Aim: This study aims to examine the awareness, attitudes, and perceptions towards dental fluorosis in Hail, Saudi Arabia. Materials and methods: It was an epidemiological study with a descriptive cross-sectional design. Participants meeting the inclusion criteria were recruited from Hail, Saudi Arabia, through a convenient non-probability sampling strategy. The study planned to recruit at least 400 participants. However, (n = 419) was obtained during the 2 months of data collection. Data for the present study was collected using a validated questionnaire via Google form by disseminating it to the targeted population using non-probability snowball sampling through various social media platforms. The questionnaire comprised three parts including demographics (6 questions), a knowledge-related section comprised of (6 questions), and attitude relation portion (14 questions). Results: The majority of participants (n = 369, 88.1%) were willing to undergo treatment for dental improvement. Additionally, most respondents (n = 389, 92.8%) advocated for government participation in preventive programs and emphasized the importance of raising public awareness. Furthermore, a significant proportion (n = 401, 95.7%) expressed interest in launching mass public health campaigns to prevent dental fluorosis. Females showed significantly higher awareness levels. Conclusion: The study highlights significant sociodemographic influences on awareness and attitudes towards dental fluorosis. While age, nationality, and income showed no impact, gender and education were significant factors. Despite gaps in knowledge, there was strong support for preventive measures. Esthetic concerns were evident, with high rates of dental consultations and recognition of social stigma. Targeted interventions are needed to address knowledge gaps and promote preventive measures, considering sociodemographic differences and broader social implications. Clinical significance: The findings could be the cornerstone for promoting preventive measures and improving consultation behaviors, ultimately enhancing the overall management and prevention of dental fluorosis in the community.

Aim: The current study aims to assess the color change of polymer-infiltrated ceramic Vita Enamic (VE) and extra translucent multilayer zirconia (XTML) after being immersed in different types of beverages, which are coffee, tea, and cola in comparison to distilled water as control. Materials and methods: A total of 80 rectangular-shaped specimens were prepared with fixed dimensions (14 × 12 × 0.5 mm) and then were divided into two groups (n = 40) according to ceramic material (VE, XTML). Specimens were sliced as each slice measures about 0.5 mm thick. Each group specimens were divided into four subgroups (n = 10) based on the immersion solutions in which specimens were stored (water, coffee, tea, and cola) for 28 days. The color parameters (L-a-b) of the specimens were recorded before immersion and at the end of the 7th (T1), 14th (T2), 21st (T3) and 28th (T4) days after immersion. Color measurements were statistically analyzed with a significance threshold of p < 0.05. Results: There was a significant difference in color change between VE and XTML in all periods of tea and coffee immersion subgroups and in T3 and T4 in cola immersion subgroups (p < 0.001). Vita Enamic showed the highest differences in ΔE through all storage periods after 28 days of tea immersion (ΔE of VE= 8.06 ± 1.04). Extra translucent multilayer zirconia showed the highest differences in ΔE through all storage periods after 28 days of tea immersion (ΔE of XTML = 3.0 ± 0.33). Conclusion: Commonly consumed staining beverages influenced the color stability of the polymer-infiltrated ceramics more than extra translucent zirconia ceramics. Clinical significance: This study may provide guidance for clinicians to select the appropriate ceramic restorative material with high color stability and low tendency for color change by common staining beverages to achieve long-lasting esthetic results for the patients.

Aim: This study aimed to evaluate the effect of adding different concentrations of silver nanoparticles (AgNPs) on the flexural strength and microhardness of various denture base materials. Materials and methods: For this study, a total of 60 specimens were used and divided into equal groups. The first group consisted of heat-cured acrylic resin (Vertex-Germany), while the second group consisted of thermoplastic resin (Breflex 2nd edition, Germany). The samples were created using a split brass mold with dimensions of 65 × 10 × 2.5 mm, in accordance with the specifications of the American Dental Association (specifically No. 12 for flexural and microhardness). Following this, the samples were divided into three groups (A, B, and C) based on different concentrations of AgNPs (0, 2, and 5%). The flexural and microhardness of the samples were assessed using a universal testing machine and the Vickers hardness test, respectively. The data were gathered, organized, and analyzed using statistical methods. Results: The flexural strength findings showed a significant difference between the two groups. Also, there was a considerable decrease in the average value of the acrylic group as the concentrations of AgNPs rose, while the flexural strength of the thermoplastic group notably improved. Regarding microhardness, the results showed a significant difference between the two groups. It showed that the mean value of both groups increased with increasing concentrations of AgNPs. Conclusion: Within the limitations of laboratory testing conditions of this study, it was discovered that AgNPs negatively impact the flexural strength of acrylic resins. Furthermore, an increase in the concentration of AgNPs was found to be directly related to the flexural strength of thermoplastic resin and the microhardness of both groups. Clinical significance: The concentration of AgNPs has a significant impact on certain mechanical properties of denture base materials, but it is important to consider their potential toxicity.

Aim: This study compared the quality and quantity of newly formed bone in rabbits’ critical-sized calvarial defects filled with enamel matrix derivative (EMD) combined with freeze-dried bone allograft (FDBA) vs FDBA alone. Materials and methods: A total of 24 adult male white New Zealand rabbits were included. In each rabbit, three bone defects with a diameter of 8 mm were created on the calvarium bone; the first defect was left untreated, while the second was filled with FDBA, and the third was filled with EMD + FDBA. Twelve rabbits were randomly euthanized after a month, and the remaining 2 month postsurgery. Bone sections were histologically evaluated by hematoxylin and eosin and vascular endothelial growth factor (VEGF), alkaline phosphatase (ALP), osteoprotegerin (OPG), and receptor activator of NF-kappaB (RANK) immune-histochemical staining. Results: An improvement in the newly formed bone percentage was found in the defects filled with EMD + FDBA in comparison with FDBA and control defects at 1 month and 2 months postsurgery. Additionally, the expression of VEGF, ALP, OPG, and RANK showed highly significant differences in the defects filled with EMD + FDBA compared to the FDBA and control ones at 1 month postsurgery (p = 0.001). Meanwhile, VEGF and ALP expression showed a significant decrease in defects filled with EMD + FDBA compared to the FDBA and control ones (p = 0.001), while OPG and RANK expression showed non-significant differences between treated groups at 2 months postsurgery. Conclusion: Enamel matrix derivative combined with FDBA has a synergistic effect on bone formation and graft substitution. This combination accelerates the expression of VEGF, ALP, OPG, and RANK. Clinical significance: The combination of EMD and FDBA accelerates and ameliorates the quality of newly formed bone, aiding in maxillofacial reconstruction.

Aim: The present study clinically analyzes implant survival of immediate implant placement cases using the drilling through roots (DTR) technique for anatomically-guided implant site preparation, as an aid to placing immediate dental implants in multi-radicular teeth. Materials and methods: This clinical analysis utilized patients’ electronic dental records who underwent immediate implant surgery using the DTR technique. All immediately placed implants were followed up regularly every year, after restoration. Implant survival was assessed with the Albrektsson et al. criteria. Inferential statistics was performed using SPSS v 21(IBM Corp., Armonk, NY) software. The Kaplan–Meier survival analysis was done to assess the implant survival probability. Results: A total of 250 records of dental implants placed in 227 subjects using the DTR technique were considered. Results showed that the mean survival duration of implants was found 63.29 months and the median survival duration to be 55 months. A 100% success rate was seen in implant fixed bridge cases, and about 97.6% success was seen in single crown cases. No significant difference was seen in the survival rates during the follow-up period when compared according to the quadrants/site of implant placement. Conclusion: The findings concluded that tooth-guided rapid implant placement is a unique strategy for convenient and safe insertion, providing accurate three-dimensional positioning. Clinical significance: The DTR method is a novel approach that facilitates accurate positioning and angulation of the implant bed preparation by stabilizing and guiding the osteotomy drills using the retained root. As a result, it enables optimal implant positioning at multirooted extraction sites.

Aim: This study aims to compare the effectiveness of “omega-3 fatty acids” as an auxiliary to “scaling and root planing (SRP)” with traditional “scaling and root planing” in periodontal treatment in humans. Materials and methods: This study is a randomized control trial and was carried out over a period of 3 months (registered on 02/07/2023). Thirty patients were singled out according to the inclusion criteria, each having periodontitis (Stage II Grade B), and were arbitrarily distributed into two groups (control and test). The test group was treated with “scaling and root planing” along with the adjunctive application of “omega-3 fatty acids” while the control group was treated with “scaling and root planing” alone. Monthly follow-up was carried out over 90 days. Clinical parameters such as pocket probing depth (PPD), gingival index (GI), bleeding index (BI), and plaque index (PI) were measured respectively at baseline and 3 months. The data was recorded and statistically analyzed. Results: The soft tissue architecture remained stable. The mean full mouth plaque index (FMPI) score was statistically significant (p < 0.001) when the control group was compared to the test group with a mean difference of 0.12 ± 0.02. The mean full mouth papillary bleeding index (FMPBI) score decreased at 3 months and was statistically significant compared to baseline with a mean difference of 0.24 ± 0.04 (p < 0.001). When the test group was compared with the control group, the FMGI was not significant (p = 0.02), with a mean difference of 0.16 ± 0.19. The PPD was not significant (p =1) when comparing both the groups, with a mean difference of 0 ± 0.66. Although the clinical parameters were statistically significant at 3 months when compared to baseline in both the groups, the FMGI and PPD were not significant. Conclusion: The combined action of using omega-3 fatty acid as an auxiliary to conventional scaling and root planing improved the periodontal parameters including both the soft and hard tissue outcomes. Clinical significance: The present study indicated that supplementary usage of omega-3 fatty acids is more beneficial for treating chronic and mild periodontitis than scaling and root planing alone. Omega-3 fatty acids can be used as energy for our cells, reduce the risk of blood clotting, maintain bone health, regulate metabolism, and reduce inflammation. Host modulatory therapy (HMT) with omega-3 fatty acids aims at reducing inflammation. With HMT as an adjunct, a better result of periodontal therapy was expected. It enhanced the positive effects on periodontal parameters and both the soft and hard tissue outcomes.

Aim: The study aims to compare the effectiveness of scaling root planing alone and scaling root planing with oxygen-releasing gel in the treatment of chronic periodontitis. Materials and methods: A split-mouth randomized controlled trial was designed on 25 systemically healthy participants with 50 sites having chronic periodontitis. Two sites were selected for each patient and were randomly allocated into two groups. A total of 50 sites were selected and divided into two groups with 25 test sites in group I (Test group) and 25 control sites in group II (Control group). Group I received scaling and root planing (SRP) followed by placement of BlueM oral gel, while group II received SRP alone. Probing pocket depth (PPD), clinical attachment level (CAL), gingival index (GI), and bleeding on probing (BOP) were recorded at baseline and after 6 weeks and were compared accordingly. Results: The results revealed a reduction in PPD, CAL, GI, and BOP in both groups. However, the test group showed a statistically significant reduction when compared with the control group in the above-mentioned clinical parameters. Conclusion: The results suggested that the use of oxygen-releasing gel with SRP provided additional benefits in the management of chronic periodontitis by promoting greater reductions in the clinical parameters. Clinical significance: BlueM oral gel, i.e. the oxygen-releasing gel as a local drug delivery, may be an excellent adjunct to SRP in treating chronic periodontitis.

Aim: To evaluate the morphology of the mastoid process and its role in sex determination with the help of cone-beam computed tomography (CBCT). Materials and methods: A retrospective study was conducted on 200 adults who were subjected to full FOV CBCT scans (100 males and 100 females). Eight parameters of the mastoid process were assessed for sex determination. The study measured eight parameters of the mastoid process, including mastoid length (ML), mastoid width, mastoid height (MH), intermastoidale distance (IMD), intermastoidale lateral surface distance (IMLSD), mastoid medial convergence angle, mastoid size (MS), and mastoid surface area (MSA). Data were recorded and statistically analyzed. Results: Statistics revealed a significant value for MH (p < 0.001), IMD (p < 0.001), IMLSD (p < 0.001), MSA (p < 0.001), MS (p < 0.001), and ML (p = 0.032). A highly significant value was noted for MH (p < 0.001), IMD (p < 0.001), IMLSD (p < 0.001), MSA (p < 0.001), MS (p < 0.001), followed by ML, all of which were found to be greater in males. Out of the eight parameters, IMD was found to be the best sex determinant among all the eight parameters, with an accuracy of 70%. The study showed a significant difference between the mastoid process morphometric measurements for males and females. Conclusion: It can be concluded from the present study that IMD can be used as a good index for sex determination. The combined parameters that were found to be the most accurate were right MH, left ML, and IMLSD, with an accuracy of 75%. Clinical significance: The three-dimensional imaging techniques can contribute significantly towards disaster victim identification and sex determination in the fields of forensic odontology and anthropology. For the recognition of victims, sex determination becomes one of the most difficult parameters to assess. In such events, the mastoid process can become an important anatomical landmark for the estimation of sex. This is due to the condensed nature of the petrous bone and its protected position in the skull.

Aim: This study aimed to evaluate the reporting quality of systematic review (SR) abstracts in leading orthodontic journals using the PRISMA abstract criteria. Additionally, the study examined characteristics associated with improved abstract reporting quality. Materials and methods: A retrospective observational study design was employed. Systematic reviews published between January 2018 and December 2022 in four prominent orthodontic journals were identified through electronic and manual searches. Inclusion criteria focused on articles with “SR” or “meta-analysis” keywords in the title or abstract. Narrative and historical reviews, scoping reviews, and case reports with extensive literature reviews were not considered as part of the exclusion criteria. The screening was carried out in duplicate and independently by the two authors. Results: The European Journal of Orthodontics had the highest number of included articles, while the Journal of Orthodontics had the lowest. The majority of SRs had authors affiliated with academic institutions. Compliance scores varied across journals and regions, with Asia scoring the highest. Certain checklist items, such as identifying the report as an SR, stating objectives, describing included studies, providing interpretation, and registration, were adequately reported in over 93% of the reviews. However, the reporting of risk of bias and synthesis of results showed room for improvement. Conclusion: The study revealed a significant improvement in the overall Preferred Reporting Items for SRs and Meta-Analyses for Abstracts (PRISMA-A) score of included SRs, primarily due to enhanced reporting of specific checklist items. However, there remains considerable scope for further improvement in abstract reporting, highlighting the importance of striving to meet higher standards in SR abstracts. Clinical significance: The study showed a notable increase in the PRISMA-A score. However, there is still a need for continued efforts to meet higher reporting standards in SR abstracts.

Background: Healthcare embraces simulation's learning boost, from medicine to dentistry. But can it unlock the secrets of intricate fixed prosthodontics? This study takes a pioneering step to find out. Aim: To evaluate low-fidelity medical simulation (LFMSim) in fixed prosthodontics by assessing its effectiveness in training corono-radicular preparations and by exploring participants’ perceptions of LFMSim as an innovative teaching tool for developing pre-practice skills. Materials and methods: A prospective, descriptive study was executed to explore these facets among 6th-year students. The Debriefing Assessment for Simulation in Healthcare (DASH©) evaluation grid, student version, was employed for assessment. The study encompassed one week, from 03/03/2023 to 10/03/2023. Results: The study involved 28 learners with an average age of 23 and a sex ratio of 0.33, half of whom had prior exposure to simulation sessions. Seventy-five percent of participants watched the instructional video a day before the simulation, with nine expressing presession stress. Overall, 19% of students found the session as expected, while 43% considered it better than anticipated. Satisfaction levels varied, with 50% indicating they were “rather satisfied,” and 46.4% expressing absolute satisfaction. The Debriefing Assessment using DASH yielded an average score of 4.85. Conclusion: The study's findings shed light on the effectiveness of LFMSim in training corono-radicular preparations and post-space impressions for fixed prosthodontics among 6th-year students. The varied satisfaction levels and the emphasis on debriefing discussions suggest avenues for improving simulation sessions in the future. Clinical significance: These insights are pertinent for educators and institutions aiming to enhance dental education through innovative teaching tools like simulation. By refining simulation sessions based on debriefing feedback, educators can better prepare students for real-world clinical scenarios, ultimately improving patient care outcomes. This highlights the practical importance of incorporating simulation-based learning into dental curricula.

Aim: To assess the gloss and gloss retention of two esthetic restorative materials after brushing with a charcoal-infused toothbrush and activated charcoal toothpaste compared to their standard counterparts in all possible combinations. Materials and methods: In this study,104 disc-shaped specimens were prepared, 52 from each of the two materials: Beautifil II LS composite resin (Giomer) and Fuji II LC resin-modified glass ionomer (RMGI). Specimens of each material were divided into 4 groups (n = 13) according to the tested brushing procedure: group A—standard toothbrush + standard non-whitening toothpaste (S-S), group B—standard toothbrush + activated charcoal toothpaste (S-CH), group C—charcoal-infused toothbrush + standard non-whitening toothpaste (CH-S), and group D—charcoal-infused toothbrush + activated-charcoal toothpaste (CH-CH). Gloss was recorded using a gloss meter initially and after each brushing protocol. The data were statistically analyzed with two-way analysis of variance (ANOVA) and Tukey HSD post hoc tests using SPSS^® v.27 software at 5% significance level. Results: Beautifil II LS showed the highest gloss values after brushing in group D (44 ± 3.9), which was not significantly different from its initial value (46.1 ± 1.8), followed by groups A (32.2 ± 4), B (23.2 ± 3.6), and C (22.7 ± 3.9), while Fuji II LC showed its highest gloss values after brushing in groups D (16.6 ± 3.7) and a (15.4 ± 8.7), followed by groups C (10.9 ± 6) and B (4.4 ± 2.5), all were significantly lower than their respective initial gloss values. Conclusion: The gloss of Giomer and RMGI reduced significantly following the brushing with the tested brushing procedures except for the Giomer group brushed with a combination of charcoal-infused toothbrush and activated-charcoal toothpaste. Clinical significance: The brushing using a combination of charcoal-infused toothbrush and activated-charcoal toothpaste is recommended for the gloss retention of Beautifil II LS restorations. However, the gloss of the Fuji II LC cannot be retained after any of the brushing procedures.

Aim: Endodontically treated teeth often require 360° ferrule to retain post and core restoration. The literature support on scientific evidence of partial ferrule is limited, hence this study was conducted to evaluate the fracture resistance of two post and core systems, i.e., Edelweiss and glass fiber post and core system with varying ferrule locations. Materials and methods: About 54 extracted mandibular 1st premolars were divided into Edelweiss post and core (Group A) and glass fiber post and core (Group B). They were further subdivided into 3 groups each (Partial functional, partial non-functional, and circumferential). A standardized endodontic procedure was carried out on all the specimens. For group A, an Edelweiss drill of 1.4 mm was used to prepare the post space. Canals were then etched, a bonding agent was applied and posts were luted with paracore resin. For group B, post space was prepared with a straight fissure bur, followed by the same procedure as that of group A. Core build-up was done with composite resin. Cast crowns were fabricated and cemented with GIC. Fracture resistance was evaluated with UTM with a compressive load capacity of 1000 N until fracture occurred. Results: The results of this study stated that in the Edelweiss group partial functional ferrule showed higher fracture resistance and in the glass fiber group circumferential ferrule showed higher fracture resistance. The study also showed that MOF is favorable in all the groups. Conclusion: The type of ferrule was one of the factors that affected the fracture resistance of Edelweiss and Glass fiber post and core group of specimens. The study also concluded that there was a predominant favorable mode of fracture across all groups, in all different ferrule locations. Clinical significance: This study opens multiple avenues and methods in tooth restoration and shows that even a partial functional ferrule is sufficient for restoration, provided the right system is used.

Aim and objectives: To characterize and analyze the structural presentation of a new denture base copolymer with a spiro-acetal cross-linker at 10 and 20 wt.% concentrations by nuclear magnetic resonance (NMR) and field emission scanning electron microscopy-energy-dispersive X-ray (FESEM-EDX) spectroscopies. Also, to evaluate the glass transition temperature (T_G) of the new copolymer. Materials and methods: The investigational groups G10 and G20 were heat-cured with the new spiro-acetal cross-linker at the above-mentioned concentrations, respectively. The control group G0 was heat-cured without the new cross-linker. Nuclear magnetic resonance and EDX spectroscopies determined the copolymerization along with elemental composition. The surface characteristics were discerned by FESEM. Differential scanning calorimetry was employed to evaluate the T_G of the resultant copolymer. Appropriate statistical operations were performed to compare the mean T_G of the groups. Results: The new copolymer's structure with the spiro-acetal cross-linker was configured with protons, carbons, aluminum, zirconium, yttrium, and silicon atoms. The T_G of the resultant copolymer was high when compared with the G0. The 20 wt.% spiro-acetal cross-linker in the copolymer exhibited the highest T_G. Conclusion: The spiro-acetal cross-linking comonomer incorporated in the heat-cure denture polymer produced a new denture base copolymer with elevated T_G. The resultant configuration of the new copolymer was characterized, structurally presented, and confirmed. Clinical significance: The new copolymer might exhibit augmented strength due to the copolymerized spiro-acetal cross-linker. Moreover, the smooth and regular surface of the copolymer would have minimum or negligible microbial adhesion due to the hydrophobicity of the spiro-acetal comonomer incorporated in the denture base composition.

Aim: The current study aimed to assess the efficiency of two desensitizing dentifrices on the management of dental hypersensitivity. Materials and methods: For the purpose of this investigation, 60 extracted human sound premolar teeth that were removed for orthodontic purposes were collected. On the buccal cervical areas, an inverted-cone bur was used to create cavities that were 2 mm deep and 3 mm wide. The blocks were submerged in 17% ethylenediaminetetraacetic acid (EDTA) for 40 minutes in order to ensure the complete dentin tubule opening. Following preparation, all samples were split into three groups, each containing 20 samples, Group A: Control, Group B: Dentifrice containing calcium sodium phosphosilicate, Group C: Dentifrice containing casein phosphopeptide–amorphous calcium phosphate (CPP–ACP). Scanning electron microscopy (SEM) was used to assess the occlusion of dentinal tubules. One-way analysis of variance (ANOVA) was used to assess the desensitization efficacy of dentifrices. At a p-value less than 0.05, statistical significance was determined. Result: Before application of different dentifrices, the maximum dentinal tubules opened in dentifrice containing CPP–ACP group (4.24 ± 0.10) followed by control group (4.18 ± 0.01) and dentifrice containing calcium sodium phosphosilicate (4.12 ± 0.06). And there was no significant difference between the different dentifrice groups (p > 0.001). After application of different dentifrices, the highest occlusion of dentinal tubules found in dentifrice containing CPP–ACP group (2.50 ± 0.05) followed by dentifrice containing calcium sodium phosphosilicate (2.84 ± 0.10) and control group (4.02 ± 0.07) and there was a highly significant difference between the different dentifrice groups (p < 0.001). Conclusion: On conclusion, dentifrice containing CPP–ACP exhibited the highest level of dentinal tubule occlusion in comparison to the control group and dentifrice containing calcium sodium phosphosilicate. Clinical significance: Dentinal hypersensitivity (DH) is a condition that is frequently experienced. With variable outcomes, a number of products are utilized in the management of DH. Need is felt in dentistry for a material that chemically reacts, physically occludes and adheres intimately to dentinal tubules to reduce the possibility of its recurrence.

Aim: The aim of the present study was to estimate the salivary copper levels in oral submucous fibrosis (OSMF) condition. Materials and methods: A total of 60 individuals of which an equal number of 30 each in normal healthy control group as well as in clinically and histopathologically (the biopsy was done once after the clinical confirmation of OSMF in the patient) confirmed patients of OSMF were included in the study group. Total of 51 males and 9 females were considered for the present study and the age distribution of these groups ranged from a minimum of 21 years to a maximum of 74 years. Unstimulated whole saliva was collected from the patient followed by the conventional biopsy practice. The collected saliva was then subjected for the analysis of copper levels. Trace element copper was estimated by using Digital Semiautomatic Analyzer with the help of copper kit. The clinical mouth opening of OSMF was estimated. Analysis of variance (ANOVA) and Tukey HSD post hoc test was used analyze the data wherein the participants were grouped into age ranges of 20–30, 31–40, 41–50, and >60 years. Results: The mean salivary copper level among OSMF and control groups with respect to age in 20–30 years was 55.98 ± 15.50 and 30.87 ± 7.70, in 31–40 years was 63.96 ± 21.13 and 32.95 ± 4.56, in 41–50 years was 50.11 ± 6.83 and 30.46 ± 3.28, and >60 years was 45.65 and 13.67 µg/dL, respectively. The mean salivary copper levels among OSMF and Control groups with respect to males were 55.60 ± 15.27 and 31.18 ± 6.97 and among females were 67.0 ± 24.25 and 30.06 ± 5.77 µg/dL, respectively. The mean salivary copper levels with histopathological grades in very early stage was 47.18 ± 5.73, in early stage was 49.22 ± 7.65, in moderately advanced was 73.53 ± 10.62 and in OSMF with mild dysplasia was 79.98 ± 16.27 µg/dL, respectively. The mean salivary copper levels in individuals with clinical mouth opening more than 35 mm was 45.65 ± 6.57, in 25–35 mm was 48.94 ± 21.60, in 15–25 mm was 70.54 ± 3.52 and in less than 15 mm was 81.50 ± 16.66, respectively. Conclusion: The present study concluded that salivary trace element levels could be used as potential diagnostic and prognostic markers in patients with OSMF. Clinical significance: Trace elements are involved in many different physiological and metabolic processes in humans, either directly or indirectly. Copper is involved in vital biochemical activities like different redox and free radical formation and in maintaining cellular proton homeostasis. It is also associated with the processing of oxygen and a component of arecanut in all forms, which is implicated in the etiology of OSMF.