The Journal of Contemporary Dental Practice

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2002 | August | Volume 3 | Issue 3

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RESEARCH ARTICLE

James D. Kettering, Joni A. Stephens, Carlos A. Muñoz-Viveros, W. Patrick Naylor

Reducing Bacterial Counts in Dental Unit Waterlines: Tap Water vs. Distilled Water

[Year:2002] [Month:August] [Volume:3] [Number:3] [Pages:11] [Pages No:1 - 11]

   DOI: 10.5005/jcdp-3-3-1  |  Open Access |  How to cite  | 

Abstract

Background

The maximum recommended level of microbial contamination of water from dental unit waterlines (DUWL) is 200 colony-forming units per milliliter (CFU/mL). This article addresses the importance of water selection in achieving that standard.

Methods

Microbial contamination in water samples from 75 new dental units, with a closedcircuit water system, were compared using combinations of tap water and sterile distilled water with and without two chemical disinfectants (bleach and 0.12% chlorhexidine gluconate, Bio2000) over a six-week period. Baseline tap water samples were collected and tested initially.

Results

The microbial plate counts of seven tap water specimens (controls) ranged from 4 to 95 CFU/mL. These results were well below both the 500 CFU/mL standard for public drinking water and the 200 CFU/mL goal for dental treatment water. However, when passed through dental units, no significant bacterial reduction was achieved for samples of tap water (Group 1), tap water treated with bleach (Group 2), or tap water treated with Bio2000 (Group 4). Only water samples from dental units using Bio2000 alone (Group 3) or a combination of sterile, distilled water with Bio2000 (Group 5) met or exceeded the 200 CFU/mL standard.

Conclusions

Using tap water alone or tap water with bleach did not improve water quality. However, the American Dental Association (ADA) standard for reduced microbial contamination of dental unit waterlines was met using Bio2000 and distilled water treated with Bio2000.

Clinical Significance

The ADA standard of 200 CFU/mL was achieved using a closed water system and distilled water treated with Bio2000. Using 100% Bio2000 is also effective, but more costly.

Citation

Kettering JD, Stephens JD, Muñoz-Viveros CA, et. al. Reducing Bacterial Counts in Dental Unit Waterlines: Tap Water versus Distilled Water. J Contemp Dent Pract 2002 August;(3)3: 001-009.

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RESEARCH ARTICLE

Jane L. Forrest, Syrene A. Miller

Evidence-Based Decision Making in Action: Part 1 - Finding the Best Clinical Evidence

[Year:2002] [Month:August] [Volume:3] [Number:3] [Pages:17] [Pages No:10 - 26]

   DOI: 10.5005/jcdp-3-3-10  |  Open Access |  How to cite  | 

Abstract

Citation

Forrest JL, Miller SA. Evidence-Based Decision Making in Action: Part 1 - Finding the Best Clinical Evidence. J Contemp Dent Pract 2002 August;(3)3: 010-026.

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RESEARCH ARTICLE

Khalid Almas

The Effect of Salvadora Persica Extract (Miswak) and Chlorahexidine Gluconate on Human Dentin: A SEM Study

[Year:2002] [Month:August] [Volume:3] [Number:3] [Pages:9] [Pages No:27 - 35]

   DOI: 10.5005/jcdp-3-3-27  |  Open Access |  How to cite  | 

Abstract

Citation

Almas K. The Effect of Salvadora Persica Extract (Miswak) and Chlorhexidine Gluconate on Human Dentin: A SEM Study. J Contemp Dent Pract 2002 August;(3)3: 027-035.

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RESEARCH ARTICLE

Mary Ann Haisch, Nora L. Cromley

Mentoring: A Professional Responsibility

[Year:2002] [Month:August] [Volume:3] [Number:3] [Pages:10] [Pages No:36 - 45]

   DOI: 10.5005/jcdp-3-3-36  |  Open Access |  How to cite  | 

Abstract

Citation

Cromley NL, Haisch MA. Mentoring: A Professional Responsibility. J Contemp Dent Pract 2002 August;(3)3: 036-045.

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