to SCT analysis before and after instrumentation. For the manual technique (group I) with eight teeth were prepared using K files, and rotary (group II) eight teeth preparation was performed with ProTaper files. The filled volume in each canal was measured using SCT, and the percentage of obturated volume was calculated. The data were statistically analyzed using the Mann–Whitney U test. Results: There was a statistically significant difference in both groups’ volume of root canals enlarged. Even though both K files and the ProTaper system brought about enlarged canals after instrumentation, there was a statistically significant increase in volume after using K files in two canals. In three canals, there was a statistically significant increase in volume after using ProTaper. Irrespective of the obturation technique and materials used, there is no statistically significant difference in the volume after obturation. Conclusion: From the results of this study, the ProTaper file system shows suitable volumetric enlargement up to an optimum level, which is needed in primary root canal walls, and is better in canal shaping, as evidenced by good postobturation volume. Clinical significance: The traditional method of cleaning and shaping the root canals in permanent teeth using manual stainless-steel files can lead to undesirable curvatures in root canal morphology, making correctly filling the root canals difficult. It is also time-consuming and sometimes leads to iatrogenic errors. Rotary nickel–titanium (Ni–Ti) instrumentation techniques have been developed to overcome these problems.

314

ORIGINAL RESEARCH

Yan Mei Ng, S Nagarajan MP Sockalingam, Zaleha Shafiei, Ahmad Shuhud Irfani Zakaria, Alida Mahyuddin, Mariati A Rahman

Biological Activities of Virgin Coconut and Virgin Olive Oil Mixture against Oral Primary Colonizers: An In Vitro Study

[Year:2024] [Month:March] [Volume:25] [Number:3] [Pages:7] [Pages No:260 - 266]

Keywords: Antiadherence, Antibiofilm, Edible oils, Lactobacillus casei, Minimum bactericidal concentration, Minimum inhibitory concentration, Streptococcus mutans, Streptococcus sanguinis

   DOI: 10.5005/jp-journals-10024-3645  |  Open Access |  How to cite  | 

Abstract

Aim and background: This study aimed to explore the potential synergistic interaction of virgin coconut oil (VCO) and virgin olive oil (VOO) mixture against Streptococcus sanguinis, Streptococcus mutans, and Lactobacillus casei in a single and mixture species through the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), antiadherence, and antibiofilm activities. Materials and methods: The broth microdilution technique was used to individually determine the MIC of both oils and an oil mixture (in the ratio of 1:1) in a 96-well microtiter plate. As for the MBC, the subcultured method was used. The fractional inhibitory concentration index (ΣFIC) was determined to identify the interaction types between both oils. The oil mixture at its MIC was then tested on its antibiofilm and antiadherence effect. Results: The MIC of the oil mixture against the tested microbiota was 50–100%. The oil mixture was bactericidal at 100% concentration for all the mentioned microbes except S. mutans. The ΣFIC value was 2 to 4, indicating that the VCO and VOO acted additively against the microbiota. Meanwhile, the oil mixture at MIC (50% for S. sanguinis and L. casei; 100% for S. mutans and mixture species) exhibited antiadherence and antibiofilm activity toward the microbiota in mixture species. Conclusion: The oil mixture possesses antibacterial, antibiofilm, and antiadherence properties toward the tested microbiota, mainly at 50–100% concentration of oil mixture. There was no synergistic interaction found between VCO and VOO. Clinical significance: Children and individuals with special care may benefit from using the oil mixture, primarily to regulate the biofilm formation and colonization of the bacteria. Furthermore, the oil mixture is natural and nontoxic compared to chemical-based oral healthcare products.

445

ORIGINAL RESEARCH

Mohammad A Alrashidi, Manal F Badawi, Mohamed G Elbeltagy, Amany E Badr

The Effect of Glycyrrhizin on the Viability and Proliferation of Dental Pulp Stem Cells Compared to Intracanal Medicaments

[Year:2024] [Month:March] [Volume:25] [Number:3] [Pages:9] [Pages No:267 - 275]

Keywords: Calcium hydroxide, Dental pulp stem cells, Glycyrrhizin, Regenerative endodontics

   DOI: 10.5005/jp-journals-10024-3652  |  Open Access |  How to cite  | 

Abstract

Aim: To study the effect of glycyrrhizin (GA) on the viability and proliferation of dental pulp stem cells (DPSCs) compared with intracanal medicaments. Materials and methods: Third molars of an adult donor were used to obtain the DPSCs. Flow cytometry was utilized to conduct phenotypic analysis for DPSCs. The methyl-thiazol tetrazolium (MTT) test was used to detect the cell viability. Cell proliferation assay was conducted at distinct time intervals: 3, 5, and 7 days. Results: The flow cytometry analysis verified the positive expression of mesenchymal cell surface antigen molecules (CD73, CD90, and CD105) and the absence of hematological markers (CD14, CD34, and CD45) in the DPSCs. The cells that treated with concentrations more than 0.5 mg/mL of Ca(OH2) and triple antibiotic paste (TAP) gave significant decrease in viability in comparison to the untreated cells (p < 0.05). Also, the cells treated with concentrations 50 and 25 µM of GA showed no significant difference compared with the untreated cells (p > 0.05), while concentrations 12.5 and 6.25 µM expressed a significant increase in viability compared with the untreated cells (p < 0.05). At 7 days, cells treated with the three different concentrations of GA (12.5, 25, and 50 µM) demonstrated a significant increase in cell density compared with Ca(OH)2 and TAP-treated cells (p < 0.05). Conclusion: Based upon the potential of GA on DPSCs proliferation compared with Ca(OH)2 and TAP, It is conceivable to acknowledge that GA could be used as an intracanal medicaments for revascularization process of necrotic immature teeth. Clinical significance: This study emphasizes the significance of assessing alternative root canal medicaments and their impact on the proliferation and viability of DPSCs. The results regarding GA, specifically its impact on the viability and growth of DPSCs, provide essential understanding for its potential application as an intracanal medicine. This study adds to the continuous endeavors in identifying safer and more efficient intracanal therapies, which are essential for improving patient outcomes in endodontic operations.

353

ORIGINAL RESEARCH

Mohammad Jalaluddin, Pavithra K Ramanna, Monalisa Swain, Subhash Sonkesriya, Priyanka Rana, Deesha Kumari, Dina A A Derbala, Linda F Mirza, Shazia Mushtaq, Saiid E M Beshir

Evaluation of Fibrin Clot Interaction with Dental Implant after Different Surface Treatments: An In Vitro Study

[Year:2024] [Month:March] [Volume:25] [Number:3] [Pages:4] [Pages No:276 - 279]

Keywords: Dental implant, Fibrin clot, Surface treatment, Venous blood

   DOI: 10.5005/jp-journals-10024-3653  |  Open Access |  How to cite  | 

Abstract

Aim: The current study was carried out to assess the interaction between fibrin clots and dental implants following various surface treatments. Materials and methods: In this investigation, 45 dental implants with dimensions of 16 mm in length and 5 mm in diameter were utilized. They were divided up into three groups, each consisting of fifteen samples. Group I: Control; Group II: Ultraviolet (UV) light treated; and group III: Sandblasted and acid-etching (SLA) treated. Healthy volunteers’ venous blood samples were drawn into vacutainer tubes without the use of anticoagulants. The samples were centrifuged for 3 minutes at 2700 rpm in a table centrifuge. The entire implant was submerged in room-temperature liquid fibrinogen for 60 minutes. Then, scanning electronic microscopy (SEM) was used to examine each sample. The inter- and intragroup assessments were obtained using the Mann–Whitney U test and the Kruskal–Wallis test; p-values less than 0.05 were regarded as statistically significant. Results: The maximum adhesion of fibrin clot was found in SLA treated group (2.42 ± 0.10) followed by the UV light-treated group (2.18 ± 0.08) and control group (1.20 ± 0.02). There was a statistically significant difference found between the three surface-treated groups (p < 0.001). Conclusion: All surface-treatment methods exhibit adhesion between the implant surface and the fibrin clot. However, the highest adherence of fibrin clot was found in SLA treated group compared to the UV light-treated and control group. Clinical significance: The physical and chemical characteristics of an implant's surface have a significant impact on the way blood clots organize. At the interface between the implant and the bone, blood clot production can initiate and facilitate the healing process.

374

ORIGINAL RESEARCH

Supriya, Rajbir Singh, Amra Ahsan

Relevance of Emotion of Anxiety and Fear of Dentistry as Motivational Conflict in Oral Health Behaviors

[Year:2024] [Month:March] [Volume:25] [Number:3] [Pages:9] [Pages No:280 - 288]

Keywords: Dental anxiety, Dental fear, Health behavior, Health beliefs, Oral health, Oral health behaviors, Periodontitis

   DOI: 10.5005/jp-journals-10024-3643  |  Open Access |  How to cite  | 

Abstract

Aim: This study was undertaken with an aim to explore the influence of factors associated with anxiety and fear of dentistry on oral health behavior. Materials and methods: A total of 84 patients aged 20–40 years visiting the dental institute for the management of gum diseases (gingivitis and periodontitis) and tooth decay (dental caries) were enrolled. Fear of dentistry and oral health behaviors were recorded employing a dental fear survey (DFS) and oral health behaviors checklist. Each of the 20-item scale of DFS was rated on a 5-point Likert scale. The oral health behavior checklist was based on oral hygiene habits, patterns of utilization of dental services, food habits, and use of tobacco products. Each of the 13-item checklist comprised a closed-ended statement with a high score corresponding to more positive oral health behavior. Results: Domains of dental fear (avoidance of dentistry, physiological arousal, and fear of specific stimuli) and total dental fear did not predict oral hygiene habits and nutritional preferences (p > 0.05). Physiological arousal was a positive predictor of utilization of dental services (p = 0.009) and oral health behavior (p = 0.042). Oral health behaviors were found to be positively correlated with three factors of DFS. Conclusion: Anxiety and fear of dentistry are not found to influence personal preventive oral care with reference to oral hygiene habits. Avoidance of dentistry factor of DFS is positively correlated with oral health behavior. Dental fear and anxiety do not impact oral health behaviors adversely. Clinical significance: In this era of youth and beauty, the utilization of professional dental care services is not affected by fear of invasive nature of dental procedures. Establishing the groundwork for knowledge regarding the scope of fear appeals in anxiety for dentistry may help to augment positive oral health behaviors for effective primary prevention of oral diseases. Interactions among personality characteristics, attitudes, emotions, and health behavior need further exploration.

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CLINICAL TECHNIQUE

Sandeep K Madhu, Shiney Dominic, Joanna Baptist, Premalatha Shetty

Simple Method for Re-tightening IMF Wires without Breakage

[Year:2024] [Month:March] [Volume:25] [Number:3] [Pages:3] [Pages No:289 - 291]

Keywords: Intermaxillary fixation, Loop, Maxillofacial trauma, Occlusion

   DOI: 10.5005/jp-journals-10024-3650  |  Open Access |  How to cite  | 

Abstract

Aim: This clinical technique aims to retighten intermaxillary fixation (IMF) wires when loosened intra/postoperatively. Background: Intermaxillary fixation is one of the most important steps to obtain stable and functional occlusion in maxillofacial trauma. However, IMF wires tend to loosen over time. This loosened wire is generally removed and a new wire is used for IMF. Removal and refixation is time-consuming for surgeon and unconformable for the patient. Technique: We recommend a simple technique for re-tightening IMF wires without breakage, with the use of shepherd's crook explorer by making a small circular loop. Conclusion: This technique of re-tightening by looping further stretches and tightens the wire to regain stabilized occlusion with maximal intercuspation. Clinical significance: This technique eliminates the need for removal and refixation of IMF wires, thereby improving patient comfort, yet obtaining stable occlusion over a long period of time.

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